<p>Multi-Layered Polyamide/Collagen Scaffolds with Topical Sustained Release of N-Acetylcysteine for Promoting Wound Healing</p>

Abstract

Background: Impaired wound healing might be associated with many issues, especially overactive of reactive oxygen species (ROS), deficiency of blood vessels and immature of epidermis. N-acetylcysteine (NAC), as an antioxidant, could solve these problems by inhibiting overreactive of ROS, promoting revascularization and accelerating re-epithelialization. How to deliver NAC in situ with a controllable releasing speed still remain a challenge. Materials and Methods: In this study, we combined collagen (Col) with N-acetylcysteine to perform the characteristics of sustained release and chemically crosslinked Col/NAC composite with polyamide (PA) nanofibers to enhance the mechanical property of collagen and fabricated this multi-layered scaffold (PA-Col/NAC scaffold). The physical properties of the scaffolds such as surface characteristics, water absorption and tensile modulus were tested. Meanwhile, the ability to promote wound healing in vitro and in vivo were investigated. Results: These scaffolds were porous and performed great water absorption. The PA-Col /NAC scaffold could sustainably release NAC for at least 14 days. After cell implantation, PA-Col/NAC scaffold showed better cell proliferation and cell migration than the other groups. In vivo, PA-Col/NAC scaffolds could promote wound healing best among all the groups. Conclusion: The multi-layered scaffolds could obviously accelerate the process of wound healing and exert better and prolonged effects.

Improvement of ECM-based bioroot regeneration via N-acetylcysteine-induced antioxidative effects

“…N -Acetylcysteine (NAC) is a thiol compound with a small molecular weight, and it is a precursor of glutathione (GSH) [ 22 , 23 ]. As an ROS scavenger, NAC regulates excessive inflammatory responses and prevents the oxidative stress triggered by ROS [ 24 ]. In addition, accumulating evidence has revealed that NAC exerts a wide range of pharmacological effects, such as induction of differentiation, antibacterial activity, and promotion of wound healing and osteogenic differentiation [ 5 , 22 , 25 ].…”

The Role of Antioxidants on Wound Healing: A Review of the Current Evidence

“…In the case of polyamide (PA) [52], the percentage of wound closure for PA-Col/NAC (69.3% ±11.47%) was higher than that of PA (37.95%±10.36%) and PA-Col (54.18%±12.51%) on day 7. On day 14, all the rats in the PA-Col/NAC group presented almost complete wound healing (86.17%±5.46%), while the percentage of wound closure for PA (55.15%±12.24%) and PA-Col (73.5%±7.46%) were much lower.…”

“…Moreover, Hou et al [51,52] developed a novel structured for wound healing, which was composed of nanofibers of biodegradable polycaprolactone (PCL) or polyamide (PA) polymers, N-acetyl cysteine (NAC), and collagen (Col). In vitro studies have demonstrated that the structure has good hygroscopicity, large porosity, and great biocompatibility.…”

Graphene Oxide Functionalized Double-Layered Patch with Anti-Adhesion Ability for Abdominal Wall Defects

“…NAC is approved by the FDA for clinical use as an antioxidant which has carboxyl thiol groups. 15 In recent years, more and more studies have suggested that NAC has the role of promoting neovascularization, and can reduce the levels of reactive oxygen species (ROS) at the point of injury area. 16 , 17 Moreover, in theory, the carboxyl group of NAC can form an amide bond with the amino group of CS under the activation of the EDC/NHS system to achieve the purpose of drug loading.…”