<p>Silencing Of hsa_circ_0008450 Represses Hepatocellular Carcinoma Progression Through Regulation Of microRNA-214-3p/EZH2 Axis</p>

Abstract

Circular RNA (circRNA) hsa_circ_0008450 has been shown to be up-regulated in hepatocellular carcinoma (HCC). However, the functional role of hsa_circ_0008450 and its molecular mechanism are still unknown. Patients and methods: We used qRT-PCR and Western blot to examine the expression levels of hsa_circ_0008450, microRNA-214-3p (miR-214-3p), and enhancer of zeste homolog 2 (EZH2) protein. CCK8 assay and wound healing assay were used to detect cell viability and cell migration capability. Cell apoptosis was assessed by flow cytometry. Luciferase reporter assay was used to explore the interaction among hsa_circ_0008450, miR-214-3p, and EZH2. Results: hsa_circ_0008450 was significantly increased in HCC tissues and cells. Furthermore, knockdown of hsa_circ_0008450 in HCC cells inhibited cell proliferation, invasion, and migration. Mechanically, hsa_circ_0008450 promoted the expression of EZH2 protein through sponging miR-214-3p. Knockdown of circ_0008450 suppressed tumorigenesis of HCC cells in vivo. Conclusion: Knockdown of hsa_circ_0008450 inhibits HCC progression by regulating miR-214-3p/EZH2 axis. This study suggests that hsa_circ_0008450 may serve as a novel target for the treatment of HCC.

Hypoxia-induced circular RNA hsa_circ_0008450 accelerates hepatocellular cancer progression via the miR-431/AKAP1 axis

“…Zhang et al (17) revealed that circ_0008450 downregulation induced cell apoptosis and repressed cell invasion, proliferation and migration in HCC cells. Moreover, Lin et al (33) indicated that circ_0008450 knockdown reversed invasion, proliferation and migration of HCC cells. Furthermore, circ_0008450 was revealed to be an oncogene in nasopharyngeal cancer (16).…”

“…These data indicated that circ_0008450 acted as a potential diagnostic biomarker for HCC and hypoxia-induced circ_0008450 served a cancerogenic role in HCC. It has been reported that circ_0008450 regulated tumor progression by acting as a sponge for miRNAs (16,33). For instance, hsa_circ_0008450 knockdown impeded the advancement of hepatocellular carcinoma by downregulating enhancer of zeste homolog 2 by sponging miR-214-3p (33).…”

“…It has been reported that circ_0008450 regulated tumor progression by acting as a sponge for miRNAs (16,33). For instance, hsa_circ_0008450 knockdown impeded the advancement of hepatocellular carcinoma by downregulating enhancer of zeste homolog 2 by sponging miR-214-3p (33). Furthermore, miR-431 was revealed to act as an anti-tumor gene in a variety of cancers.…”

Hsa_circ_0002320: a novel clinical biomarker for colorectal cancer prognosis

“…Hsa_circ_0008450 promoted levels of the enhancer of zeste homolog 2 (EZH2) protein. [ 26 ] These findings indicate that circRNAs may be involved in tumorigenesis and tumor development. However, the diagnostic value of circRNAs in malignant tumors requires further research.…”

The Research Progression and Clinical Significance of Circular RNAs in Head and Neck Cancers

“…Compared to miRNAs and lncRNAs, circRNAs have their special biogenesis, properties, and biological functions. Due to the stability and tissue specificity of circRNA, various circRNAs have been demonstrated to regulate and influence cancers such as hepatocellular cancer [ 8 ], breast cancer [ 9 ], and colorectal cancer [ 10 ]. Head and neck cancers are the sixth most popular cancer worldwide [ 11 ] that occur in the oral cavity, thyroid gland, larynx, pharynx, and other sites.…”