<p>Long Noncoding RNA LINC00265 Targets EGFR and Promotes Deterioration of Colorectal Cancer: A Comprehensive Study Based on Data Mining and in vitro Validation</p>

Abstract

BackgroundLncRNAs are found to be aberrantly expressed in human cancers and could function as potential oncogenes or tumor suppressor genes. LINC00265 is a newly discovered LncRNA and its function in colorectal cancer (CRC) remains unknown.MethodsComprehensive bioinformatics analysis were performed to investigate the expression, clinical significance and potential biologic functions of LINC00265 in CRC based on the data from the Cancer Genome Atlas (TCGA). To further investigate the potential role of LINC00265 in CRC, we knocked down the LINC00265 expression in HT29 cells. The cell proliferation, invasion, cycle distribution, and apoptosis were evaluated in control, NC and siRNA groups. Additionally, effect of LINC00265 on the expression of EGFR was also measured.ResultsThe expression level of LINC00265 is increased in CRC tissues. Elevated level of LINC00265 is correlated with lymph node metastases and advanced pathological stage. We obtained 269 LINC00265 related genes; the results of functional analysis of these genes revealed that LINC00265 might involve in carcinogenesis of CRC. In addition, further experiments indicated that LINC00265 knockdown impaired cell proliferation and invasion, promoted cell cycle distribution and apoptosis in HT29 cells. Moreover, Western blot analysis revealed that downregulation of LINC00265 suppressed the expression of EGFR.ConclusionOur results indicate that LINC00265 induces cell proliferation, migration and inhibits CRC cells apoptosis by targeting EGFR. LINC00265 could be served as a diagnostic factor and therapeutic target for CRC patients.

Long intergenic noncoding RNA00265 promotes proliferation of gastric cancer via the microRNA-144-3p/Chromobox 4 axis

“…As expected, our data showed that LINC00265 was upregulated in GC tissues and cell lines. Additionally, it was observed that LINC00265 knockdown significantly restricted GC cell proliferation in vitro, similar to the previous studies in CRC 38 , suggesting that LINC00265 may serve as an oncogene in GC.…”

“…It has been reported that LINC00265 was upregulated in CRC and played a key role in disease progression. For instance, Ge et al suggested that increased LINC00265 expression closely correlated with lymph node metastases and advanced pathological stage in CRC, and LINC00265 could promote HT29 cell proliferation and invasion by upregulating the expression of EGFR [ 37 ]. Sun et al also demonstrated that LINC00265 was upregulated in CRC and intimately correlated with poorer patient prognosis.…”

<p>Long Non-Coding RNA C1QTNF1 Antisense RNA 1 Upregulates Hexokinase 2 by Sponging microRNA-484 to Promote the Malignancy of Colorectal Cancer</p>

“…14 Increasing evidence has demonstrated that a number of lncRNAs are dysregulated in CRC, contributing to CRC genesis and progression. [15][16][17] LncRNAs can execute cancer-inhibiting or cancer-promoting activities during CRC oncogenesis and are directly involved in several malignant processes. 18,19 MicroRNAs (MiRNAs) are a family of short noncoding RNA molecules with a length of approximately 17-24 nucleotides.…”

Demethylzeylasteral inhibits cell proliferation and enhances cell chemosensitivity to 5-fluorouracil in Colorectal Cancer cells

“…We believed that these 15 genes were key points in the regulation of the whole PPI network. We found that some genes were related to the proliferation and apoptosis of Colorectal cancer, such as EGFR, CDK2, CCN2 and BCL2L1 23 - 26 , and some were related to the prediction of chemotherapy for colorectal cancer, such as EGFR, TYMS 27 , 28 . Others, such as MMP9 29 , were associated with the metastasis of colorectal cancer.…”