&lt;p&gt;Evaluation of Antimalarial Activity of Hydromethanolic Crude Extract and Solvent Fractions of the Leaves of &lt;em&gt;Nuxia congesta&lt;/em&gt; R. Br. Ex Fresen (&lt;em&gt;Buddlejaceae&lt;/em&gt;) in &lt;em&gt;Plasmodium berghei&lt;/em&gt; Infected Mice&lt;/p&gt;

Fenta, Melshew; Kahaliw, Wubayehu

doi:10.2147/jep.s230636

Cited by 17 publications

(21 citation statements)

References 39 publications

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“…It could also be associated with the amount of hypothermic components present in the crude extracts and chloroform fractions of the test plant 46,47. Furthermore it could be attributed to the inability of the extracts and chloroform fractions to completely clear the parasites from the body of the mice other than reducing to different levels. 29,37 This is comparably similar with the findings of other plants such as Leonoti socymifolia, 47 Calpurnia aurea 48 and D. angustifolia extracts, 40 where these plants were unable to prevent the drop of weight and temperature, although they showed significant parasitemia suppression. Indeed, it has been reported that the animals those infected with plasmodium showed a decline in food consumption and body weight during the course of the experiment.…”

Section: Discussionsupporting

confidence: 79%

“…This is comparable to the activities of Nuxia congesta and Melia azedarach in previous studies. 40,41 Figure 1 Comparison of packed cell volume of P. Berghei infected Swiss albino mice treated with hydroalcoholic crude extract and solvent fraction of Zehneria scabra roots in the 4-day suppressive test. Notes: a, compared to CON; b, to CQ25; c, to 200 mg/kg: 1 p<0.05, 2 p<0.01, 3 p<0.001; CON, negative control, received distilled water 10 mL/kg; ZS = crude extract of Zehneria scabra, and CQ = chloroquine; numbers (25,200,400,600) refer to dosein mg/kg, D0 = day0, D4 = day 4.…”

Section: Discussionmentioning

confidence: 99%

“…This is comparable to the activities of Nuxia congesta and Melia azedarach in previous studies. 40 , 41 …”

Section: Discussionmentioning

confidence: 99%

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Evaluation of Antiplasmodial Activity of Hydroalcoholic Crude Extract and Solvent Fractions of Zehneria scabra Roots Against Plasmodium berghei in Swiss Albino Mice

Nureye¹,

Tekalign²,

Fisseha³

et al. 2021

IDR

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Background: Since drug resistance makes controlling malaria parasites a major challenge, these pioneering researchers explore and discover new novel drugs from a variety of sources. As a result, this study aimed to assess the anti-plasmodial activity of hydroalcoholic crude extract and solvent fractions of Zehneria scabra roots in mice infected with Plasmodium berghei. Methods: The antimalarial activity and safety profile of Zehneria scabra extracts were tested in a mouse model using four-day suppressive, prophylactic, and rane's tests against chloroquine-sensitive Plasmodium berghei. Mice were divided into five groups at random: group I received distilled water (10 mL/kg), group II, III, and IV received 200, 400, and 600 mg/kg of the extract, respectively, and group V received chloroquine (25 mg/kg). The antimalarial activity of the extract was determined using parasitemia levels, survival time, rectal temperature, and weight variation. Results: At all dose levels, the crude extract and solvent fractions of Zehneria scabra showed significant (p<0.05 to p<0.001) chemosuppression, with the crude extract and butanol fraction showing the highest chemosuppression (73.09% and 74.09%, respectively). Apart from suppressing parasitemia, the extract also increased survival time and secured packed cell volume reduction substantially (p<0.05 to p<0.001), while the crude extract had no significant impact on body weight or rectal temperature reduction in four-day suppressive and prophylactic models. Conclusion:The result designated that Zehneria scabra is endowed with significant antimalarial activity. These results thus support the traditional use of Zehneria scabra, for the treatment of malaria.

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Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 99%

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Evaluation of Antiplasmodial Activity of Hydroalcoholic Crude Extract and Solvent Fractions of Zehneria scabra Roots Against Plasmodium berghei in Swiss Albino Mice

Nureye¹,

Tekalign²,

Fisseha³

et al. 2021

IDR

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“…In addition, some flavonoids have also been reported to inhibit the growth of malaria parasites by inhibiting the influx of l -glutamine and myoinositol into infected erythrocytes [ 34 ]. Therefore, antimalarial effect of aqueous extract of S. exigua root against P. berghei ANKA might be attributed to a single or a combination of its secondary metabolites, leading to alteration of protein structure and impairment of its function and inhibition of blood stage propagation of parasites [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Antioxidant and antimalarial properties of Sophora exigua Craib. root extract in Plasmodium berghei-infected mice

et al. 2021

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Background Sophora exigua Craib. is commonly used in Thailand to reduce fever and increase postpartum breast milk production in women who have hypogalactia. However, there has been no report on the antioxidant and antimalarial properties of this plant. This study aimed to investigate the antioxidant and antimalarial activities of S. exigua root extract and to evaluate its acute toxicity in mice to confirm its safety. Methods The in vitro antioxidant activities were determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH), superoxide radical, and hydroxyl radical scavenging assays. The in vivo antioxidant activities were determined by detecting the malondialdehyde (MDA) content and superoxide dismutase (SOD) activity in the livers of malaria-infected mice. The in vivo antimalarial activity was determined by Peters’ 4-day suppressive test in mice infected with Plasmodium berghei ANKA and orally administered S. exigua root aqueous and ethanolic extracts at different doses (200, 400, and 600 mg/kg body weight). In addition, the acute oral toxicity of the plant extracts was assessed in mice at a dose of 2000 mg/kg body weight. Results The ethanolic extract of S. exigua root exhibited inhibition of DPPH radicals, superoxide anions, and hydroxyl radicals, with half maximal inhibitory concentration (IC50) values of 24.63 ± 1.78, 129.78 ± 0.65, and 30.58 ± 1.19 μg/ml, respectively. Similarly, research on the in vivo antioxidant activity indicated that the ethanolic extract of S. exigua root exerted a stronger effect than the aqueous extract. The aqueous extract at doses of 200, 400, and 600 mg/kg had stronger antimalarial activity than the ethanolic extract. The aqueous extract at 600 mg/kg exhibited 60.46% suppression of parasitemia. Increased levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) and blood urea nitrogen (BUN) were detected in the mice treated with 2000 mg/kg ethanolic extract, which was related to the results of histopathological analysis of liver tissue, showing ballooning degeneration of hepatocytes, diffuse hepatic hemorrhage, and infiltration of inflammatory cells. Conclusions This study demonstrated that the ethanolic S. exigua root extract possessed antioxidant properties, and the aqueous extract also had antimalarial activity. Therefore, this plant is an alternative source of new antioxidant and antimalarial agents.

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“…Malaria remains one of the life-threatening infectious diseases in tropical and subtropical regions of the world [ 1 ]. Of the five species of Plasmodium parasites that cause human malaria, Plasmodium falciparum is the most pathogenic species with the greatest likelihood of drug resistance [ 2 , 3 ].…”

Section: Introductionmentioning

confidence: 99%

Antimalarial properties and molecular docking analysis of compounds from Dioscorea bulbifera L. as new antimalarial agent candidates

Chaniad

Mungthin

Payaka

et al. 2021

BMC Complement Med Ther

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Background At present, the emergence and spread of antimalarial drug resistance has become a significant problem worldwide. There has been a challenge in searching for natural products for the development of novel antimalarial drugs. Therefore, this study aims to evaluate compounds from Dioscorea bulbifera responsible for antimalarial properties and investigate potential interactions of the compounds with Plasmodium falciparum lactate dehydrogenase (PfLDH), an essential glycolytic enzyme in the parasite’s life cycle. Methods An in vitro study of antimalarial activity against chloroquine (CQ)-resistant Plasmodium falciparum (K1 strain) and CQ-sensitive P. falciparum (3D7 strain) was performed using the 3H-hypoxanthine uptake inhibition method. The cytotoxic effects of the pure compounds were tested against Vero cells using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The interactions of the compounds with the PfLDH active site were additionally investigated using a molecular docking method. Results Quercetin (6) exhibited the highest antimalarial activity against the P. falciparum K1 and 3D7 strains, with IC50 values of 28.47 and 50.99 μM, respectively. 2,4,3′,5′-Tetrahydroxybibenzyl (9), 3,5-dimethoxyquercetin (4) and quercetin-3-O-β-D-galactopyranoside (14) also possessed antimalarial effects against these two strains of P. falciparum. Most pure compounds were nontoxic against Vero cells at a concentration of 80 μg/ml, except for compound 9, which had a cytotoxic effect with a CC50 value of 16.71 μM. The molecular docking results indicated that 9 exhibited the best binding affinity to the PfLDH enzyme in terms of low binding energy (− 8.91 kcal/mol) and formed strong hydrogen bond interactions with GLY29, GLY32, THR97, GLY99, PHE100, THR101 and ASN140, amino acids as active sites. In addition, 6 also possessed remarkable binding affinity (− 8.53 kcal/mol) to PfLDH by interacting with GLY29, ILE31, ASP53, ILE54, THR97 and THR101. Conclusion Quercetin is a major active compound responsible for the antimalarial activity of D. bulbifera and is an inhibitor of PfLDH. These findings provide more evidence to support the traditional use of D. bulbifera for malaria treatment. Structural models of its interactions at the PfLDH active site are plausibly useful for the future design of antimalarial agents.

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<p>Evaluation of Antimalarial Activity of Hydromethanolic Crude Extract and Solvent Fractions of the Leaves of <em>Nuxia congesta</em> R. Br. Ex Fresen (<em>Buddlejaceae</em>) in <em>Plasmodium berghei</em> Infected Mice</p>

Cited by 17 publications

References 39 publications

Evaluation of Antiplasmodial Activity of Hydroalcoholic Crude Extract and Solvent Fractions of Zehneria scabra Roots Against Plasmodium berghei in Swiss Albino Mice

Evaluation of Antiplasmodial Activity of Hydroalcoholic Crude Extract and Solvent Fractions of Zehneria scabra Roots Against Plasmodium berghei in Swiss Albino Mice

Antioxidant and antimalarial properties of Sophora exigua Craib. root extract in Plasmodium berghei-infected mice

Antimalarial properties and molecular docking analysis of compounds from Dioscorea bulbifera L. as new antimalarial agent candidates

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