Patient safety culture is a major aspect of a good Dental and Oral Hospital services. The purpose of this study was to determine the perception of patient safety in dentist, young dentists, medical staff in dental and oral hospital. This type of quantitative descriptive research with cross-sectional approach. Research site in the dental and oral hospital of the University of Muhammadiyah Semarang. Sample of 65 respondents consisting of dentists, young dentists, and medical staff used simple random sampling techniques. Data analysis using univariate is presented in the frequency distribution table. Patient safety culture instruments were taken from the AHRQ. Data analysis using univariate which is presented in the frequency distribution table. The results are Perception of Dentists, Young Dentists and Medical staff, the assessment of internal cooperation, poor (50.8%), Perception the assessment of managers, good (64.4%). Perception communication appraisal of work unit, good (50.8%), Perception frequency reports of wrong actions or events is not expected, good (53.8%). The perception of the assessment of the patient safety level, good (55.4%). The perception of hospital management overall assessment, poor (58.5%). The conclusion is culture of patient safety in Dental and Oral Hospital from the perception of Dentists and Medical Staff are good.Budaya keselamatan pasien merupakan aspek utama dalam pelayanan Rumah Sakit Gigi dan Mulut secara baik. Tujuan penelitian ini untuk mengetahui persepsi keselamatan pasien pada dokter gigi, dokter gigi muda, staf medis di rumah sakit gigi dan mulut. Jenis penelitian ini deskriptif kuantitatif dengan pendekatan Cross-Sectional. Lokasi penelitian dilakukan di Rumah Sakit Gigi dan Mulut Universitas Muhammadiyah Semarang. Sampel penelitian sebanyak 65 responden, terdiri dari, dokter gigi, dokter gigi muda, dan staf medis, dengan menggunakan teknik simple random sampling. Instrument budaya keselamatan pasien diambil dari AHRQ. Analisis data menggunakan univariat yang disajikan dalam tabel distribusi frekuensi. Berikut hasil penilaian instumen AHRQ menurut persepsi dokter gigi, persepsi dokter gigi muda, dan staf medis terhadap penilaian. Kerjasama internal kurang baik (49,2%), persepsi manajer terhadap penilaian baik (64,4%), persepsi penilaian komunikasi di unit kerja baik (50,8%), persepsi frekuensi pelaporan kejadian yang tidak diharapkan baik (53,6%), persepsi penilaian tingkat keselamatan pasien baik (55,4%), persepsi keseluruhan manajemen rumah sakit kurang baik (58,2%). Kesimpulan bahwa budaya keselamatan pasien di Rumah Sakit Gigi dan Mulut dari persepsi dokter gigi, dokter gigi muda, dan staf medis sudah baik.