<p>Loss of miR-204-5p Promotes Tumor Proliferation, Migration, and Invasion Through Targeting YWHAZ/PI3K/AKT Pathway in Esophageal Squamous Cell Carcinoma</p>

Abstract

MicroRNAs dysregulation has been confirmed in multiple malignancies. This paper reported the molecular mechanism of miR-204-5p in esophageal squamous cell carcinoma (ESCC). Methods: miR-204-5p expression in 30 ESCC tumor tissues and 10 normal tissues was downloaded from RNA-seq data. ESCC tissues/normal tissues of 97 ESCC patients were collected. TE-1 and KYSE510 cells were transfected by miR-204-5p mimic, inhibitor, siYWHAZ or their corresponding controls. The phenotype of cells was detected by CCK-8 assay, transwell experiment, and flow cytometry. Luciferase reporter gene assay and RNAbinding protein immunoprecipitation (RIP) were performed to verify the targeting relationship between miR-204-5p and YWHAZ. miR-204-5p and YWHAZ expression in tissues/cells was detected by qRT-PCR and Western blot. Xenograft tumor experiment was performed. Results: miR-204-5p expression was declined in ESCC patients and cells, which was indicated the poor outcome of patients. Compared with siNC group, TE-1 cells in miR-204-5p inhibitor group had higher OD450 value, less cell percentage in G1 phase, and more cell percentage in S phase, lower apoptosis percentage, and higher migration and invasion cell numbers. Moreover, KYSE510 cells of miR-204-5p mimic group showed lower OD450 value, more cell percentage in G1 phase and less cell percentage in S phase, higher apoptosis percentage, and lower migration and invasion cell numbers than control. YWHAZ was directly inhibited by miR-204-5p. Relative to siNC group, TE-1 cells of miR-inhibitor group exhibited higher YWHAZ protein expression, higher OD450 value, less cell percentage in G1 phase and more cell percentage in S phase, lower apoptosis percentage, higher migration and invasion cell numbers, and higher p-PI3K/PI3K and p-AKT/AKT protein expression, while siYWHAZ rescued the effects of miR-inhibitor. miR-204-5p up-regulation inhibited ESCC growth in vivo. Conclusion: miR-204-5p inhibits ESCC progression by targeted inhibition of YWHAZ/ PI3K/AKT.

mir-204-5p Acts as a Tumor Suppressor by Targeting DNM2 in Osteosarcoma Cells

“…Similarly, research studies have shown that miR-204-5p in cells and patients with esophageal squamous cell carcinoma is lower than that in normal tissues and cells, and it has also shown that the continuous decline of miR-204-5p is bound up with unfavorable prognosis of sufferers [ 18 ].…”

microRNA-204 shuttled by mesenchymal stem cell-derived exosomes inhibits the migration and invasion of non-small-cell lung cancer cells via the KLF7/AKT/HIF-1α axis

“…A recent study has reported the interaction between AKT and HIFcould exert great effects on the progression of NSCLC [20]. Interestingly, miR-204-5p expression was reduced in esophageal squamous cell carcinoma, and miR-204-5p repressed the development of esophageal squamous cell carcinoma by blocking the PI3K/AKT pathway [21]. Thus, we reasoned that MSCs-derived exosomes containing miR-204 could restrain NSCLC cell malignant phenotype via the KLF7/HIF-axis.…”

miR-204-5p inhibits cell proliferation and induces cell apoptosis in esophageal squamous cell carcinoma by regulating Nestin

“…In addition, previous studies have indicated that miR-204 suppresses cell proliferation, apoptosis, invasion and epithelial-mesenchymal transition (EMT) in ESCC [25][26][27]. Based on these studies, miR-204-5p was selected for this study.…”