<p>Long Noncoding RNA GAS5 Acts As A Tumor Suppressor In Laryngeal Squamous Cell Carcinoma Via miR-21</p>

Abstract

PurposeLong noncoding RNAs (lncRNAs) have been identified as an important class of noncoding RNAs that are deeply involved in multiple biological processes in tumorigenesis. This study is to investigate the critical roles and biological function of lncRNA growth arrest-specific 5 (GAS5) in tumorigenesis of laryngeal squamous cell carcinoma (LSCC).Patients and methodsA total of 59 samples of LSCC and paired adjacent tissue, as well as corresponding clinicopathological information were collected. GAS5 expression in both LSCC tissues and human SUN1076 and SNU899 cell lines were analyzed by Real-time quantitative RT-PCR method. Ectopic expression of GAS5 by vector transfection in LSCC cell lines and followed by in vitro experiments was to investigate the critical roles and function of GAS5 in LSCC. Cell Counting Kit 8 (CCK8) assay and PE/7AAD Annexin V Apoptosis analysis was to evaluate cell proliferation ability and cell apoptosis. Co-transfection of GAS5 and miR-21 was to explore the interaction between GAS5 and miR-21 in LSCC. BAX and CDK6 protein level were analyzed by western blot method.ResultsThis study demonstrated that GAS5 was significantly downregulated in LSCC tissue and human LSCC cell lines. GAS5 levels were correlated with the clinicopathological features of LSCC patients. In addition, the ectopic expression of GAS5 significantly inhibited cell proliferation and promoted apoptosis. Co-expression analyses indicated that GAS5 is negatively correlated with miR-21 in LSCC tissues. Overexpression of miR-21 eliminated GAS5-mediated cell apoptosis and proliferation suppression. Furthermore, GAS5, which upregulated BAX mRNA expression and downregulated CDK6 mRNA expression, was reversed by ectopic expression of miR-21.ConclusionGAS5 suppresses LSCC progression through the negative regulation of miR-21 and its targets involved in cell proliferation and apoptosis, indicating that GAS5 may serve as a biomarker and potential target for LSCC therapy.

The Long Noncoding RNA, Growth Arrest-Specific 5, Suppresses Gastric Cancer by Downregulating miR-21 Expression

“…miR-21 can downregulate the Cdc25A, change the negative regulation of the G1/S phase of the cell cycle, and involve in DNA damage repair G2/M check the 19 point [31]. Numerous researches have shown that LncRNA GAS5 acts as a tumor suppressor in many types of malignancies by regulating the miR-21 [32][33][34][35]. In our research, we found overexpression of GAS5 reduced the resistance of drug and increased the apoptosis of gastric cancer cells.…”

<p>Long Non-Coding RNA GAS5 Suppresses Tumor Progression and Enhances the Radiosensitivity of Prostate Cancer Through the miR-320a/RAB21 Axis</p>

“…30,31 It is worth noting that GAS5 is provably down-regulated in various cancers and has the potential to be a tumor inhibitor. [32][33][34] With one accord, our results proved the inhibition of GAS5 expression in PCa tissues and cells.…”

Low GAS5 expression may predict poor survival and cisplatin resistance in cervical cancer

“…In gastric cancer, lncRNA MT1JP regulated the expression of FBXW7 through competitively binding with miR-92a-3p, and played a role in inhibiting cell proliferation, migration, invasion and promoting cell apoptosis 50 . MiR-21 sponged by GAS5 has been reported in OSCC, hepatocellular carcinoma, laryngeal squamous cell carcinoma and ovarian cancer 13,[51][52][53] . In our study, we verified the same mechanism in CC cells.…”