&lt;p&gt;Systemic Cytokine Profiles of CD4+ T Lymphocytes Correlate with Clinical Features and Functional Status in Stable COPD&lt;/p&gt;

Uzeloto, Juliana Souza; Toledo-Arruda, Alessandra Choqueta de; Silva, Bruna Spolador de Alencar; Golim, Márjorie de Assis; Braz, Aline Márcia Marques; Lima, Fernando Parra dos Anjos; Grigoletto, Isis; Ramos, Ercy Mara Cípulo

doi:10.2147/copd.s268955

Cited by 7 publications

(7 citation statements)

References 45 publications

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“…Moreover, we found a further increase in IFN-γ, GM-CSF, IL-8, and IL-6 levels in BCO patients compared those in COPD patients, suggesting that airflow obstruction could play a role in worsening the inflammatory process. In accordance with our data, Uzeloto et al evaluated the expression of intracellular cytokines in CD4+ T lymphocytes, finding that individuals with greater bronchial obstruction presented a higher proportion of IL-8 [ 34 ]. On the contrary, IL-10 levels were lower in our cohort of patients than in healthy subjects.…”

Section: Discussionsupporting

confidence: 90%

Adiponectin Increase in Patients Affected by Chronic Obstructive Pulmonary Disease with Overlap of Bronchiectasis

Nigro

Mosella

Daniele

et al. 2023

Life

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Chronic obstructive pulmonary disease (COPD) is characterized by respiratory symptoms and non-reversible airflow limitation with recurrent episodes of acute exacerbations. The concurrent presence of bronchiectasis in patients with COPD is associated with reduced respiratory function as well as increased exacerbation risk. Adiponectin is a promising biomarker in COPD, as greater high molecular weight (HMW) oligomer levels have been observed among COPD patients. Here, we investigate adiponectin levels in two groups of COPD patients characterized by the presence or absence of bronchiectasis (BCO), comparing both groups to healthy controls. We evaluated serum adiponectin levels in COPD patients, those with BCO, and healthy subjects and characterized the pattern of circulating adiponectin oligomers. We found that forced volume capacity % (FVC%) and forced expiratory volume % (FEV1%) were lower for BCO patients than for COPD patients. COPD patients had higher levels of adiponectin and its HMW oligomers than healthy controls. Interestingly, BCO patients had higher levels of adiponectin than COPD patients. We showed that expression levels of IL-2, -4, and -8, IFN-γ, and GM-CSF were significantly higher in BCO patients than in healthy controls. Conversely, IL-10 expression levels were lower in BCO patients. Our data suggest that the increased levels of adiponectin detected in the cohort of BCO patients compared to those in COPD patients without bronchiectasis might be determined by their worse airway inflammatory state. This hypothesis suggests that adiponectin could be considered as a biomarker to recognize advanced COPD patients with bronchiectasis.

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Section: Discussionsupporting

confidence: 90%

Adiponectin Increase in Patients Affected by Chronic Obstructive Pulmonary Disease with Overlap of Bronchiectasis

Nigro

Mosella

Daniele

et al. 2023

Life

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“…The procedures performed for labeling CD3+ (anti-CD3 FITC) and CD4+ (anti-CD4 PerCP-CY5.5) as well as for labeling the specific monoclonal antibodies for the cytokines of interest [anti-interleukin (IL)-8 PE, IL-13 APC, IL-17 PE, IL-6 APC, IL-2 APC, IL-10 PE, tumor necrosis factor (TNF) APC] were previously described. 33 All reagents used in the analyses were from Becton Dickinson, San Diego, CA.…”

Section: Methodsmentioning

confidence: 99%

“…The strategy for identifying lymphocytes as well as for evaluating the expression of intracytoplasmic cytokines has been previously described. 33 The analyses were performed in CellQuest PRO ® and FlowJo ® software, with the results expressed in percentage values.…”

Section: Methodsmentioning

confidence: 99%

Effect of physical training on cytokine expression in CD4+ T lymphocytes in subjects with stable COPD

Uzeloto

Toledo-Arruda

Silva

et al. 2022

Ther Adv Respir Dis

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Introduction: Although evidence suggests that physical exercise reduces systemic inflammation, at the plasma level, there are still contradictions in chronic obstructive pulmonary disease (COPD). In this sense, analysis of intracellular cytokines could clear off the effect of physical exercise on the inflammatory profile of these subjects. Aim: The aim was to evaluate the effect of physical training on cytokine expression in CD4+ T lymphocytes from subjects with COPD. Methods: This is a randomized controlled trial. Subjects with stable COPD were grouped into two groups, exercise and control. In total, 23 subjects with stable COPD were evaluated, of which 15 underwent aerobic strength training [physical exercise group (PEG)] and 8 underwent breathing exercises [respiratory physiotherapy group (RPG)]. Intracellular cytokines [interleukin (IL)-8, IL-13, IL-17, IL-6, IL-2, IL-10, and tumor necrosis factor alpha (TNF-α)] from CD4+ T lymphocytes were analyzed from peripheral blood through flow cytometry, before and after 8 weeks of intervention. Results: The PEG and RPG groups had a mean age of 68 ± 5.96 and 72.25 ± 6.86 years and predicted forced expiratory volume in the first second (FEV1) of 58.6 ± 15.99% and 39.75 ± 10.39%, respectively. It was possible to detect a significant reduction in IL-8 ( p = 0.0125) and an increase in IL-13 ( p = 0.0014) and an increase in TNF-α ( p < 0.001) in both groups. Conclusion: Eight weeks of physical training, both peripheral and respiratory, were able to reduce concentrations of IL-8 and to increase IL-13, and TNF-α in CD4+ T lymphocytes in subjects with stable COPD. The findings reinforce the benefits of interventions in subjects with COPD, revealing data not previously investigated.

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“…At the same time, there are publications indicating a low concentration of IL-10 in the blood in severe COPD [ 54 ]. Additionally, there is evidence of the lack of changes in IL-10 levels with the pathology progression [ 57 ]. These findings indicate the complex mechanisms of the participation of this cytokine in the pathogenesis of COPD.…”

Section: Discussionmentioning

confidence: 99%

Role of Toll-Like Receptor 2 in Regulation of T-Helper Immune Response in Chronic Obstructive Pulmonary Disease

Sidletskaya

Виткина

Denisenko

et al. 2021

Canadian Respiratory Journal

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Objective. According to modern views, the differences in the clinical course of chronic obstructive pulmonary disease (COPD) are associated with certain types of T-helper (Th) immune response. Recent data have shown that toll-like receptor 2 (TLR2) is involved in the development of Th immune response. However, TLR2-mediated regulation of Th subpopulation balance in COPD needs to be elucidated. The aim of our work is to determine the mechanisms of TLR2-mediated regulation of Th immune response in COPD of varying severity. Methods. The study included 323 smokers/ex-smokers with stable COPD (GOLD I, GOLD II, and GOLD III) and 97 healthy nonsmokers (control group). Serum levels of Th1 (TNF- α and IFN- γ ), Th2 (IL-4), Th17 (IL-6 and IL-17A), Treg (IL-10) cytokines, and the percentage of peripheral blood Th cells expressing TLR2 (CD4+CD282+) were assessed by flow cytometry. Serum concentrations of IL-21 (Th17) and TGF-β1 (Treg) were measured using the ELISA method. The predominant Th cytokine profile in serum was determined by calculating the ratios between levels of Th1 and Th17 cytokines. Spearman’s correlation test was performed. Results. Patients with COPD GOLD II and III with Th1 and Th17 cytokine profiles exhibited an increase in the percentage of CD4+CD282+ cells compared to the control group. In COPD GOLD I–III, positive correlations between CD4+CD282+ cell frequency and Th17 cytokine levels (IL-6, IL-17A, and IL-21) were found. In COPD GOLD I, IL-10 concentration was negatively correlated with the percentages of studied cells; in COPD GOLD II, a positive correlation between these parameters was noted. Conclusions. Enhanced TLR2 expression on CD4+ cells shifts cytokine profile toward Th17 phenotype that plays a crucial role in COPD progression. The level of TLR2 expression on peripheral blood CD4+ cells may be considered as a biomarker for diagnosing and predicting the progression of COPD.

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<p>Systemic Cytokine Profiles of CD4+ T Lymphocytes Correlate with Clinical Features and Functional Status in Stable COPD</p>

Cited by 7 publications

References 45 publications

Adiponectin Increase in Patients Affected by Chronic Obstructive Pulmonary Disease with Overlap of Bronchiectasis

Adiponectin Increase in Patients Affected by Chronic Obstructive Pulmonary Disease with Overlap of Bronchiectasis

Effect of physical training on cytokine expression in CD4+ T lymphocytes in subjects with stable COPD

Role of Toll-Like Receptor 2 in Regulation of T-Helper Immune Response in Chronic Obstructive Pulmonary Disease

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