&lt;p&gt;Emerging roles of circular RNAs in colorectal cancer&lt;/p&gt;

Hao, Shuhong; Liu, Cong; Qu, Rongfeng; Liu, Rui; Zhang, Guizhen; Li, Yarong

doi:10.2147/ott.s208235

Cited by 39 publications

(33 citation statements)

References 121 publications

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“…Due to their special circular structure, circRNAs have high stability and great potential as new tumour markers [21]. Recent studies have found that circRNAs are involved in the development and progression of various cancers [22][23][24], such as gastric cancer, breast cancer, and colorectal cancer. Current evidence suggests that circRNAs can target miRNAs, often referred to as "miRNA sponges, " to reduce the levels of miRNAs and release their targeted inhibition of mRNAs, thereby regulating the expression of protein-coding genes [25][26][27].…”

Section: Discussionmentioning

confidence: 99%

Construction of a circRNA-miRNA-mRNA network based on competitive endogenous RNA reveals the function of circRNAs in osteosarcoma

Qiu

Chen

et al. 2020

Cancer Cell Int

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Background: Osteosarcoma (OS) is a common primary malignant bone tumour. Growing evidence suggests that circular RNAs (circRNAs) are closely related to the development of tumours. However, the function of circRNAs in OS remains unknown. Here, we aimed to determine the regulatory mechanisms of circRNAs in OS. Methods:The expression profiles of OS circRNA (GSE96964), microRNA (GSE65071) and mRNA (GSE33382) were downloaded from the Gene Expression Omnibus (GEO) database to identify differentially expressed circRNAs, miRNAs and mRNAs in OS. A ceRNA network was constructed based on circRNA-miRNA pairs and miRNA-mRNA pairs. MRNAs with significant prognostic differences were identified by the TARGET database in the network. Functional and pathway enrichment analyses were performed, and interactions between proteins were predicted using Cytoscape. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed to elucidate the possible functions of these differentially expressed circRNAs. Results:A total of 15 downregulated circRNAs, 136 upregulated miRNAs and 52 downregulated mRNAs were identified in OS. Finally, a circRNA-miRNA-mRNA network was constructed in OS based on 14 circRNAs, 24 miRNAs, and 52 mRNAs. GO and KEGG pathway analyses suggested that the mRNAs in the network may be involved in the pathogenesis and progression of OS. Four mRNAs identified by the TARGET database were significantly associated with OS survival prognosis. A circRNA-miRNA-mRNA subnetwork was constructed based on these four mRNAs. Conclusion:Our results provide a deeper understanding of the regulatory mechanisms by which circRNAs compete for endogenous RNAs in OS.

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Section: Discussionmentioning

confidence: 99%

Construction of a circRNA-miRNA-mRNA network based on competitive endogenous RNA reveals the function of circRNAs in osteosarcoma

Qiu

Chen

et al. 2020

Cancer Cell Int

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“…Interestingly, using primary cultures of human smooth muscle cells and macrophages as a model of atherogenesis, the authors demonstrated that impaired ribosome biogenesis and nucleolar stress-induction due to high levels of circANRIL, confers a protective response against atherosclerosis [117]. This mechanism of regulation of ribosome biogenesis through circular lncRNA provides exciting opportunities for investigating ribosome biogenesis in a different yet complementary context to its implication in the regulation of translation in CRC [118].…”

Section: Pes1 and Interacting Partners In Crcmentioning

confidence: 99%

Ribosome Biogenesis Alterations in Colorectal Cancer

Slimane

Marcel

Fenouil

et al. 2020

Cells

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Many studies have focused on understanding the regulation and functions of aberrant protein synthesis in colorectal cancer (CRC), leaving the ribosome, its main effector, relatively underappreciated in CRC. The production of functional ribosomes is initiated in the nucleolus, requires coordinated ribosomal RNA (rRNA) processing and ribosomal protein (RP) assembly, and is frequently hyperactivated to support the needs in protein synthesis essential to withstand unremitting cancer cell growth. This elevated ribosome production in cancer cells includes a strong alteration of ribosome biogenesis homeostasis that represents one of the hallmarks of cancer cells. None of the ribosome production steps escape this cancer-specific dysregulation. This review summarizes the early and late steps of ribosome biogenesis dysregulations described in CRC cell lines, intestinal organoids, CRC stem cells and mouse models, and their possible clinical implications. We highlight how this cancer-related ribosome biogenesis, both at quantitative and qualitative levels, can lead to the synthesis of ribosomes favoring the translation of mRNAs encoding hyperproliferative and survival factors. We also discuss whether cancer-related ribosome biogenesis is a mere consequence of cancer progression or is a causal factor in CRC, and how altered ribosome biogenesis pathways can represent effective targets to kill CRC cells. The association between exacerbated CRC cell growth and alteration of specific steps of ribosome biogenesis is highlighted as a key driver of tumorigenesis, providing promising perspectives for the implementation of predictive biomarkers and the development of new therapeutic drugs.

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“…Despite many advances achieved in diagnosis and therapy, the prognosis of CRC patients remains unsatisfactory due to the advanced stage at first diagnosis and the high frequency of metastasis and recurrence (2). Statistics from well-known sources have reported that the 5-year survival rates for CRC patients with early-stage disease was 90%, and the survival rate was only 13.1% for those diagnosed with late-stage CRC (3,4). Therefore, to raise the efficiency of tumor diagnosis and improve the prognosis of CRC patients, new sensitive and cost-effective biomarkers are urgently required.…”

Section: Introductionmentioning

confidence: 99%

RNA-Seq Profiling of Serum Exosomal Circular RNAs Reveals Circ-PNN as a Potential Biomarker for Human Colorectal Cancer

Xie

et al. 2020

Front. Oncol.

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Circular RNAs (circRNAs) are emerging as cardinal areas of focus in the non-coding RNA field. Growing evidences have revealed exosomal circRNAs as potential biomarkers for detection of various cancers. However, the clinical importance of most serum exosomal circRNAs in colorectal cancer (CRC) have rarely been investigated. In this study, we examined the possible clinical application of serum exosomal circRNAs in the diagnosis of CRC. Firstly, we conducted RNA sequencing (RNA-seq) analysis using fifty CRC and fifty healthy control serum samples to identify CRC-related circRNAs. The sequencing data showed 122 differentially expressed circRNAs including 100 up-regulated and 22 down-regulated circRNA transcripts in CRC patients. Then, eight most dysregulated circRNAs were selected for validation by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assay. Validation analysis revealed that the serum exosomal circ-PNN (hsa_circ_0101802) levels were significantly up-regulated in CRC cases compared with those in the healthy control groups. Receiver operating characteristic curve (ROC) analysis suggested that circ-PNN had significant value in CRC diagnosis with areas under the ROC curve (AUC) of 0.855 and 0.826 in the training and validation sets, respectively. We also found that the AUC of serum exosomal circ-PNN for early-stage CRC was 0.854. Finally, a network map based on circ-PNN was constructed to determine its potential miRNA-mRNAs binding. We also demonstrated that the expression of hsa-miR-6833-3P, hsa-let-7i-3p and hsa-miR-1301-3P were negatively correlated with circ-PNN in CRC patients. Collectively, our findings indicated that serum exosomal circ-PNN might be a potential non-invasive biomarker for the detection of CRC and may play a crucial role in the pathogenesis of CRC.

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<p>Emerging roles of circular RNAs in colorectal cancer</p>

Cited by 39 publications

References 121 publications

Construction of a circRNA-miRNA-mRNA network based on competitive endogenous RNA reveals the function of circRNAs in osteosarcoma

Construction of a circRNA-miRNA-mRNA network based on competitive endogenous RNA reveals the function of circRNAs in osteosarcoma

Ribosome Biogenesis Alterations in Colorectal Cancer

RNA-Seq Profiling of Serum Exosomal Circular RNAs Reveals Circ-PNN as a Potential Biomarker for Human Colorectal Cancer

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