&lt;p&gt;Investigation of the prevalence of genes conferring resistance to carbapenems in &lt;em&gt;Pseudomonas aeruginosa&lt;/em&gt; isolates from burn patients&lt;/p&gt;

Khosravi, Azar Dokht; Taee, Shahab; Dezfuli, Aram Asarehzadegan; Meghdadi, Hossein; Shafie, Fatemeh

doi:10.2147/idr.s197752

Cited by 18 publications

(21 citation statements)

References 22 publications

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“…25 Yet, several surveillance programs have reported a highly increasing carbapenem resistance, making clinical treatment more challenging. 26,27 In the current study, 58 of 5796 E. coli isolates exhibited an increasing carbapenem-resistant rate from 2002 to 2017.…”

Section: Discussionmentioning

confidence: 56%

Molecular Mechanisms and Epidemiology of Carbapenem-Resistant Escherichia coli Isolated from Chinese Patients During 2002–2017

Tian¹,

Zheng²,

Sun³

et al. 2020

IDR

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Background: The emergence and spread of carbapenem-resistant Escherichia coli (E. coli) pose a serious threat to human health worldwide. This study aimed to investigate the molecular mechanisms underlying carbapenem resistance and their prevalence among E. coli in China. Methods: A collection of 5796 E. coli clinical isolates were collected from the First Affiliated Hospital of Wenzhou Medical University from 2002 to 2017. Sensitivity to antibiotics was determined using the agar dilution method. The detection of carbapenemases production and the prevalence of resistance-associated genes were investigated through modified carbapenem inactivation method (mCIM), PCR and sequencing. The mutations in outer membrane porins genes (ompC and ompF) were also analyzed by PCR and sequencing assays. The effect of efflux pump mechanism on carbapenem resistance was also tested. E. coli were typed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Results: A total of 58 strains (1.0%) of carbapenem-resistant E. coli were identified. The strains carrying bla KPC-2 and bla NDM accounted for 22.4% (13/58) and 51.7% (30/58), respectively. Among bla NDM -positive strains, 27 bla NDM genes were assigned to bla NDM-5 , while the remaining three strains were bla NDM-1 , whereas bla VIM , bla IMP , bla OXA-48 , and bla SHV were not found. The CTX-M-type β-lactamase genes accounted for 96.6% (56/58). In addition, bla TEM-1 genes were identified in 58.6% of tested strains. In carbapenem-resistant isolates, mutations in OmpC (the majority of mutated sites were D192G and Q104_F141del, accounting for 54.5%) and OmpF (large deletions S75_V127del, W83_D135del and Q88_D135del) were detected. Of note, the antibiotic resistance was not associated with overexpression of efflux pump. Moreover, MLST categorized the 58 carbapenem-resistant isolates into 19 different sequence types. PFGE analysis revealed that homology among the carbapenem-resistant isolates was low and sporadic. Conclusion: The bla NDM was the principal resistance mechanism of carbapenem-resistant E. coli in the hospital. bla NDM-5 is becoming a new threat to public health and the alteration of outer membrane porins might help further increase the MIC of carbapenem.

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Section: Discussionmentioning

confidence: 56%

Molecular Mechanisms and Epidemiology of Carbapenem-Resistant Escherichia coli Isolated from Chinese Patients During 2002–2017

Tian¹,

Zheng²,

Sun³

et al. 2020

IDR

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“…The pathogen that strains were most commonly resistant to varied from macrolides to cephalosporin among countries, and the pathogens associated with the highest level of sensitivity were carbapenem and tetracycline. Studies in Sudan have shown that P. aeruginosa strains are most frequently resistant toward penicillins [ 21 ], followed by cephalosporines [ 22 , 44 ], quinolones, nitrofuran, and tetracyclines [ 21 ], and aminoglycosides [ 44 ]. Resistance was least commonly found for carbapenem, aminoglycosides, and quinolones [ 22 ].…”

Section: Discussionmentioning

confidence: 99%

Distribution of Extended-Spectrum β-Lactamase (ESBL)-Encoding Genes among Multidrug-Resistant Gram-Negative Pathogens Collected from Three Different Countries

et al. 2021

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The incidence of Extended-spectrum β-lactamase (ESBL)-encoding genes (blaCTX-M and blaTEM) among Gram-negative multidrug-resistant pathogens collected from three different countries was investigated. Two hundred and ninety-two clinical isolates were collected from Egypt (n = 90), Saudi Arabia (n = 162), and Sudan (n = 40). Based on the antimicrobial sensitivity against 20 antimicrobial agents from 11 antibiotic classes, the most resistant strains were selected and identified using the Vitek2 system and 16S rRNA gene sequence analysis. A total of 85.6% of the isolates were found to be resistant to more than three antibiotic classes. The ratios of the multidrug-resistant strains for Egypt, Saudi Arabia, and Sudan were 74.4%, 90.1%, and 97.5%, respectively. Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa showed inconstant resistance levels to the different classes of antibiotics. Escherichia coli and Klebsiella pneumoniae had the highest levels of resistance against macrolides followed by penicillins and cephalosporin, while Pseudomonas aeruginosa was most resistant to penicillins followed by classes that varied among different countries. The isolates were positive for the presence of the blaCTX-M and blaTEM genes. The blaCTX-M gene was the predominant gene in all isolates (100%), while blaTEM was detected in 66.7% of the selected isolates. This work highlights the detection of multidrug-resistant bacteria and resistant genes among different countries. We suggest that the medical authorities urgently implement antimicrobial surveillance plans and infection control policies for early detection and effective prevention of the rapid spread of these pathogens.

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“…that were found in the investigated samples are mainly bacteria living in the natural environment and are an opportunistic pathogen. It often causes chronic lung infections associated with respiratory failure, clinic deterioration or burn patients 48,49. However, it should be pointed out that they are a source of endotoxins too.…”

Section: Discussionmentioning

confidence: 99%

Potential effects of microbial air quality on the number of new cases of diabetes type 1 in children in two regions of Poland: a pilot study

Michalska

Wąż

Zorena

et al. 2019

IDR

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Aim: The aim of the study was to investigate the relationship between the concentration of psychrophilic bacteria, mesophilic bacteria and mold fungi in bioaerosols, and the number of new cases of type 1 diabetes mellitus (T1DM) in children. Methods: Air samples from the Lubelskie and Pomeranian voivodeships in Poland were collected from January 2015 to December 2016 in winter, spring, summer and autumn. Thirty-three samples were collected in the Pomeranian and 27 in the Lubelskie voivodeship. The air samples were collected on the first day of each month at 1:00 pm for 10 mins at a height of 1.5 m above the ground. The number of mesophilic bacteria was detected after 24–48 hrs incubation at 37°C on tryptone soya agar (TSA; Merck, Darmstadt, Germany). The number of psychrophilic bacteria was detected after 72 hrs incubation at 22°C on TSA. The number of fungi was detected by a 5-day long incubation at 28°C on chloramphenicol yeast glucose agar. Results: In the Lubelskie voivodeship, the mean concentration of psychrophilic bacteria was significantly higher than in the Pomeranian voivodeship (2739 vs 608 CFU/m 3 , respectively), the mean concentration of mesophilic bacteria was significantly higher (2493 vs 778/m 3 , respectively) and the concentration of fungi was significantly higher (3840 vs 688 CFU/m 3 , respectively). We also showed a statistically significant relationship between the number of children with recently diagnosed T1DM and the mean concentration of psychrophilic and mesophilic bacteria in the Pomeranian and Lubelskie voivodeships ( P <0.001). Moreover, we found a significant relationship between the number of new cases of T1DM in children and the mean concentration of fungi in bioaerosols in the Lubelskie voivodeship ( P <0.001), but not in the Pomeranian voivodeship ( P =NS). Conclusion: The results of our research showed that there is a higher concentration of microbial particles in the Lublin voivodeship. Therefore, we recommend changes in climate for children (trips to the sea, mountains, etc) as often as possible.

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<p>Investigation of the prevalence of genes conferring resistance to carbapenems in <em>Pseudomonas aeruginosa</em> isolates from burn patients</p>

Cited by 18 publications

References 22 publications

<p>Molecular Mechanisms and Epidemiology of Carbapenem-Resistant <em>Escherichia coli</em> Isolated from Chinese Patients During 2002–2017</p>

<p>Molecular Mechanisms and Epidemiology of Carbapenem-Resistant <em>Escherichia coli</em> Isolated from Chinese Patients During 2002–2017</p>

Distribution of Extended-Spectrum β-Lactamase (ESBL)-Encoding Genes among Multidrug-Resistant Gram-Negative Pathogens Collected from Three Different Countries

<p>Potential effects of microbial air quality on the number of new cases of diabetes type 1 in children in two regions of Poland: a pilot study</p>

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