&lt;p&gt;MiR-27a-3p Regulated the Aggressive Phenotypes of Cervical Cancer by Targeting FBXW7&lt;/p&gt;

Ben, Wei; Zhang, Guangmei; Huang, Yangang; Sun, Yuhui

doi:10.2147/cmar.s234897

Cited by 14 publications

(13 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present research, the mir-27a-3p expression in degenerative NP tissue, and cell was obviously lower in comparison with the control group. A lot of reports showed that mir-27a-3p was higher expressed in a series of cancers and impede cell apoptosis [14][15][16]. Mir-27a-3p in glioma tissues was obviously expressed lower in comparison with the control group.…”

Section: Discussionmentioning

confidence: 99%

Role of MiR-27a-3p in Intervertebral Disc Degeneration through Targeting RASSF5 via MST1/LATS1 and RAS/RAC1 Signaling Pathway

Yuan

Zhou

et al. 2022

Journal of Healthcare Engineering

View full text Add to dashboard Cite

Background. The apoptosis of nucleus pulposus (NP) cells reduces the number of nucleus pulposus cells in intervertebral disc tissue, resulting in intervertebral disc degeneration (IDD). MicroRNAs (miRNAs) play an important regulatory role in abnormal cell proliferation and apoptosis. Methods. The miR-27a-3p expressions in degenerative NP tissue and cells were measured via qPCR. The impacts of miR-27a-3p on the proliferation and apoptosis of human NP cells were evaluated by flow cytometry assays, MTT assays, and western blot analyses. In addition, target scan and luciferase reporter assay were applied to confirm that RASSF5 was directly binding to miR-27a-3p. Western blot was applied to assess the relationship between miR-27a-3p, RASSF5 and MST1/LATS1, and RAS/RAC1 signaling pathway. Results. MiR-27a-3p was downregulated in degenerative NP tissues and cells by comparison with the control group. MiR-27a-3p overexpression enhanced cell proliferation and suppressed apoptosis of NP cells, while the above factors showed an opposite tendency after in the miR-27a-3p inhibitor group. The western blot experiment similarly suggested mir-27a-3p apparently downregulated apoptosis-related proteins (Bax and caspase-3) and upregulated antiapoptotic proteins (Bcl-2). In addition, RASSF5 was confirmed to be directly regulated by miR-27a-3p using the luciferase reporter assay. Overexpressed RASSF5 could reverse the effects caused by miR-27a-3p mimic. Finally, miR-27a-3p could downregulate RASSF5 and affected the MST1/LATS1 and RAS/RAC1 pathway. Conclusion. MiR-27a-3p may target RASSF5 and enhance cell proliferation and imped cell apoptosis of the nucleus pulposus cells via the MST1/LATS1 and RAS/RAC1 pathway, lessening the degeneration of intervertebral discs.

show abstract

Section: Discussionmentioning

confidence: 99%

Role of MiR-27a-3p in Intervertebral Disc Degeneration through Targeting RASSF5 via MST1/LATS1 and RAS/RAC1 Signaling Pathway

Yuan

Zhou

et al. 2022

Journal of Healthcare Engineering

View full text Add to dashboard Cite

show abstract

“…We also described herein that the miRN-27-a-3p showed a significant differential expression in tears from the POAG patients and the OHT participants. This miRNA has been implicated in cell proliferation and apoptosis in different types of cancer [ 43 , 44 ]. Overexpression of this miRNA promotes proliferation and inhibits apoptosis of cancer cells.…”

Section: Discussionmentioning

confidence: 99%

miRNAs and Genes Involved in the Interplay between Ocular Hypertension and Primary Open-Angle Glaucoma. Oxidative Stress, Inflammation, and Apoptosis Networks

Raga-Cervera

Bolarín

Millán

et al. 2021

JCM

View full text Add to dashboard Cite

Glaucoma has no cure and is a sight-threatening neurodegenerative disease affecting more than 100 million people worldwide, with primary open angle glaucoma (POAG) being the most globally prevalent glaucoma clinical type. Regulation of gene expression and gene networks, and its multifactorial pathways involved in glaucoma disease are landmarks for ophthalmic research. MicroRNAs (miRNAs/miRs) are small endogenous non-coding, single-stranded RNA molecules (18–22 nucleotides) that regulate gene expression. An analytical, observational, case-control study was performed in 42 patients of both sexes, aged 50 to 80 years, which were classified according to: (1) suffering from ocular hypertension (OHT) but no glaucomatous neurodegeneration (ND) such as the OHT group, or (2) have been diagnosed of POAG such as the POAG group. Participants were interviewed for obtaining sociodemographic and personal/familial records, clinically examined, and their tear samples were collected and frozen at 80 °C until processing for molecular-genetic assays. Tear RNA extraction, libraries construction, and next generation sequencing were performed. Here, we demonstrated, for the first time, the differential expression profiling of eight miRNAs when comparing tears from the OHT versus the POAG groups: the miR-26b-5p, miR-152-3p, miR-30e-5p, miR-125b-2-5p, miR-224-5p, miR-151a-3p, miR-1307-3p, and the miR-27a-3p. Gene information was set up from the DIANA-TarBase v7, DIANA-microT-CDS, and TargetScan v7.1 databases. To build a network of metabolic pathways, only genes appearing in at least four of the following databases: DisGeNet, GeneDistiller, MalaCards, OMIM PCAN, UniProt, and GO were considered. We propose miRNAs and their target genes/signaling pathways as candidates for a better understanding of the molecular-genetic bases of glaucoma and, in this way, to gain knowledge to achieve optimal diagnosis strategies for properly identifying HTO at higher risk of glaucoma ND. Further research is needed to validate these miRNAs to discern the potential role as biomarkers involved in oxidative stress, immune response, and apoptosis for the diagnosis and/or prognosis of OHT and the prevention of glaucoma ND.

show abstract

“…Apart from involvement in cancer, downregulation of FBXW7 by miR-322 demonstrates a possible curing method to protect myocardium from ischemia/reperfusion injury. Collectively, there are still other miRNAs, such as miR129 (61), miR-92a-3p (62), miR182 (63), miR-27a-3p (64,65), miR-212/132 (66), miR-223-3p (67), miR-103a-3p (68), miR-25 (69), miR−25−3p (70), miR-144 (71), miR-101 (72), miR-188-5p (73), and miR-500a-3p (74), functioning discrepantly to mediate different cell phenotypes but via the identical mechanism of targeting FBXW7.…”

Section: Microrna Regulation Of Fbxw7mentioning

confidence: 99%

Recent Insight on Regulations of FBXW7 and Its Role in Immunotherapy

Xing

Zhang

et al. 2022

Front. Oncol.

View full text Add to dashboard Cite

SCFFBXW7 E3 ubiquitin ligase complex is a crucial enzyme of the ubiquitin proteasome system that participates in variant activities of cell process, and its component FBXW7 (F-box and WD repeat domain–containing 7) is responsible for recognizing and binding to substrates. The expression of FBXW7 is controlled by multiple pathways at different levels. FBXW7 facilitates the maturity and function maintenance of immune cells via functioning as a mediator of ubiquitination-dependent degradation of substrate proteins. FBXW7 deficiency or mutation results in the growth disturbance and dysfunction of immune cell, leads to the resistance against immunotherapy, and participates in multiple illnesses. It is likely that FBXW7 coordinating with its regulators and substrates could offer potential targets to improve the sensitivity and effects of immunotherapy. Here, we review the mechanisms of the regulation on FBXW7 and its tumor suppression role in immune filed among various diseases (mostly cancers) to explore novel immune targets and treatments.

show abstract

<p>MiR-27a-3p Regulated the Aggressive Phenotypes of Cervical Cancer by Targeting FBXW7</p>

Cited by 14 publications

References 39 publications

Role of MiR-27a-3p in Intervertebral Disc Degeneration through Targeting RASSF5 via MST1/LATS1 and RAS/RAC1 Signaling Pathway

Role of MiR-27a-3p in Intervertebral Disc Degeneration through Targeting RASSF5 via MST1/LATS1 and RAS/RAC1 Signaling Pathway

miRNAs and Genes Involved in the Interplay between Ocular Hypertension and Primary Open-Angle Glaucoma. Oxidative Stress, Inflammation, and Apoptosis Networks

Recent Insight on Regulations of FBXW7 and Its Role in Immunotherapy

Contact Info

Product

Resources

About