&lt;p&gt;MiR-1224-5p Activates Autophagy, Cell Invasion and Inhibits Epithelial-to-Mesenchymal Transition in Osteosarcoma Cells by Directly Targeting PLK1 Through PI3K/AKT/mTOR Signaling Pathway&lt;/p&gt;

Jin, Bicheng; Jin, Dongfang; Zhuo, Zhaozhen; Zhang, Bo; Chen, Kun

doi:10.2147/ott.s274451

Cited by 24 publications

(14 citation statements)

References 43 publications

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“…Therefore, exploring the mechanism of OC hallmarks is essential for the new treatment strategies. In recent years, miRNAs have attracted attention of many scholars because of its importance in the early diagnosis, occurrence and development of tumors, and tumor cell invasion and migration [ 19 , 20 ].…”

Section: Discussionmentioning

confidence: 99%

miR-486 Promotes the Invasion and Cell Cycle Progression of Ovarian Cancer Cells by Targeting CADM1

Wang

et al. 2021

Analytical Cellular Pathology

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Objective. To explore the role and possible underlying mechanism of miR-486 in ovarian cancer (OC) cells. Methods. The expression of miR-486 and CADM1 was detected by qRT-PCR in OC tissues and adjacent nontumor tissues and OC cell lines. The dual-luciferase reporter gene system was used to determine the targeting relationship between miR-486 and CADM1. CCK-8, colony formation assay, Transwell, and flow cytometry were performed to detect cell proliferation, cell invasion, cell cycle progression, and the apoptotic cell death, respectively. Western blot was carried out to detect the expression of CADM1 protein and the proteins associated with cell cycle progression. Results. miR-486 was significantly upregulated in OC tissues and cells, while CADM1 expression was significantly downregulated. Dual-luciferase reporter assays further confirmed that CADM1 was a target gene of miR-486. Interference with miR-486 could inhibit the proliferation and invasion and promoted the apoptosis of SKOV3 cells. Knocking down both miR-486 and CADM1 significantly increased the SKOV3 cell proliferation, invasion, and the number of cells transitioning from the G0/G1 phase into the S phase of cell cycle and reduced the cellular apoptosis. Western blot analysis revealed that the expression of cell cycle progression-related proteins (CyclinD1, CyclinE, and CDK6) was significantly reduced, and the p21 expression was increased when interfering with both miR-486 and CADM1 expression. Conclusion. Our results suggested that miR-486 could act as a tumor promoter by targeting CADM1 and be a potential therapeutic target for the treatment of OC.

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Section: Discussionmentioning

confidence: 99%

miR-486 Promotes the Invasion and Cell Cycle Progression of Ovarian Cancer Cells by Targeting CADM1

Wang

et al. 2021

Analytical Cellular Pathology

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“…38 Previous studies confirm that hyperactivation of the AKT/mTOR signaling pathway contributes to tumorigenesis, and leads to aberrant tumor growth and invasion, especially in osteosarcoma. [39][40][41] As these two sig-naling pathways are suggested to have a pro-tumorigenic effect in osteosarcoma, 42,43 our results partly explain the molecular mechanism underlying the pro-tumor effect of CLTC. Taken together, these findings revealed that CLTC played a pro-tumor function by the TGF-β and AKT/mTOR signaling pathways.…”

Section: Discussionmentioning

confidence: 54%

Deregulation of CLTC interacts with TFG, facilitating osteosarcoma via the TGF‐beta and AKT/mTOR signaling pathways

Pan

Qin

et al. 2021

Clinical & Translational Med

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1. High CLTC expression is associated with worse clinical outcomes in osteosarcoma patients. 2. Inhibition of CLTC suppresses osteosarcoma growth both in vitro and in vivo.3. SP1 binds to the CLTC promoter to promote the transcriptional activity of CLTC in osteosarcoma. 4. CLTC-mediated oncogenic effects occurred through activation of the TGF-beta and AKT/mTOR signaling pathway in a TFG-dependent manner.

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“…Reduced miR-1224 was common in the muscular and skeletal system. For instance, miR-1224-5p was decreased in osteosarcoma (OS) tissues and cell lines by qRT-PCR ( 27 , 28 ).…”

Section: Mir-1224 Expression In Human Cancermentioning

confidence: 99%

The Role and Mechanism of microRNA-1224 in Human Cancer

Zhang

et al. 2022

Front. Oncol.

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microRNAs (miRNAs) are a type of small endogenous non-coding RNAs composed of 20-22 nucleotides, which can regulate the expression of a gene by targeting 3’ untranslated region (3’-UTR) of mRNA. Many studies have reported that miRNAs are involved in the occurrence and progression of human diseases, including malignant tumors. miR-1224 plays significant roles in different tumors, including tumor proliferation, metastasis, invasion, angiogenesis, biological metabolism, and drug resistance. Mostly, it serves as a tumor suppressor. With accumulating proofs of miR-1224, it can act as a potential bio-indicator in the diagnosis and prognosis of patients with cancer. In this article, we review the characteristics and research progress of miR-1224 and emphasize the regulation and function of miR-1224 in different cancer. Furthermore, we conclude the clinical implications of miR-1224. This review may provide new horizons for deeply understanding the role of miR-1224 as biomarkers and therapeutic targets in human cancer.

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<p>MiR-1224-5p Activates Autophagy, Cell Invasion and Inhibits Epithelial-to-Mesenchymal Transition in Osteosarcoma Cells by Directly Targeting PLK1 Through PI3K/AKT/mTOR Signaling Pathway</p>

Cited by 24 publications

References 43 publications

miR-486 Promotes the Invasion and Cell Cycle Progression of Ovarian Cancer Cells by Targeting CADM1

miR-486 Promotes the Invasion and Cell Cycle Progression of Ovarian Cancer Cells by Targeting CADM1

Deregulation of CLTC interacts with TFG, facilitating osteosarcoma via the TGF‐beta and AKT/mTOR signaling pathways

The Role and Mechanism of microRNA-1224 in Human Cancer

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