<p>Overexpression of miR-664 is associated with poor overall survival and accelerates cell proliferation, migration and invasion in hepatocellular carcinoma</p>

Abstract

Introduction Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related death worldwide. This study aimed to investigate the expression patterns of microRNA-664 (miR-664) in HCC tissues and cells, and assess its clinical significance and functional role in HCC. Patients and methods One hundred and thirty-four paired HCC and non-cancerous tissues were collected from patients who underwent surgery in Qianfoshan Hospital affiliated to Shandong University (Shandong, China) between 2009 and 2012. Expression of miR-664 was measured by quantitative real-time polymerase chain reaction (qRT-PCR). Prognostic value of miR-664 in HCC was evaluated using Kaplan–Meier survival analysis and Cox regression analysis. Cell proliferation was analyzed using the CCK-8 assay, and cell migration and invasion of HCC cells was evaluated by the Transwell assay. Results Expression of miR-664 was significantly upregulated in HCC tissues and cells when compared with the normal controls (all P <0.05). MiR-664 expression was associated with lymph node metastasis, TNM stage and differentiation (all P <0.05) in the HCC patients. High miR-664 expression predicted poor overall survival (log-rank P =0.004) and acted as an independent prognostic factor (HR =1.945, 95% CI=1.078–3.508, P =0.027). According to cell experiments, the upregulation of miR-664 could promote, whereas the downregulation of miR-664 could inhibit proliferation, migration and invasion of HCC cells (all P <0.05). SIVA1 was predicted as a direct target gene of miR-664 in HCC. Conclusion All data indicated that overexpression of miR-664 is associated with poor prognosis of HCC patients, and may enhance tumor progression of HCC by targeting SIVA1. MiR-664 may be a candidate therapeutic target for HCC treatment.

MiR‐664‐3p suppresses osteoblast differentiation and impairs bone formation via targeting Smad4 and Osterix

“…Previous studies demonstrated that miR-664-3p was closely related to the occurrence and development of a variety of tumours. [24][25][26] However, there have been no studies on the potential role of miR-664-3p in bone metabolism. In addition, two key regulators of osteogenesis, including Smad4 and Osterix (Osx), are the potential targets of miR-664-3p.…”

Downregulation of miR-484 is associated with poor prognosis and tumor progression of gastric cancer

“…The modular and cellular factors that governing these processes remain unclear [21]. Multiple studies have reported that microRNAs could regulate target mRNAs and its abnormal expression was closely related to the migration, invasion, and proliferation of various cancers [10,[22][23][24]. For instance, miR-593 can inhibit the migration and invasion of non-small cell lung cancer cells by targeting SLUG-associated signaling pathways [25].…”

“…Meanwhile, miRNAs participate in the regulation of a variety of human proteins, which makes it easier to affect the genetic pathways [5]. Currently, miRNA has been reported to act as oncogenes or antioncogenes in the initiation and development of various cancers [6][7][8][9][10]. For example, miR-423-3p acts as an oncogene and promotes cell proliferation, migration, and invasion of lung cancer [11].…”

CAFs-derived small extracellular vesicles circN4BP2L2 promotes proliferation and metastasis of colorectal cancer via miR-664b-3p/HMGB3 pathway

“… 19 The miR-664b-3p was involved in cell progression of HCC. 20 , 21 Starbase prediction showed that the expression of miR-664b-3p was significantly down-regulated in colon adenocarcinoma. Previous study reported that miR-664b-3p overexpression inhibited the proliferation and invasion of HCC cells.…”