<p>microRNA-139-3p Inhibits Malignant Behaviors of Laryngeal Cancer Cells via the KDM5B/SOX2 Axis and the Wnt/β-Catenin Pathway</p>

Abstract

Background: Laryngeal cancer (LCA) is a common head and neck cancer. Lysine demethylase 5B (KDM5B) knockdown is expected as a new target for cancer prevention. We investigated the molecular mechanism of KDM5B in LCA. Materials and Methods: The levels of KDM5B, microRNA (miR)-139-3p and highmobility-group box 2 (SOX2) in LCA tissues and cells, normal tissues and cells were detected. The effect of KDM5B on LCA was evaluated. The upstream miR of KDM5B and the downstream gene and pathway of KDM5B were predicted and their effects on LCA were analyzed. The Wnt/β-catenin pathway-specific activator agonist was delivered into LCA cells expressing miR-139-3p mimic to evaluate the role of the Wnt/β-catenin pathway. Results: KDM5B was highly expressed in LCA, and inhibition of KDM5B suppressed LCA progression. miR-139-3p, downregulated in LCA tissues, was a regulatory miR of KDM5B. Overexpression of miR-139-3p significantly inhibited the malignant biological behaviors of LCA cells. KDM5B promoted SOX2 expression via histone demethylation. SOX2 was highly expressed in LCA, and overexpression of SOX2 promoted LCA progression by inducing the Wnt/β-catenin pathway. Activated Wnt/β-catenin pathway attenuated the inhibitory effect of miR-139-3p mimic on the malignant biological behaviors of LCA cells. Conclusion: miR-139-3p overexpression inhibited LCA development via regulating the KDM5B/SOX2 axis and inhibiting the Wnt/β-catenin pathway.

Sequencing and validation of exosomal miRNAs panel as novel plasma biomarkers for early diagnosis and prognosis prediction in laryngeal cancer

“…Hsa-miR-139-3p has been con rmed to be down regulated in LSCC [26,27], which inhibits LSCC development by targeting rab5a [28], KDM5B/SOX2 axis and Wnt/β-catenin pathway [29]. Based on the above data and our ndings, we have reason to believe that hsa-miR-139-3p can be used as a reliable marker for the diagnosis and prognosis of LSCC.…”

Regulation of Oncogenic Targets by the Tumor-Suppressive miR-139 Duplex (miR-139-5p and miR-139-3p) in Renal Cell Carcinoma

“…In contrast to miR-139-5p , few studies have evaluated the importance of miR-139-3p in cancer because it is a passenger strand. Recently, it was reported that miR-139-3p directly regulates KDM5B (lysine demethylase 5B), a key regulator of histone 3 lysine 4 demethylation in laryngeal squamous cell carcinoma [ 28 ]. Ectopic expression of miR-139-3p suppressed cancer cell malignant behavior by inhibiting the Wnt/β-catenin pathway [ 28 ].…”

“…Recently, it was reported that miR-139-3p directly regulates KDM5B (lysine demethylase 5B), a key regulator of histone 3 lysine 4 demethylation in laryngeal squamous cell carcinoma [ 28 ]. Ectopic expression of miR-139-3p suppressed cancer cell malignant behavior by inhibiting the Wnt/β-catenin pathway [ 28 ]. In the HeLa cervical cancer cell line, miR-139-3p was reduced upon increasing expression of circular RNA hsa_circ_0031288 [ 29 ].…”

Diagnostic and Prognostic Value of microRNAs in Patients with Laryngeal Cancer: A Systematic Review

“…Of those, 166 were excluded for describing HNSCC cases without focus on the larynx ( n = 28), for being exclusively in vitro studies ( n = 39), for having a number of patients less than 10 or for using patient data from public databases ( n = 50), for not quantifying miRNAs in patient samples ( n = 43) and for other reasons, such as for not having statistically significant results or wrong miRNA sequence ( n = 6). Finally, the review was performed on a total of 177 studies [ 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 …”