<p>Knockdown of <em>LINC02471</em> Inhibits Papillary Thyroid Carcinoma Cell Invasion and Metastasis by Targeting miR-375</p>

Abstract

Background: LncRNAs play important roles in papillary thyroid carcinoma (PTC). LINC02471 has been reported to be related to PTC prognosis. The current study aimed to investigate the effects of LINC02471 on human PTC cells. Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to examine LINC02471 expression in PTC tissues and cells and miR-375 expression in PTC cells. SiLINC02471, miR-375 mimic and miR-375 inhibitor were used for cell transfection. Cell proliferation, apoptosis, migration, and invasion were detected by performing Cell Counting Kit-8 (CCK-8), clone formation assay, flow cytometry, scratch assay, and transwell assay. Western blot was carried out to detect protein levels of E-cadherin, N-cadherin and Snail. The target gene for LINC02471 was verified by dual-luciferase reporter assay. Results: LINC02471 was highly expressed in PTC tissues and cells. After silencing LINC02471, cell proliferation, migration and invasion were reduced, but cell apoptosis was increased. SiLINC02471 increased the expressions of E-cadherin and miR-375, and inhibited the expressions of N-Cadherin and Snail. LINC02471 directly targeted miR-375 in PTC cells. Overexpression of miR-375 inhibited the proliferation, migration, invasion of PTC cells and reduced the expressions of N-Cadherin and Snail but promoted the cell apoptosis and increased E-cadherin expression, while miR-375 inhibitor produced opposite effects to overexpressed miR-375. After inhibiting miR-375 expression, siLINC02471 reversed the effect of miR-375 inhibitor. Conclusion: LINC02471 could promote the development of PTC. Knocking down LINC02471 could inhibit invasion and metastasis and promote PTC cell apoptosis through directly targeting miR-375.

Development and Interpretation of a Genomic Instability Derived lncRNAs Based Risk Signature as a Predictor of Prognosis for Clear Cell Renal Cell Carcinoma Patients

“…LINC02471 has been reported as a risk factor in thyroid carcinoma. The knockdown of LINC02471 increases the expression of miR-375, which would inhibit the proliferation and invasion and promote the apoptosis of tumor cells (43). The function of LINC01606 has been reported in GC.…”

A Radioresponse-Related lncRNA Biomarker Signature for Risk Classification and Prognosis Prediction in Non-Small-Cell Lung Cancer

“…LINC02471, a member of the intergenic lncRNA family, is recognized as a crucial enhancer in papillary thyroid carcinoma (PTC). Chen et al observed that downregulation of LINC02471 could hinder PTC cell metastasis and trigger l apoptosis by binding with miR-375, indicating that it can be considered as a potent predictor for metastasis of patients with PTC [ 49 ]. MAGI2-AS3 was originally identified as an antisense RNA of Membrane Associated Guanylate Kinase 2 (MAGI2) involved in regulating the malignant behaviors of various types of cancers.…”

An Autophagy-related lncRNA Prognostic Risk Model for Thyroid Cancer

“…Chen et al demonstrated that LINC02471 can promote the development of PTC. Knockdown of LINC02471 can also inhibit the invasion and metastasis of PTC, and promote apoptosis of PTC cells by directly targeting miR-375 [33]. Cal et al reported that LINC02471 can be used as a molecular biomarker for the progression and prognosis of thyroid cancer [34].…”