<p>Long Noncoding RNA HIF1A-AS2 Promotes Non-Small Cell Lung Cancer Progression by the miR-153-5p/S100A14 Axis</p>

Abstract

Background: Long noncoding RNA (lncRNA) plays a critical role in initiating lung cancer. This study aims to research the function and mechanism of lncRNA HIF1A-AS2 in regulating non-small cell lung cancer (NSCLC) progression. Methods: qRT-PCR was used to analyze gene expression. The CCK-8 assay was performed to detect cell proliferation. The Transwell assay was conducted to examine cell migration and invasion. A Caspase3 activity detection kit was utilized to analyze apoptosis. The luciferase reporter assay was carried out to research interactions of HIF1A-AS2, miR-153-5p and S100A14. Results: HIF1A-AS2 expression was raised in NSCLC tissues and cell lines. The HIF1A-AS2 level was increased in advanced NSCLC tumor tissues. High HIF1A-AS2 expression was related to poor prognosis. HIF1A-AS2 knockdown decreased proliferation, migration and invasion while promoting apoptosis. HIF1A-AS2 was the sponge for miR-153-5p, and miR-153-5p targeted S100A14. HIF1A-AS2 promoted S100A14 expression through regulating miR-153-5p. Conclusion: The HIF1A-AS2/miR-153-5p/S100A14 axis plays a crucial role in promoting NSCLC progression.

HIF-1α Is a Rational Target for Future Ovarian Cancer Therapies

“…Considering that HIF-1a-AS downregulates the expression of HIF-1a (135) and because HIF-1a plays a role in the progression of cancer, we speculate that SC-144 inhibits the proliferation of ovarian cancer, at least to some extent, via the HIF-1a-AS/HIF-1a axis. However, the function of HIF-1a-AS in malignant tumours is complicated (136,137), and data on the role of HIF-1a-AS in ovarian cancer have not been reported. Hence, future investigation into the function of the HIF-1a-AS/HIF-1a axis in ovarian cancer is recommended (Figure 4).…”

lncRNA HIF1A‑AS2: A potential oncogene in human cancers (Review)

“…Thus, understanding the molecular mechanism of NSCLC and identifying novel therapeutic targets is of great clinical significance. Zhang et al ( 54 ) reported elevated expression levels of HIF1A-AS2 in NSCLC tissues and cell lines, and this increased expression was associated with a poor prognosis. However, knockdown of HIF1A-AS2 resulted in decreased cell proliferation, migration and invasion, and an increased percentage of apoptotic cells.…”

“…Wu et al ( 14 ) reported that HIF1A-AS2 functions as a protein decoy to inhibit the transcription of PHLDA1 by binding to LSD1, a histone demethylase. Additionally, HIF1A-AS2 acts as a molecular sponge to bind miRNAs to further affect expression of other genes ( 23 , 40 , 50 , 54 , 56 ). Although significant achievements have been obtained with regard to understanding the role of HIF1A-AS2 in various types of cancer, further studies are still required with regard to its regulatory function, as lncRNAs often exhibit several complex regulatory functions/mechanisms.…”

Remote ischaemic perconditioning reduces the infarct volume and improves the neurological function of acute ischaemic stroke partially through the miR-153-5p/TLR4/p65/IkBa signalling pathway

“…Interestingly, when the brain is in a hypoxic and ischaemic environment, neurological impairment and neurodegenerative diseases will also have a close relationship with miRNA [4]. Several studies have reported that miR-153-5p has significant anti-tumour effects [7,23]. However, there is no report about the expression characteristic and potential value of miR-153-5p after ischaemic stroke.…”