<p>The Effect of Exogenous Superoxide Dismutase (SOD) on Caspase-3 Activation and Apoptosis Induction in Pc-3 Prostate Cancer Cells</p>

Abstract

Background This study aimed to assess the effects of exogenous SOD administration on prostate cancer cell line (PC-3) apoptosis via the intrinsic pathway by examining the expression of manganese superoxide dismutase (MnSOD), caspase-3, and apoptosis index of the PC-3 cell line. Methods We used the prostate cancer cells from secondary prostate cancer cell lines (PC-3) derived from castration refractory prostate cancer (CRPC), cell differentiation grade IV, and had metastasized to the bone from the American Type Culture Collection (ATCC, Rockville, MD, USA). Superoxide dismutase (SOD) is derived from extracts of melon seeds and wheat gliadin biopolymer, and divided into 62.5 mg/mL, 83 mg/mL, 125 mg/mL, and 250 mg/mL doses. Expression of MnSOD was measured by immunohistochemistry (IHC). Expression of caspase-3 was measured using Western Blot method. Apoptotic index is calculated based on the reaction introduction 3OH end of fragmentation of DNA by the enzyme terminal transferase in preparations with TUNEL staining reagents. A one-way ANOVA test and Pearson correlation test were used to determine the relationship between SOD with expression of caspase-3 and apoptotic index. Results SOD extract significantly increased the expression of caspase-3 ( P =0.016) and the apoptotic index ( P =0.000) ( P <0.05). There was a correlation between the increased doses of SOD extract and the apoptosis index ( P =0.015; r=0.679) and between the increased caspase-3 expression and the apoptosis index ( P =0.015; r=0.682). Conclusion Administration of superoxide dismutase (SOD) increased apoptosis in a prostate cancer cell line (PC-3) through the increased expression of caspase-3. Superoxide dismutase (SOD) can be considered as a therapy for late-stage prostate cancer that had been progressed to hormone resistant and metastasized and promote apoptosis in those prostate cancer cells.

Impact of chitosan administration on titanium dioxide nanoparticles induced testicular dysfunction

“…Moreover, GST is an enzyme that is required for the protection against DNA damage induced by apoptosis, where exhaustion of GST activity can be found due to the incidence of apoptosis. Superoxide dismutase is an enzyme that is associated with a defense mechanism against free radicals, found to decrease in the case of prostate carcinoma 32 , and found to be effective when supplemented to decrease apoptosis. The downregulation of catalase as well as the decrease in its activity might inhibit the process of apoptosis 33 ; due to overproduction of hydrogen peroxide.…”

The Effect of Encapsulated Apigenin Nanoparticles on HePG-2 Cells through Regulation of P53

“…The superoxide dismutase (SOD) enzyme produced within healthy cells can convert superoxide radicals to H 2 O 2 . Excess induction of SOD in cancer cells can induce apoptosis [93]. In contrast, increased catalase (CAT) can protect the tumor cell from excess ROS.…”

Beneficial Effect of Methanolic Extract of Frankincense (Boswellia Sacra) on Testis Mediated through Suppression of Oxidative Stress and Apoptosis

“…However, earlier reports suggest that Bcl-2 does not change the activity of SOD [ 39 ] while an increase in catalase activity associated with a reduced expression of caspase-3 has been reported in many tissues [ 40 , 41 ]. On the contrary, administration of exogenous SOD is reported to induce expression of caspase-3 in prostate cancer cells lines [ 42 ].…”