&lt;p&gt;Shelterin Complex at Telomeres: Implications in Ageing&lt;/p&gt;

Mir, Seyed Mostafa; Tehrani, Sadra Samavarchi; Goodarzi, Golnaz; Jamalpoor, Zahra; Asadi, Jahanbakhsh; Khelghati, Nafiseh; Qujeq, Durdi; Maniati, Mahmood

doi:10.2147/cia.s256425

Cited by 39 publications

(21 citation statements)

References 138 publications

(183 reference statements)

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“…HT-29 is a mutant for the p53 gene. Shelterins protect the chromosomes and prevent their ends but any kind of genotoxic stress or mutation can induce tumor [32]. The p53 protein is known for its tumor suppressor properties and plays a major role in the maintenance of genome integrity [33].…”

Section: Discussionmentioning

confidence: 99%

Gene Expression Analysis of Specific Genes Related to Genomic Stability in Colon Cancer Cell Lines - A Resource for Cancer Research

Alamro

AlMousa

Almusallam

et al. 2022

JPRI

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Appropriate cell models are the foundation of cancer research as all the cell lines are not the same in their ability to mimic primary tumor tissue. Molecular characteristics of various cell lines can help in selecting the right cell model for biomedical studies. This study describes the gene expression profile of nine genes, include the six-protein shelterin complex, tankyrases1and 2, and CTNNB1 in three different models of human colon cancer cell lines. Also, the relative telomere length in each cell line was evaluated. All genes' expression levels were analyzed using a real-time polymerase chain reaction. Nearly all test genes were significantly highly expressed in HT-29 as compared to HCT-116 and SW-620. SW-620 record a significantly increased expression of the CTNNB1 gene (P-value < 0.01) while most genes were poorly expressed in HCT-116. A positive correlation between CTNNB1 and hTERT expression was found in HCT-116 and SW-620 but not in HT-29. Telomere was found to be of the same length in all cell. Gene expression data presented in our results can assist in selecting appropriate cell lines in colon cancer research. Unique molecular properties of various cancer cell lines should be evaluated before selecting a cell line mmodelfor research purposes.

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Section: Discussionmentioning

confidence: 99%

Gene Expression Analysis of Specific Genes Related to Genomic Stability in Colon Cancer Cell Lines - A Resource for Cancer Research

Alamro

AlMousa

Almusallam

et al. 2022

JPRI

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“…TIN2 is encoded by TINF2 gene, we observed that at the mRNA levelthis gene was underexpressed in ICM patients. TIN2 is a key component in the correct assembly of the shelterin complex in telomeres, since it allows the union of TRF1 and TRF2 to the heterodimer formed by POT1/TPP1 [ 32 ]. We also observed underexpression of the TNKS and TNKS2 genes.…”

Section: Discussionmentioning

confidence: 99%

Relationships of Telomere Homeostasis with Oxidative Stress and Cardiac Dysfunction in Human Ischaemic Hearts

et al. 2021

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Although the roles of telomeres and oxidative stress in ischaemic cardiomyopathy (ICM) are known, mechanisms of telomere homeostasis and their relationship with oxidative stress are incompletely understood. We performed two RNA-seq analyses (mRNA n = 23; ncRNA n = 30) and protein validation on left ventricles of explanted hearts from ICM and control subjects. We observed dysregulation of the shelterin and cohesin complexes, which was related to an increase in the response to cellular oxidative stress. Moreover, we found alterations at mRNA level in the mechanisms of telomeric DNA repair. Specifically, increased RAD51D mRNA levels were correlated with left ventricular diameters. RAD51D protein levels were unaltered, however, and were inversely corelated with the miR-103a-3p upregulation. We also observed the overexpression of lncRNAs (TERRA and GUARDIN) involved in telomere protection in response to stress and alterations in their regulatory molecules. Expression of the TERRA transcription factor ATF7 was correlated with superoxide dismutase 1 expression and left ventricular diameters. The levels of GUARDIN and its transcription factor FOSL2 were correlated with those of catalase. Therefore, we showed specific alterations in the mechanisms of telomeric DNA repair and protection, and these alterations are related to an increase in the response mechanisms to oxidative stress and cardiac dysfunction in ICM.

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“…Telomeres are present at the end of the chromosomes, which are tandem repeats of TTAGGG and are guanine‐rich and protect chromosome from degradation, recombination and fusion. The maintenance of telomere length is regulated by a reverse transcription catalytic submit (TERT) and telomerase RNA component (TERC) as well as the protein complex Shelterin that it could protect the chromosome ends 56 . As is well known, telomere attrition could cause DNA damage response, which may result in apoptosis, 57 inflammation, 58 or cellular senescence 59 .…”

Section: Telomere Attritionmentioning

confidence: 99%

The mechanisms of vascular aging

Wang

Mao

2021

Aging Medicine

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Vascular senescence is one of the hotspots in current research. With global average life expectancy increasing, delaying or reducing aging and age‐related diseases has become a pressing issue for improving quality of life. Vascular senescence is an independent risk factor for age‐related cardiovascular diseases (CVD) and results in the deterioration of CVD. Nevertheless, the underlying mechanisms of the vascular senescence have not been expressly illustrated. In this review, we attempt to summarize the recent literature in the field and discuss the major mechanisms involved in vascular senescence. We also underline key molecular aspects of aging‐associated vascular dysfunction in the attempt to highlight potential innovative therapeutic targets to delay the onset of age‐related diseases.

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<p>Shelterin Complex at Telomeres: Implications in Ageing</p>

Cited by 39 publications

References 138 publications

Gene Expression Analysis of Specific Genes Related to Genomic Stability in Colon Cancer Cell Lines - A Resource for Cancer Research

Gene Expression Analysis of Specific Genes Related to Genomic Stability in Colon Cancer Cell Lines - A Resource for Cancer Research

Relationships of Telomere Homeostasis with Oxidative Stress and Cardiac Dysfunction in Human Ischaemic Hearts

The mechanisms of vascular aging

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