&lt;p&gt;Genotypic Distribution and a Potential Diagnostic Assay of Multidrug-Resistant Tuberculosis in Northern Thailand&lt;/p&gt;

Anukool, Usanee; Phunpae, Ponrut; Tharinjaroen, Chayada Sitthidet; Butr-Indr, Bordin; Saikaew, Sukanya; Netirat, Nathiprada; Intorasoot, Sorasak; Suthachai, V.; Tragoolpua, Khajornsak; Chaiprasert, Angkana

doi:10.2147/idr.s263082

Cited by 10 publications

(23 citation statements)

References 22 publications

(44 reference statements)

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“…In comparison, our study in Can Tho found no substitution in this codon. However, our study detected a substitution mutation at codon 532 that causes rifampicin resistance, while prior studies had not found it [22][23][24][25]27,[29][30][31][32][33][34][35] . Fitzgibbon, Margaret M., et al 2021 revealed mutations in Condon 532 26 ; however, the study's conclusion was due to the detection error of Xpert ® MTB/RIF Ultra.…”

Section: Country or Regioncontrasting

confidence: 58%

“…Table 8 compares the difference in mutant genotypes of rifampicin-resistant TB bacteria in our study to studies from other geographical regions. Codon 526 is one of the most prevalent mutations in the RRDR of the rpoB gene [25][26][27][28] . Mutations at codon 526 had been found in previous studies 23,25,27,[29][30] .…”

Section: Country or Regionmentioning

confidence: 99%

“…Codon 526 is one of the most prevalent mutations in the RRDR of the rpoB gene [25][26][27][28] . Mutations at codon 526 had been found in previous studies 23,25,27,[29][30] . In comparison, our study in Can Tho found no substitution in this codon.…”

Section: Country or Regionmentioning

confidence: 99%

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Prevalence of drug-resistant recurrent tuberculosis and new multidrug-resistant tuberculosis mutations detection in Can Tho, Vietnam

Duong¹,

Tan²,

Tran³

et al. 2022

Pharm Sci Asia

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Tuberculosis (TB) has been a threat to world health for decades. Multidrug-resistant TB (MDR-TB) is adding to the burden of disease and hindering the development of countries. This study aimed to identify the drug-resistant rate in patients with recurrent pulmonary TB and detect rifampicin resistance mutations in the rpoB gene in Can Tho, Vietnam. In this descriptive cross-sectional study, clinicians diagnosed the patients with recurrent pulmonary TB and were hospitalized. Smear-positive sputum specimens (n=246) were collected to determine the rate of drug resistance. To identify mutations in the rpoB gene which are likely to be associated with rifampicin resistance in MDR-TB, we sequenced 40 isolates of Mycobacterium tuberculosis collected between 2012 and 2014; mainly MDR-TB (n=40; 95.2%) and rifampicin-sensitive TB (n=2; 4.8%). The rate of drug-resistant pulmonary tuberculosis was 63.8%, and the rate of MDR-TB accounted for 23.2%. The mutations in rpoB were predominantly in codons 531 (27.5%) and 523 (17.5%), with rare occurrences of S522A (2.5%) and A532P (2.5%). Noticeably, there was a substitution mutation in codon 532, and a mutant strain of tuberculosis at seven codons in the rpoB gene had been detected. In conclusion, this study provided drug-resistant characteristics in patients with recurrent pulmonary TB and mutations of MDR-TB in Can Tho, Vietnam. The results show a mutation in codon 532 of the rpoB gene and a mutant strain of tuberculosis at all seven codons. These are remarkable and promising results for further studies and clinical applications.

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Section: Country or Regioncontrasting

confidence: 58%

Section: Country or Regionmentioning

confidence: 99%

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Prevalence of drug-resistant recurrent tuberculosis and new multidrug-resistant tuberculosis mutations detection in Can Tho, Vietnam

Duong¹,

Tan²,

Tran³

et al. 2022

Pharm Sci Asia

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“…First, the WHO-endorsed GeneXpert was widely used in this region, allowing for the rapid diagnosis of MDR-/RR-TB cases compared with the speed of conventional phenotypic methods. 30 Second, all identified MDR-TB patients were hospitalized until bacterial conversion was verified by smear microscopy. Significant reductions in the bacterial load of sputum samples can reduce the transmission of MDR-TB in the community.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Multidrug-Resistant Tuberculosis in Dalian, China: A Retrospective Study

Zhang

Lv³

et al. 2021

IDR

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Purpose Multidrug-resistant tuberculosis (MDR-TB) is the cause of serious health and economic burdens worldwide. The present study aimed to explore the initial and acquired drug-resistance rates among TB patients from 2012 to 2019 in Dalian, China. The effectiveness of MDR-TB prevention and control strategies were then evaluated. Patients and Methods Drug susceptibility testing (DST) was performed for 6429 diagnosed, culture-positive, Mycobacterium tuberculosis (MTB) strains, including 4661 new cases and 1768 previously treated cases. Descriptive statistics were employed to calculate the frequencies and percentages of TB strains, and the average annual growth rates (AAGRs) for each strain were calculated. The Chi-square test was applied to examine the significance of linear drug-resistance trends over time during the study period. Results Over the eight-year study period, the percentages of both initial (from 9.01% to 4.82%) and acquired (from 40.85% to 9.09%) MDR-TB cases decreased significantly, AAGRs of 8.55% and 19.32%, respectively. Among new and previously treated TB patients, significant downtrends were observed for the rates of both initial and acquired MDR-TB among young and middle-aged individuals ( P < 0.05). Additionally, among both new and previously treated TB patients, the percentages of individuals with drug resistance against isoniazid (INH), rifampicin (RFP), ofloxacin (OFX), and amikacin (AMK) decreased significantly ( P < 0.05) from 2012 to 2019 in Dalian, China. Conclusion The initial and acquired multidrug resistance rates exhibited significantly decreasing trends from 2012 to 2019, suggesting that MDR-TB prevalence has been controlled effectively in Dalian, China. The MDR-TB epidemic was reversed in the short term by establishing feasible strategies for detection, diagnosis, treatment, and infection control.

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“…Globally in 2019, an estimated 465,000 incident cases were reported as MDR and rifampicin resistant (RR) TB, while 3.3% and 13% of new cases and 18% and 17.4% of previously treated cases were diagnosed with MDR/RR TB and isoniazid (INH) resistant TB, respectively (World Health Organization 2020). Thailand is one of the top 14 countries with high TB, TB/HIV and MDR-TB and had 150 TB cases per 100,000 population in 2019 (World Health Organization 2020) (Anukool et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Novel mutations detected from drug resistant Mycobacterium tuberculosis isolated from North East of Thailand

Thwe

Namwat

Pinlaor

et al. 2021

World J Microbiol Biotechnol

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The emergence of drug-resistant tuberculosis is a major global public health threat. Thailand is one of the top 14 countries with high tuberculosis, tuberculosis co-infected with Human Immunodeficiency Virus (HIV) and multi-drug resistant tuberculosis rates. Immediate detection of drug-resistant tuberculosis is necessary to reduce mortality and morbidity by effectively providing treatment to ameliorate the formation of resistant strains. Limited data exist of mutation profiles in Northeastern Thailand. Here, 65 drug-resistant Mycobacterium tuberculosis isolates were used to detect mutations by polymerase chain reaction (PCR) and DNA sequencing. In the katG gene, mutations occurred in 47(79.7%) among 59 isoniazid resistant samples. For rpoB gene, 31 (96.9%) were observed as mutations in 32 rifampicin resistant isolates. Of 47 katG mutation samples, 45 (95.7%) had mutations in katG315 codon and 2 (4.3%) showed novel mutations at katG365 with amino acid substitution of CCG-CGG (Pro-Arg). Moreover, 18 (58.1%) mutations at rpoB531, 11 (35.5%) mutations at rpoB516, 10 (32.3%) mutations at rpoB526 and 1 (3.2%) mutation at rpoB533 were found between 31 rifampicin resistant samples. Common and novel mutations of the rpoB and katG genes in Northeastern Thailand were generated. DNA sequencing showed high accuracy, while conventional polymerase chain reaction could be applied as an initial marker for screening rifampicin and isoniazid drug-resistant Mycobacterium tuberculosis in Northeastern Thailand. Mutations reported here should be considered when developing new molecular diagnostic methods for implementation in Northeastern Thailand.

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<p>Genotypic Distribution and a Potential Diagnostic Assay of Multidrug-Resistant Tuberculosis in Northern Thailand</p>

Cited by 10 publications

References 22 publications

Prevalence of drug-resistant recurrent tuberculosis and new multidrug-resistant tuberculosis mutations detection in Can Tho, Vietnam

Prevalence of drug-resistant recurrent tuberculosis and new multidrug-resistant tuberculosis mutations detection in Can Tho, Vietnam

Prevalence of Multidrug-Resistant Tuberculosis in Dalian, China: A Retrospective Study

Novel mutations detected from drug resistant Mycobacterium tuberculosis isolated from North East of Thailand

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