<p>Long Noncoding RNA DIO3OS Hinders Cell Malignant Behaviors of Hepatocellular Carcinoma Cells Through the microRNA-328/Hhip Axis</p>

Abstract

Background: The decline of a long non-coding RNA (lncRNA) DIO3OS was implicated in a plethora of cancers, while the relevance in hepatocellular carcinoma (HCC) has not been mentioned. Accordingly, we set to determine the functional role of DIO3OS and the molecular mechanism in HCC progression. Materials and Methods: The differentially expressed lncRNAs, mRNAs, and microRNAs (miRNAs) were obtained through the datasets GSE101728 and GES57555. Afterwards, DIO3OS was enhanced in HCC cells to examine the behavior changes. Subcellular localization of DIO3OS was determined through website prediction and experimental validation. The expression of Hedgehog (Hh) signaling pathway-related genes was detected. The effects of DIO3OS overexpression on tumor growth were evaluated as well. Results: DIO3OS was lower in HCC tissues and cells, while upregulation of DIO3OS repressed malignant biological behavior both in vitro and in vivo. DIO3OS, localized in the cytoplasm, inhibited the occurrence of HCC by disrupting the Hh pathway by sponging miR-328 to mediate Hh interacting protein (Hhip). Conclusion:All in all, the obtained data suggested that DIO3OS interacted with Hhipdependent Hh signaling pathway to inhibit HCC progression through binding to miR-328, which may be a potent therapeutic target for HCC.

Integrated analysis of ceRNA network reveals potential prognostic Hint1-related lncRNAs involved in hepatocellular carcinoma progression

“…LINC00324, SNHG3, and DIO3OS were identified as survival-related hub lncRNAs. Among them, DIO3OS has been reported to inhibit the proliferation and invasion of HCC cells by competitive binding to miR-328 [36]; our results are consistent with that finding. In addition, Zhang et al [37] reported that SNHG3 can promote the EMT process of HCC cells through competitive binding of miR-128, and then release CD151.…”

Expression Profiles and Potential Functions of Long Non-Coding RNAs in the Heart of Mice With Coxsackie B3 Virus-Induced Myocarditis

“…Another study showed that DIO3OS exhibited oncogenic properties by stimulating pancreatic cancer cell proliferation and invasion and promoting cancer growth by binding to miRNA-122 ( Cui et al., 2019 ). Conversely, DIO3OS levels were lower in hepatocellular carcinoma tissues, and upregulation of DIO3OS repressed malignant biological behavior by sponging miR-328 ( Wang et al., 2020 ). Other studies have also shown that the expression levels of DIO3OS were significantly lower in patients with Crohn’s disease and ulcerative colitis than those in healthy controls.…”

Noncoding RNAs related to the hedgehog pathway in cancer: clinical implications and future perspectives

“…LncRNA LINC-PINT, as a tumor suppressor gene, targets miR-425-5p which targets Ptch1 of the Hh pathway to modulate laryngeal carcinoma cell stemness and chemoresistance. Several other regulatory networks comprising lncRNA, miRNA, and mRNA have been reported in the Hh-mediated cancer, including DIO3OS/miR-328/Hhip [ 139 ], GAS5/miR-378a-5p/Sufu [ 140 ], LIFR-AS1/miR-197-3p/Sufu [ 141 ], LOC101930370/miR-1471/Shh [ 142 ], TUG1/miR-132/Shh [ 143 ], LINC01510/miR-335/Shh [ 144 ], LINC01123/miR-516b-5p/Gli1 [ 145 ], NEAT1/miR-503/Smo [ 146 ] and MIRLET7BHG/miR-330-5p/Smo [ 147 ].…”