<p>Hsa_circ_0001806 Acts as a ceRNA to Facilitate the Stemness of Colorectal Cancer Cells by Increasing COL1A1</p>

Abstract

Background: The aberrant expression of circular RNAs (circRNAs) has been identified as a novel trait of cancers. However, the role of circRNAs in colorectal cancer (CRC) remains to be elucidated. Methods: Informatic analysis was performed to identify circRNAs in CRC tissues and adjacent tissues. Gain-and loss-of-function experiments were constructed to analyze hsa_-circ_001806 roles in CRC cell stemness by sphere-formation, ALDH activity, stemness marker expression and tumor-initiating ability assays. CCK8 cell viability was carried out to evaluate hsa_circ_0001806 roles in CRC cell viability. Luciferase reporter and pull-down assays were used to reveal the underlying mechanisms. Results: Hsa_circ_0001806 was significantly upregulated in CRC tissues and correlated with TNM stage, depth of invasion, lymphatic metastasis and distant metastasis. Hsa_circ_0001806 promoted the stemness of CRC cells, as evident by increasing sphereformation ability, ALDH1 activity and stemness marker expression while had no effect on cell viability. Mechanistically, the same miR-193-5p-binding sites are shared between hsa_-circ_0001806 and COL1A1. Hsa_circ_0001806 upregulates COL1A1 expression in a miR-193-5p-dependent manner, which is essential for hsa_circ_0001806-mediated regulation on CRC cell stemness. Conclusion: CircRNA hsa_circ_0001806 may act as a promising therapeutic target by facilitating the stemness of CRC cells via activating the hsa_circ_0001806/miR-193a-5p/ COL1A1 axis.

Identification of circRNA circ-CSPP1 as a potent driver of colorectal cancer by directly targeting the miR-431/LASP1 axis

“…Several recent studies have reported the oncogenic role of circ-CSPP1 in ovarian cancer, cervical cancer, glioma, and hepatocellular carcinoma [ 21 , 22 , 23 , 24 ]. Here, our work showed a remarkable overexpression of circ-CSPP1 in CRC, in agreement with the recent work [ 10 ]. Furthermore, we presented a significant clinical association of circ-CSPP1 with the poor prognosis of CRC patients.…”

“…Despite these studies, the precise actions of circRNAs in CRC development have remained unclear. circ-CSPP1 was recently identified as a circRNA whose overexpression in CRC contributed to the stemness of CRC cells [ 10 ]. Our current study expanded on this by identifying circ-CSPP1 as a crucial regulator in CRC cell malignant behaviors.…”

“…Such analyses are hampered at present by the lack of the direct evidence in vivo of the novel mechanism, the circ-CSPP1/miR-431/LASP1 axis, in regulating CRC progression. Previous work reported the promoting effect of circ-CSPP1 on the stemness of CRC cells through the miR-193a-5p/COL1A1 axis [ 10 ]. Recent studies also identified the oncogenic role of circ-CSPP1 in human carcinogenesis by regulating the miR-361-5p/ITGB1 or miR-577/CCNE2 axis [ 22 , 24 ].…”

“…Bian et al illuminated that hsa_circ_103809 controlled the carcinogenesis of CRC through targeting miR-532-3p [ 9 ]. As for circRNA centrosome and spindle pole-associated protein 1 (circ-CSPP1, hsa_circ_0001806), an upregulated circRNA in CRC, it was reported to be implicated in the stemness of CRC cells through the modulation of miR-193-5p [ 10 ]. However, no insight has been gained into the precise parts of circ-CSPP1 in CRC progression.…”

Oncogenic Functions and Clinical Significance of Circular RNAs in Colorectal Cancer

“…CircCSPP1 (hsa_circ_0001806, hsa_circ_001780) also is involved in the process of EMT and its high levels are associated with higher liver metastasis risk [ 153 ]. Wang et al, 2020 [ 154 ] explained the role of circCSPP1 in enhancing CRC cell migration and invasion by upregulation of COL1A1 (collagen, type I, alpha 1) expression from circRNA in a miR-193-5p-dependent way.…”