<p>Psoralen Suppresses Cisplatin-Mediated Resistance and Induces Apoptosis of Gastric Adenocarcinoma by Disruption of the miR196a-HOXB7-HER2 Axis</p>

Abstract

The present study aimed to investigate the impact of psoralen on miR-196a-5p expression and function, and to reveal the mechanism underlying miR-196a-5p-mediated inhibition and the reversal of cisplatin (DDP) resistance. Methods: Serum samples were collected from 50 patients with gastric cancer (GC), and the association between miR-196a-5p expression and the response to chemotherapy was assessed. A DDP-resistant GC cell line was also established to determine the effects of miR-196a-5p and psoralen on DDP resistance. MGC803 cells were transfected with miR-196a-5p mimic and inhibitor vectors for the overexpression and downregulation of miR-196a-5p, respectively. Results: Clinical data analysis showed that the lower expression levels of miR-196a-5p were significantly associated with chemoresistance in patients with GC. Upregulation of miR-196a-5p significantly enhanced the anti-proliferative effect, apoptosis and sensitivity to DDP by regulating the protein expression levels of HOXB7, HER2, Bcl-2 and G 1 /S-specific cyclin-D1 (CCND1). Furthermore, psoralen reversed miR-196a-5p-induced DDP resistance and reduced the expression levels of HOXB7, HER2, Bcl-2 and CCND1. Conclusion: miR-196a-5p may be a novel biomarker of chemotherapeutic success in patients with GC and may also influence the sensitivity of GC cells to DDP. Moreover, psoralen can increase chemotherapeutic sensitivity by upregulating miR-196a-5p and then downregulating HOXB7-HER2 signaling axis.

Psoralen alleviates high glucose-induced HK-2 cell injury by inhibition of Smad 2 signaling via upregulation of microRNA 874

“…**P < 0.01 compared with control group; ## P < 0.01 compared with HG group;^P < 0.05 compared with HG + psoralen group;^^P < 0.01 compared with HG + psoralen group downregulation of miR-488 [28]. In another study of Lei Jin et al, psoralen increased chemotherapeutic sensitivity in patients with gastric cancer through upregulating miR-196a-5p [29]. Moreover, Xinfeng Lin et al reported that psoralen diminished TNF-α-induced atrophy, cytotoxicity and apoptosis in C2C12 myoblasts through downregulating the expression of miR-675-5P [30].…”

“…If other miRNAs were involved in the regulation process of psoralen in DN remains to be investigated. When psoralen was used to treat other diseases, different miRNAs were involved [28][29][30]. According to a study of Yongquan Huang et al, psoralen improved the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) by the Fig.…”

“…According to a study of Yongquan Huang et al, psoralen improved the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) by the downregulation of miR-488 [ 28 ]. In another study of Lei Jin et al, psoralen increased chemotherapeutic sensitivity in patients with gastric cancer through upregulating miR-196a-5p [ 29 ]. Moreover, Xinfeng Lin et al reported that psoralen diminished TNF-α-induced atrophy, cytotoxicity and apoptosis in C2C12 myoblasts through downregulating the expression of miR-675-5P [ 30 ].…”

Psoralen alleviates high glucose-induced HK-2 cell injury by inhibition of Smad 2 signaling via upregulation of microRNA 874

“…When psoralen was used to treat other diseases, different miRNAs were involved [30][31][32]. According to a study of Yongquan Huang et al, psoralen improved the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) by the downregulation of miR-488 [30].…”

“…According to a study of Yongquan Huang et al, psoralen improved the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) by the downregulation of miR-488 [30]. In another study of Lei Jin et al, psoralen increased chemotherapeutic sensitivity in patients with gastric cancer through upregulating miR-196a-5p [31]. Moreover, Xinfeng Lin et al reported that psoralen diminished TNF-α-induced atrophy, cytotoxicity and apoptosis in C2C12 myoblasts through downregulating the expression of miR-675-5P [32].…”