<p>miR-373 promotes neuroblastoma cell proliferation, migration, and invasion by targeting SRCIN1</p>

Abstract

Introduction Previous studies have shown that miR-373 functions as either a tumor suppressor or an oncogene depending on which type of cancer it’s operating in. However, the functional role of miR-373 in neuroblastoma (NB) remains largely unclear. Methods Expression of miR-373 and SRC kinase signaling inhibitor 1 (SRCIN1) in 20 metastatic and 20 primary NB tissues was detected by quantitative real-time PCR (qRT-PCR) and Western blotting. MTT assay, flow cytometry analysis and transwell migration and invasion assays were performed to evaluate the influence of miR-373 inhibition on the growth, migration and invasion of NB cells, respectively. In vivo experiment was applied to determine the effect of miR-373 inhibition on tumor growth. Dual-luciferase reporter assay was used to confirm the interaction between miR-373 and SRCIN1. Results We observed a significant increase in the expression of miR-373 in metastatic NB samples compared with primary NB samples, and this was inversely correlated with SRCIN1 expression. Functional studies revealed that depletion of miR-373 inhibited in vitro NB cell growth, migration and invasion, and also suppressed tumor growth in an in vivo mouse model. Moreover, we identified that SRCIN1 was a direct and functional target gene of miR-373. Silencing of SRCIN1 partially rescued the antimiR-373-mediated inhibition of cell growth, migration and invasion. Conclusion The data from our study verified a potential oncogenic role of miR-373 in NB cells that occurs through direct targeting SRCIN1. The newly identified miR-373/SRCIN1 axis represents a new potential candidate for therapeutic intervention of malignant NB.

The Scaffold Protein p140Cap as a Molecular Hub for Limiting Cancer Progression: A New Paradigm in Neuroblastoma

“…Further evidence supporting the biological relevance of p140Cap in curbing NB aggressiveness was provided by the recent work of Yuan XL et al [52]. Yuan XL et al demonstrated that SRCIN1 is a direct target of the microRNA-373 (miR-373) and that their expression has a negative correlation in both NB human samples and cell lines.…”

Integrative Transcriptomic Network Analysis of Butyrate Treated Colorectal Cancer Cells

“…miR-542 is a tumor suppressor reported to impact the development and progression of cancers such as CRC, hepatocellular carcinoma and breast cancer, where it is typically downregulated [ 37 , 38 , 39 , 40 ]. The miR-542-target gene BIRC5 (survivin) is a well-studied member of the inhibitor of apoptosis (IAP) family that is highly expressed in several cancer types and is involved in the regulation of numerous cellular pathways such as apoptosis, cell cycle, proliferation, metastasis and invasion [ 41 , 42 ].…”

“…The miR-542-target gene BIRC5 (survivin) is a well-studied member of the inhibitor of apoptosis (IAP) family that is highly expressed in several cancer types and is involved in the regulation of numerous cellular pathways such as apoptosis, cell cycle, proliferation, metastasis and invasion [ 41 , 42 ]. BIRC5 was previously shown to be regulated by miR-542 in CRC [ 38 , 43 ]; moreover, miR-542 was found to decrease proliferation and induce apoptosis in CRC cell lines [ 37 , 39 ]. A direct interaction between miR-542 and the BIRC5 3′UTR in CRC cells has already been validated in vitro [ 36 ].…”

MicroRNAs Possibly Involved in the Development of Bone Metastasis in Clear-Cell Renal Cell Carcinoma

“…We have found that miR-542-5p was associated with longer TTBM and TTMOTB, inversely correlated with the mRNA expression of genes involved in BM (CD44, CXCR4, RUNX2 and TCF7) and positively correlated with the mRNA expression of genes protective against BM (SATB2 and SMAD2). miR-542-5p acts as a tumor suppressor in non-small cell lung cancer (NSCLC) [ 67 ], neuroblastoma [ 102 ], osteosarcoma [ 103 ], CRC [ 104 ], BC [ 105 ], and hepatocellular carcinoma (HCC) [ 106 ], though contradictory evidence exists [ 107 ]. A role in bone metabolism has been described for the 3p strand of the miR-542 duplex, as a suppressor of osteoblast cell proliferation and differentiation by targeting BMP7-signalling [ 66 ].…”