&lt;p&gt;Multiple Components Rapidly Screened from Perilla Leaves Attenuate Asthma Airway Inflammation by Synergistic Targeting on Syk&lt;/p&gt;

Yang, Hui; Sun, Wei; Ma, Pei; Yao, Chun‐Suo; Fan, Yannan; Li, Shuyi; Yuan, Jing; Zhang, Ziqian; Li, Xuyu; Lin, Mingbao; Hou, Qi

doi:10.2147/jir.s281393

Cited by 14 publications

(18 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Experienced blinded pathologists performed the histopathological studies, according to a four-point scoring scale. 11 …”

Section: Methodsmentioning

confidence: 99%

“…[7][8][9] Syk has been proved to be a potential therapeutic target for both asthma and COPD inflammation. 10,11 In asthma, Syk is rapidly recruited and activated by FcεRI-mediated signaling pathways in human basophils, leading to cell degranulation and histamine release. 12 And in patients with COPD, Syk is one of the kinases required for neutrophils to form neutrophil extracellular traps (NET).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Syk-Targeted, a New 3-Arylbenzofuran Derivative EAPP-2 Blocks Airway Inflammation of Asthma–COPD Overlap in vivo and in vitro

Yang

Yuan

et al. 2021

JIR

Self Cite

View full text Add to dashboard Cite

Introduction: Asthma-chronic obstructive pulmonary (COPD) overlap (ACO) coexists with asthma and COPD syndrome characteristics, with more frequent exacerbations, heavier disease burden, higher medical utilization, and even lower quality of life. However, the ACO standard medications supported by evidence-based medicine have not yet appeared. Methods: By using an ACO mouse model established previously and LPS-stimulated RAW264.7 macrophages in vitro, a potential therapeutic candidate, EAPP-2, was screened from derivatives of 3-arylbenzofuran, and its effect and mechanism on ACO inflammation were evaluated. Results: EAPP-2 significantly alleviated airway inflammation in ACO mice and also inhibited the inflammatory reactions in LPS-induced RAW264.7 macrophages in vitro. Furthermore, EAPP-2 significantly inhibited the expression and phosphorylation of spleen tyrosine kinase (Syk), a common target regulating both eosinophils and neutrophils inflammation. In addition to this, EAPP-2 significantly down-regulates the expression of NF-κB, p-NF-κB, and NLRP3 in vivo and in vitro. Moreover, by using specific inhibitors in vitro, it was validated that EAPP-2 targeted on Syk and then regulated its downstream NF-κB and NLRP3. Conclusion: EAPP-2 is shown to be a potentially useful therapeutic candidate for ACO, and its mechanism is at least partially achieved by targeting on Syk and then inhibiting NF-κB or NLRP3. Moreover, this study suggests that Syk may be a potentially effective target for ACO therapy.

show abstract

“…Experienced blinded pathologists performed the histopathological studies, according to a four-point scoring scale. 11 …”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Syk-Targeted, a New 3-Arylbenzofuran Derivative EAPP-2 Blocks Airway Inflammation of Asthma–COPD Overlap in vivo and in vitro

Yang

Yuan

et al. 2021

JIR

Self Cite

View full text Add to dashboard Cite

show abstract

“…Treatment. The OVA-induced asthma mouse model was established according to a previously described method [17]. Briefly, animals were divided randomly into 6 groups: control (Con) group, model (Mod) group, PLE 100 mg/kg group, PLE 200 mg/kg group, PLE 400 mg/kg group, and Dex 0.5 mg/kg [18] group.…”

Section: Ova-induced Allergic Airway Inflammation Andmentioning

confidence: 99%

“…Analysis of Lung Tissues. Mouse lung tissues were fixed, paraffin-embedded, cut, and stained with hematoxylin-eosin (H&E) for analysis of inflammatory cell infiltration as described previously [17]. In another experiment, lung sections were dewaxed and rehydrated.…”

Section: Histologic Assessment and Immunohistochemicalmentioning

confidence: 99%

See 1 more Smart Citation

Perilla Leaf Extract Attenuates Asthma Airway Inflammation by Blocking the Syk Pathway

Yang

Sun

Fan

et al. 2021

Mediators of Inflammation

Self Cite

View full text Add to dashboard Cite

Perilla frutescens (L.) Britton is a classic herbal plant used widely against asthma in China. But its mechanism of beneficial effect remains undermined. In the study, the antiallergic asthma effects of Perilla leaf extract (PLE) were investigated, and the underlying mechanism was also explored. Results showed that PLE treatment significantly attenuated airway inflammation in OVA-induced asthma mice, by ameliorating lung pathological changes, inhibiting recruitment of inflammatory cells in lung tissues and bronchoalveolar lavage fluid (BALF), decreasing the production of inflammatory cytokines in the BALF, and reducing the level of immunoglobulin in serum. PLE treatment suppressed inflammatory response in antigen-induced rat basophilic leukemia 2H3 (RBL-2H3) cells as well as in OVA-induced human peripheral blood mononuclear cells (PBMCs). Furthermore, PLE markedly inhibited the expression and phosphorylation of Syk, NF-κB, PKC, and cPLA2 both in vivo and in vitro. By cotreating with inhibitors (BAY61-3606, Rottlerin, BAY11-7082, and arachidonyl trifluoromethyl ketone) in vitro, results revealed that PLE’s antiallergic inflammatory effects were associated with the inhibition of Syk and its downstream signals NF-κB, PKC, and cPLA2. Collectively, the present results suggested that PLE could attenuate allergic inflammation, and its mechanism might be partly mediated through inhibiting the Syk pathway.

show abstract

Perilla frutescens: A traditional medicine and food homologous plant

Dong

Chen

et al. 2023

Chinese Herbal Medicines

View full text Add to dashboard Cite

<p>Multiple Components Rapidly Screened from Perilla Leaves Attenuate Asthma Airway Inflammation by Synergistic Targeting on Syk</p>

Cited by 14 publications

References 36 publications

Syk-Targeted, a New 3-Arylbenzofuran Derivative EAPP-2 Blocks Airway Inflammation of Asthma–COPD Overlap in vivo and in vitro

Syk-Targeted, a New 3-Arylbenzofuran Derivative EAPP-2 Blocks Airway Inflammation of Asthma–COPD Overlap in vivo and in vitro

Perilla Leaf Extract Attenuates Asthma Airway Inflammation by Blocking the Syk Pathway

Perilla frutescens: A traditional medicine and food homologous plant

Contact Info

Product

Resources

About