&lt;p&gt;Strategies to enhance efficacy of SPION-labeled stem cell homing by magnetic attraction: a systemic review with meta-analysis&lt;/p&gt;

Ahn, Ye Ji; Kong, Tae Hoon; Choi, Jin Woo; Yun, Wan Su; Key, Joe L.; Seo, Young Joon

doi:10.2147/ijn.s204910

Cited by 23 publications

(16 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…There are some mechanisms of the incorporation of nanoparticles, such as passive diffusion, and receptor, clatharin, and caveoline mediated endocytosis 23 . It is assumed that the nanoparticles are taken up into cells generally through clathrin-mediated or caveolin-mediated pathways due to their shape and size 24 . Transfection agents, such as Lipofectamine 25 , poly-l-lysine 26 , and protamine 27 , are often used to achieve intracellular uptake of contrast agents.…”

Section: Discussionmentioning

confidence: 99%

Magnetic resonance imaging of umbilical cord stem cells labeled with superparamagnetic iron oxide nanoparticles: effects of labelling and transplantation parameters

Ohki

Saito

Fukuchi

2020

Sci Rep

View full text Add to dashboard Cite

cell tracking with magnetic resonance imaging (MRi) is important for evaluating the biodistribution of transplanted cells. Umbilical cord-derived mesenchymal stem cells (UC-MSCs) have emerged as a promising therapeutic tool in regenerative medicine. We examined the Uc-MScs labeled with superparamagnetic (Spio) and ultrasmall superparamagnetic iron oxide (USpio) in terms of cell functioning and imaging efficiency in vitro and in vivo. The UC-MSCs were co-incubated with SPIO or USPIO at a concentration of 50 or 100 µg/mL of label. Viability and proliferation were assessed by Trypan blue dye exclusion and MTT assay, respectively. Differentiation (chondrogenesis, osteogenesis, and adipogenesis) was induced to examine the impact of labelling on stemness. for in vitro experiments, we used 7-T MRI to assess the T 2 values of phantoms containing various concentrations of cell suspensions. For in vivo experiments, nine neonatal rats were divided into the control, SPIO, and USPIO groups. The UC-MSCs were injected directly into the rat brains. MRI images were obtained immediately and at 7 and 14 days post injection. The UC-MSCs were successfully labeled with SPIO and USPIO after 24 h of incubation. Cell viability was not changed by labelling. Nevertheless, labelling with 100 µg/mL USPIO led to a significant decrease in proliferation. The capacity for differentiation into cartilage was influenced by 100 µg/mL of SPIO. MRI showed that labeled cells exhibited clear hypointense signals, unlike unlabeled control cells. In the USPIO-labeled cells, a significant (P < 0.05) decrease in t 2 values (= improved contrast) was observed when compared with the controls and between phantoms containing the fewest and the most cells (0.5 × 10 6 versus 2.0 × 10 6 cells/mL). In vivo, the labeled cells were discernible on T 2-weighted images at days 0, 7, and 14. The presence of SPIO and USPIO particles at day 14 was confirmed by Prussian blue staining. Microscopy also suggested that the regions occupied by the particles were not as large as the corresponding hypointense areas observed on MRI. Both labels were readily taken up by the UC-MSCs and identified well on MRI. While SPIO and USPIO provide improved results in MRI studies, care must be taken while labelling cells with high concentrations of these agents. Mesenchymal stem cells (MSCs), a kind of somatic stem cells, are isolated from the mesenchymal tissues, such as fat, bone, and cartilage. MSCs can differentiate into mesodermal cells 1 , endodermal 2 , and ectodermal cells 3. Because of their regenerative and immunoregulatory capacities 4 , MSCs are expected to be applied for the therapy of various diseases, such as osteogenesis imperfecta, ischemic stroke, and cardiac infarction. Bone marrow is the most common source of MSCs. However, collecting MSCs from the bone marrow is highly invasive, and the maximal life span of the cells is affected by the donor's age 5. Currently, an umbilical cord tissue called Wharton's jelly is attracting attention, as a promising source of stem cells becaus...

show abstract

Section: Discussionmentioning

confidence: 99%

Magnetic resonance imaging of umbilical cord stem cells labeled with superparamagnetic iron oxide nanoparticles: effects of labelling and transplantation parameters

Ohki

Saito

Fukuchi

2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…It would be pathbreaking to develop an efficient delivery system of an intravenous injection of stem cells for the application of treating human ICH. There have been several studies on the intravenous injection of mesenchymal stem cells labeled with superparamagnetic iron oxide nanoparticles (SPIONs) under external magnetic fields [30]. FION, which is a smaller, faster, and more powerful magnetized tagging material compared with SPION, could be a more efficient targeted delivery material for stem cell transplantation [17,31,32].…”

Section: Discussionmentioning

confidence: 99%

Targeted Delivery of Iron Oxide Nanoparticle-Loaded Human Embryonic Stem Cell-Derived Spherical Neural Masses for Treating Intracerebral Hemorrhage

Kang

Kim

et al. 2020

IJMS

View full text Add to dashboard Cite

This study evaluated the potential of iron oxide nanoparticle-loaded human embryonic stem cell (ESC)-derived spherical neural masses (SNMs) to improve the transportation of stem cells to the brain, ameliorate brain damage from intracerebral hemorrhage (ICH), and recover the functional status after ICH under an external magnetic field of a magnet attached to a helmet. At 24 h after induction of ICH, rats were randomly separated into three experimental groups: ICH with injection of phosphate-buffered saline (PBS group), ICH with intravenous injection of magnetosome-like ferrimagnetic iron oxide nanocubes (FION)-labeled SNMs (SNMs* group), and ICH with intravenous injection of FION-labeled SNMs followed by three days of external magnetic field exposure for targeted delivery by a magnet-embedded helmet (SNMs*+Helmet group). On day 3 after ICH induction, an increased Prussian blue-stained area and decreased swelling volume were observed in the SNMs*+Helmet group compared with that of the other groups. A significantly decreased recruitment of macrophages and neutrophils and a downregulation of pro-inflammatory cytokines followed by improved neurological function three days after ICH were observed in the SNMs*+Helmet group. Hemispheric atrophy at six weeks after ICH was significantly decreased in the SNMs*+Helmet group compared with that of the PBS group. In conclusion, we have developed a targeted delivery system using FION tagged to stem cells and a magnet-embedded helmet. The targeted delivery of SNMs might have the potential for developing novel therapeutic strategies for ICH.

show abstract

“…32 Increasing evidence shows that cell responses to Fe 3 O 4 nanoparticles have indeed been observed, although the inherent characteristics of Fe 3 O 4 nanoparticle-labeled MSCs have not been fully explored. 33 Therefore, the strategy of using Fe 3 O 4 nanoparticles to enhance stem cell migration capacity can improve the effectiveness of stem cell therapy. 34 In this study, we synthesized polydopamine-capped Fe 3 O 4 (Fe 3 O 4 @PDA) and explored the role of UCMSCs labeled with NPs in enhancing stem cell regeneration in disc degeneration (Fig.…”

Section: Introductionmentioning

confidence: 99%

Fe3O4@polydopamine Nanoparticle-Labeled Umbilical Cord Mesenchymal Stem Cells Arrest Intervertebral Disc Degeneration by Enhancing Cell Migration

Zhang

Ran

et al. 2021

Preprint

View full text Add to dashboard Cite

Cell therapies for intervertebral disc degeneration (IDD) are intended to replace lost intervertebral disc (IVD) cells. The key to this treatment is to promote the migration of transplanted cells to the lesion site. The purpose of this study was to evaluate the repair effect of umbilical cord mesenchymal stem cells (UCMSCs) labeled with Fe3O4@polydopamine nanoparticles (Fe3O4@PDA NPs) on rat caudal vertebra disc degeneration. We characterized UCMSCs labeled with Fe3O4@PDA NPs, analyzed the effects of nanoparticles on UCMSCs and evaluated UCMSCs labeled with Fe3O4@PDA NPs to repair IDD in vivo. We found that UCMSC Fe3O4@PDA NPs could enhanced the migration of UCMSCs by up-regulating the expression of CXC chemokine receptor type 4 (CXCR4) without effecting UCMSC functionality and the Fe3O4@PDA NPs-labeled UCMSC group had better disc height, better tissue morphology performance and a higher number of transplanted cells and induced notably better regeneration of IVD, evidenced by the higher expression of aggrecan, type II collagen, and Sox-9 and lower expression of Mmp-13, Tnf-α and Il-1β at both mRNA and protein levels than the unlabeled group. We demonstrated systemic delivery of UCMSCs labeled with Fe3O4@PDA NPs could be an appropriate protocol for accelerating and optimizing clinically applicable UCMSC treatment for IDD.

show abstract

<p>Strategies to enhance efficacy of SPION-labeled stem cell homing by magnetic attraction: a systemic review with meta-analysis</p>

Cited by 23 publications

References 55 publications

Magnetic resonance imaging of umbilical cord stem cells labeled with superparamagnetic iron oxide nanoparticles: effects of labelling and transplantation parameters

Magnetic resonance imaging of umbilical cord stem cells labeled with superparamagnetic iron oxide nanoparticles: effects of labelling and transplantation parameters

Targeted Delivery of Iron Oxide Nanoparticle-Loaded Human Embryonic Stem Cell-Derived Spherical Neural Masses for Treating Intracerebral Hemorrhage

Fe3O4@polydopamine Nanoparticle-Labeled Umbilical Cord Mesenchymal Stem Cells Arrest Intervertebral Disc Degeneration by Enhancing Cell Migration

Contact Info

Product

Resources

About