2019
DOI: 10.2147/cmar.s213769
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<p>Reciprocal Role Of DNA Methylation And Sp1 Binding In Ki-67 Gene Transcription</p>

Abstract: PurposeDNA methylation plays major regulatory roles in gene transcription. Our previous studies confirmed that Ki-67 promoter is hypomethylated and Sp1 is a transcriptional activator of Ki-67 gene in cancer cells. However, whether Sp1-mediated transcriptional activation of Ki-67 is related to its methylation has not been studied yet.Materials and methodsIn this study, we confirmed that methylated CpG binding protein 2 (MBD2) binding to methylated DNA hindered the binding of Sp1 to Ki-67 promoter and then repre… Show more

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Cited by 7 publications
(4 citation statements)
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“…The obtained products were visualized by agarose gel electrophoresis and GelRed (Vimed, Xzhou, China). All experiments were carried out in triplicate [ 35 ].…”
Section: Methodsmentioning
confidence: 99%
“…The obtained products were visualized by agarose gel electrophoresis and GelRed (Vimed, Xzhou, China). All experiments were carried out in triplicate [ 35 ].…”
Section: Methodsmentioning
confidence: 99%
“…It can be used to examine a wide range of experimental parameters (46). A number of proteins bound at the same genomic location can identify a multiprotein chromatin complex where several proteins work together to regulate gene transcription or chromatin configuration (47)(48)(49). In this study, ChIP was done, and qRT-PCR was used to detect the efficiency of immunoprecipitation.…”
Section: Discussionmentioning
confidence: 99%
“…The basic functions of SP1 include binding to basic helix-loop-helix (bHLH) transcription factors (Zheng et al, 2018 ), acting as a DNA transcription activator (Gilmour et al, 2019 ), combining with histone acetyltransferases/histone deacetylases (Li et al, 2019a ), and interacting with HMG frame structural domains (Kovacevic Grujicic et al, 2005 ) ( Table 1 ).…”
Section: Gene Structure Composition and Regulation Of Sp1mentioning
confidence: 99%