<p>Lamin B1 Overexpresses in Lung Adenocarcinoma and Promotes Proliferation in Lung Cancer Cells via AKT Pathway</p>

Abstract

These authors contributed equally to this workPurpose: This study aims to investigate the biological effect and molecular mechanism of Lamin B1(LMNB1) in lung cancer cells and its significance for the prognosis of lung adenocarcinoma(LUAD) patients. Methods: In this study, Bioinformatics was performed to analyze the expression at mRNA level and prognosis effect of LMNB1 in LUAD from TCGA dataset. The immunohistochemistry(IHC) assay was conducted to analyzed the expression of LMNB1 at the protein level in LUAD tissues. The correlation between the expression of LMNB1 and the clinical factors in patients with LUAD was analyzed. Next, LMNB1 transfected into LUAD cell lines (A549 and PC-9) which was proved by Western blot. CCK8 assay, cloning formation assay, and xenograft assay were conducted to explore the effect and mechanism of LMNB1 on the proliferation of LUAD cell lines in vitro and in vivo. Results:The results of the present study demonstrated that LMNB1 was highly expressed in LUAD tissues and related to tumor stage. High LMNB1 expression was related with more advanced clinicopathological factors such as low degree of differentiation (P=0.02), large tumor size (P<0.01), lymph node metastasis (P<0.01) and higher tumor stage (P<0.01). After knocking down LMNB1, the cell growth rate (P<0.01) and the number of colonies (P<0.01) were significantly reduced, and the level of the proliferating marker Ki67 (P<0.01) was significantly decreased. At the same time, in vivo experiments showed that the tumor volume and tumor of the mice were significantly reduced (P<0.01). Moreover, we found that knockdown LMNB1 can inhibit the proliferation of lung cancer cells by inhibiting AKT phosphorylation by Western blot. Conclusion: In summary, LMNB1 play an of vital roles in the growth of LUAD cells, highlighting its potential as a therapeutic target for the treatment of LUAD patients.

LMNB2 is a prognostic biomarker and correlated with immune infiltrates in hepatocellular carcinoma

“…7 Another study indicated that LMNB1 is significantly upregulated in lung adenocarcinoma tissues and regulates the proliferation of lung cancer cells through the AKT signaling pathway. 8 However, the biological role of LMNB2 in HCC has not been explored.…”

“…Meanwhile, elevated LMNB2 level has been associated with worse histological grade, advanced tumor TNM stage, and poor prognosis in non‐small cell lung cancer (NSCLC) 7 . Another study indicated that LMNB1 is significantly upregulated in lung adenocarcinoma tissues and regulates the proliferation of lung cancer cells through the AKT signaling pathway 8 . However, the biological role of LMNB2 in HCC has not been explored.…”

Pan-cancer Analysis Identifies LMNB1 as a Target to Redress Th1/Th2 Imbalance and Enhance PARP Inhibitor Response in Human Cancers

“…The role of LMNB1 in tumor initiation and progression still remain elusive. Based on the data of TCGA, we found that LMNB1 was highly expressed in not only lung adenocarcinoma (11), prostate cancer (12,13), cervical cancer (14) and liver cancer ( 16), but also most other human cancer types. Though previous reports indicated that LMNB1 was downregulated in breast cancer by qRT-PCR (17), gastric cancer (18), lung adenocarcinoma and squamous cell carcinoma by IHC staining (19), TCGA analysis indicated LMNB1 expression in the above 4 tumor kinds was upregulated at least in the mRNA level.…”

“…Lamins are found implicated in the regulation of in a wide variety of nuclear processes, including DNA replication and repair, mitotic spindle assembly, chromosome distribution, oxidative stress response, and gene expression (5)(6)(7)(8)(9)(10). Recently, increased studies have revealed that LMNB1 is abnormally expressed in a lot of human cancer types, such as upregulated in lung adenocarcinoma (11), prostate cancer (12,13), cervical cancer (14), pancreatic cancer (15), liver cancer (16), and downregulated in breast cancer (17), gastric cancer (18) and two subtypes of lung cancer (19). Though previous evidences showed that LMNB1 expression affected the clinical behavior and patient outcome of the above cancer types, the precise mechanism underlying carcinogenesis of LMNB1 has not been extensively explored.…”

Pan-cancer analysis identifies LMNB1 as a target to redress Th1/Th2 imbalance and enhance PARP inhibitor response in human cancers

“…Lamins are found implicated in the regulation of in a wide variety of nuclear processes, including DNA replication and repair, mitotic spindle assembly, chromosome distribution, oxidative stress response, and gene expression [ 5 – 10 ]. Recently, increased studies have revealed that LMNB1 is abnormally expressed in a lot of human cancer types, such as upregulated in lung adenocarcinoma [ 11 ], prostate cancer [ 12 , 13 ], cervical cancer [ 14 ], pancreatic cancer [ 15 ], liver cancer [ 16 ], and downregulated in breast cancer [ 17 ], gastric cancer [ 18 ] and two subtypes of lung cancer [ 19 ]. Though previous evidences showed that LMNB1 expression affected the clinical behavior and patient outcome of the above cancer types, the precise mechanism underlying carcinogenesis of LMNB1 has not been extensively explored.…”