<p>LncRNA DANCR-miR-758-3p-PAX6 Molecular Network Regulates Apoptosis and Autophagy of Breast Cancer Cells</p>

Abstract

Objective: This study set out to probe into the effects of long non-coding RNA (LncRNA) differentiation antagonizing non-protein coding RNA (DANCR) on apoptosis and autophagy of breast cancer (BC) cells. Methods: The expression levels of DANCR, miR-758-3p and paired box 6 (PAX6) in BC tissues and cell lines were detected. The transcription and protein levels of PAX6, apoptosisrelated factors (caspase-3, caspase-9, Bax/Bcl-2), and autophagy-related factors (LC3B, Atg5, Beclin-1) in BC cells were detected. The cell proliferation, apoptosis, autophagy and the regulatory relationship between genes and target genes were analyzed. Results: DANCR and PAX6 were up-regulated in BC tissues and cell lines, while miR-758-3p was opposite. Down-regulating DANCR inhibited the malignant proliferation of BC cells and also promoted apoptosis and autophagy, which showed that caspase-3, caspase-9, Bax/ Bcl-2, LC3B, Atg5 transcription and protein levels increased, while Beclin-1 transcription and protein levels decreased. DANCR regulated miR-758-3p in a targeted manner, and its over-expression could weaken the anti-cancer effect of miR-758-3p on BC cells. In addition, miR-758-3p also directly targeted PAX6, and knocking down its expression could weaken the inhibitory effect of down-regulating PAK6 on BC cell apoptosis and autophagy. We also found that DANCR acted as a competitive endogenous RNA sponge miR-758-3p, thus regulating the PAX6 expression. Conclusion: DANCR-miR-758-3p-PAX6 molecular network plays a key regulatory role in BC cell apoptosis and autophagy, which may provide reference for treating patients.

<p>H19 Knockdown Suppresses Proliferation and Induces Apoptosis by Regulating miR-130a-3p/SATB1 in Breast Cancer Cells</p>

“…TP73-AS1-miR-200a, NEAT1-miR-211, and DANCR-miR-758-3p axes are closely related to BC progression. [23][24][25] A bioinformatics based analysis conducted by our study predicted that H19 contained binding sites for miR-130a-3p, and that miR-130a-3p expression was regulated by H19 in vitro. Further experimental verification showed that miR-130a-3p exerted antitumor effects on BC, which result substantiated those of previous studies regarding the role of miR-130a-3p in glioma.…”

Exploring the Mechanism of MicroRNA-125a Reversing the Resistance of Laryngeal Cancer Stem Cells to Cisplatin from Mitochondrial Autophagy

“…As a common cytotoxic anti-tumor drug, DDP has been used as the first-line chemotherapy drug for laryngeal cancer in the past decades [11] . DDP promotes apoptosis through DNA damage and changes in cell metabolism.…”

Long Non-Coding RNA SNHG19 Promotes Breast Cancer Progression by Regulating miR-299-5p

“…LINC01133, downregulated in breast cancer, restrained breast cancer invasion and metastasis via inhibiting SOX4 transcription through binding with EZH2 [3]. LncRNA DANCR exerted crucial regulatory role in apoptosis and autophagy of breast cancer cells through DANCR/miR-758-3p/PAX6 pathway [15]. LncRNA CBR3-AS1 regulated adriamycin resistance of breast cancer cells by sponging miR-25-3p and regulating MAPK signal pathway [16].…”