<p>MiR-935 Promotes Clear Cell Renal Cell Carcinoma Migration and Invasion by Targeting IREB2</p>

Abstract

Purpose: Clear cell renal cell carcinoma (ccRCC) has the highest rate of metastasis and invasion in RCC and is the third most common adult urinary malignancy. miRNA may serve a critical role in human cancer development and progression, has been confirmed to play a pivotal role in RCC cell invasion and migration. Since miR-935 had been verified to be an oncogene or tumor suppressor in various cancers, the role of miR-935 in RCC was unclear. Methods: Real-time quantitative polymerase chain reaction (qRT-PCR) was used to verify miR-935 expression. CCK-8 assay, wound healing assay and transwell assay were used to investigate the cell proliferation, migration and invasion of miR-935. Receiver operating characteristic (ROC) curve analysis was applied to discriminate different clinical classifications. Gain or loss of function approaches were used to investigate the cell proliferation, migration and invasion of miR-935 in vitro. Bioinformatics analysis and dual-luciferase reporter assay were used to identify the target of miR-935. Results: MiR-935 had a higher expression level in RCC cells and cancer tissues. MiR-935 mimics promoted cell proliferation, migration and invasion, and miR-935 inhibitor inhibited cell inhibit malignancy of cancer cells. Bioinformatics analysis and dual-luciferase reporter assay identified iron-responsive element-binding protein 2 (IREB2) as a direct target of miR-935. qRT-PCR showed IREB2 expression was downregulated in ccRCC cancer tissues and high IREB2 expression had a longer overall survival (OS) and disease-free survival (DFS). Silencing IREB2 could reverse the function of miR-935 inhibitor on cell proliferation and metastasis in renal cancer cells. Conclusion: The study indicated that miR-935 may act as an oncomiRNA and influenced migration and invasion progress of ccRCC by targeting IREB2. Oncogene miR-935 may be a molecular marker and uncover new strategies for ccRCC.

Regulation of ferroptosis by non‑coding RNAs in the development and treatment of cancer (Review)

“…Furthermore, miR-29a-related genetic variants alter the expression of IREB2 and may modify the risk of lung cancer together with dietary iron intake ( 79 ). Oncogenic miR-935 is elevated in renal cell carcinoma, and miR-935 directly suppresses the transcription of IREB2 by binding to the 3′-UTRs of IREB2 ( 80 ). Therefore, these miRNAs may suppress ferroptosis by targeting IREB2.…”

A novel circRNA, circNUP98, a potential biomarker, acted as an oncogene via the miR‐567/ PRDX3 axis in renal cell carcinoma

“…Amounting evidence suggested that miRNAs were involved in the tumorigenesis and progression of RCC 10 . For instance, it was found that miR‐935 promoted the migration and invasion of RCC cells via regulation of IREB2 11 . In contrast, miR‐765 functioned as a tumour suppressor and inhibited formation of lipids in RCC through inhibition of PLP2 12 .…”

Retracted: miR‐30d‐5p suppresses proliferation and autophagy by targeting ATG5 in renal cell carcinoma

“…MicroRNAs (miRNAs/miRs), a group of small noncoding RNAs~22-24 nucleotides in length, have been widely reported to negatively regulate their target genes at the posttranscriptional level by binding to the 3 0 UTR of target mRNAs, which play an important regulatory role in diverse cellular processes, including proliferation, apoptosis and autophagy [5][6][7]. Accumulating evidence indicates that miRNAs are aberrantly expressed in the development and progression of cancers by acting as tumor suppressors or oncogenes [8][9][10]. Recently, miR-30d-5p has been demonstrated to participate in tumor development by functioning as a tumor suppressor.…”