&lt;p&gt;Emergence of two &lt;em&gt;Escherichia coli&lt;/em&gt; strains co-harboring &lt;em&gt;mcr-1&lt;/em&gt; and bla&lt;sub&gt;NDM&lt;/sub&gt; in fresh vegetables from China&lt;/p&gt;

Liu, Bao Tao; Song, Feng Jing

doi:10.2147/idr.s211746

Cited by 28 publications

(20 citation statements)

References 41 publications

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“…In this study, pAH25-1 was closely aligned with IncI2 plasmid pBA76-MCR-1 from E. coli in humans in the Arabian Peninsula (22). pAH62-1 was closely aligned with IncI2 plasmid pZJ3920-3 from E. coli in humans in Hangzhou, China, and pAH65-1 was closely aligned with IncI2 plasmid pP111 from Salmonella enterica serovar Typhimurium in pigs in Taiwan (23). This phenomenon emphasized that IncI2 plasmid posed a risk for the exchange of genetic material between different niches.…”

Section: Discussionmentioning

confidence: 56%

Complete Genetic Analysis of Plasmids Carrying mcr-1 and Other Resistance Genes in Avian Pathogenic Escherichia coli Isolates from Diseased Chickens in Anhui Province in China

Yin

Cheng

Yang

et al. 2021

mSphere

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Antimicrobial resistance associated with colistin has emerged as a significant concern worldwide, threatening the use of one of the most important antimicrobials for treating human disease. This study aimed to investigate the prevalence of colistin-resistant avian-pathogenic Escherichia coli (APEC) and shed light on the possibility of transmission of mcr-1 (mobilized colistin resistance)-positive APEC. A total of 72 APEC isolates from Anhui Province in China were collected between March 2017 and December 2018 and screened for the mcr-1 gene. Antimicrobial susceptibility testing was performed using the broth dilution method. Pulsed-field gel electrophoresis, Southern blot analysis, and conjugation assay were performed to determine the location and conjugative ability of the mcr-1 gene. Whole-genome sequencing and analysis were performed using Illumina MiSeq and Nanopore MinION platforms. Three APEC isolates (AH25, AH62, and AH65) were found to be positive for the mcr-1 gene and showed multidrug resistance. The mcr-1 genes were located on IncI2 plasmids, and conjugation assays revealed that these plasmids were transferrable. Notably, strains AH62 and AH65, both belonging to ST1788, were collected from different places but carried the same drug resistance genes and shared highly similar plasmids. This study highlights the potential for a possible epidemic of mcr-1-positive APEC and the urgent need for continuous active monitoring. IMPORTANCE In this study, three plasmids carrying mcr-1 were isolated and characterized from APEC isolates from Anhui Province in China. The mcr-1 genes were located on IncI2 plasmids, and these plasmids were transferrable. These three IncI2 plasmids had high homology with the plasmids harbored by pathogenic bacteria isolated from other species. This finding showed that IncI2 plasmids poses a risk for the exchange of genetic material between different niches. Although colistin has been banned for use in food-producing animals in China, the coexistence of the broad-spectrum β-lactamase and mcr-1 genes on a plasmid can also lead to the stable existence of mcr-1 genes. The findings illustrated the need to improve the monitoring of drug resistance in poultry systems so as to curb the transmission or persistence of multidrug-resistant bacteria.

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Section: Discussionmentioning

confidence: 56%

Complete Genetic Analysis of Plasmids Carrying mcr-1 and Other Resistance Genes in Avian Pathogenic Escherichia coli Isolates from Diseased Chickens in Anhui Province in China

Yin

Cheng

Yang

et al. 2021

mSphere

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“… 23 Moreover, an antimicrobial resistance gene conferring resistance to fosfomycin, fosA3 , is located upstream of NDM-9 region. To date, the emergence of bla NDM-9 has been reported in E. coli , 24 , 25 Klebsiella pneumoniae , 10 Klebsiella variicola , 11 and Cronobacter sakazakii . 26 Our discovery of this plasmid supplemented previous studies and further highlighted the dissemination of bla NDM-9 gene-carrying plasmids in human gut colonized isolates.…”

Section: Resultsmentioning

confidence: 99%

Co-Occurrence of NDM-9 and MCR-1 in a Human Gut Colonized Escherichia coli ST1011

Liang¹,

Rao²,

Wang³

et al. 2021

IDR

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Background The emergence of the plasmid-borne colistin-resistant gene ( mcr-1 ) poses a great threat to human health. What is worse, the recent observations of the coexistence of mcr-1 with carbapenemase encoding genes in some bacteria caused even more concern. Yet, there is a lack of observations of such strains in the human gut. Methods The isolation of E. coli L889 was performed on selective medium plates. Antibiotic susceptibilities were determined by an agar dilution and a broth microdilution method. Multi-locus sequence typing (MLST) and acquired resistance genes were also characterized. Transferability of bla NDM-9 / mcr-1 -carrying plasmids was determined by conjugation, replicon typing and S1-Pulsed-field gel electrophoresis (S1-PFGE), and Southern blotting. The sequences of these plasmids were analyzed by using whole-genome sequencing with Illumina Novaseq and Nanopore platforms. Results E. coli L889 was identified as ST1101 concomitantly carrying bla NDM-9 and mcr-1 from a stool sample. Antimicrobial susceptibility tests showed that it was resistant to various antimicrobial agents and only susceptible to tigecycline. Notably, bla NDM-9 was located on a ~114-kb untypable plasmid, while mcr-1 was located on a ~63-kb IncI2 plasmid. Conclusion Our research, to our knowledge, first reported an ST1101 E. coli strain with an untypeable bla NDM-9 -harbouring plasmid and an IncI2 mcr-1 -carrying plasmid. The colonized E. coli strains potentially contribute to the dissemination and transfer of bla NDM-9 and mcr-1 to clinical isolates, which is a considerable threat to public health and should be closely monitored.

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“…We detected ve strains of Acinetobacter and one strains of K. pneumoniae co-producing mcr-1 and NDM-5 in the present study. It has been reported that both bla NDM and mcr genes are commonly present in E. coli isolated from different sources [26][27][28][29], but the co-existence of the bla NDM and mcr genes in K. pneumoniae has rarely been reported. In this study, the plasmid harboring the mcr gene in K. pneumoniae was similar to other IncHI2-type plasmids present in E. coli, and upstream of the mcr gene was the common ISApl1 transposon.…”

Section: Discussionmentioning

confidence: 99%

Characterization of carbapenem non-susceptible Gram-negative Bacilli isolated from the feces of 10,000 inpatients in Southern China

Duan

Yuan

et al. 2021

Preprint

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Background: Carbapenem non-susceptible Gram-negative bacilli (CNS-GNB) were dominant pathogen causing clinical infections. The human intestine was important reservoir of gram-negative bacilli (GNB), but there were few studies to analysis the prevalence of fecal colonization with them.Methods: Fecal samples were collected from hosiptal screening test for GNB was conducted by using home-made MacConkey agar. Antimicrobial susceptibility was determined by the automatic microbiology analyzer and drug-resistant genes were characterized by polymerase chain reaction assays and DNA sequencing. The whole genome sequencing were used to analysis the characteristic of genetic structure of the isolates.Results: A total of 680 CNS-GNB were collected. Acinetobacter spp. were the dominant genus (33.8%, 230/680) of the 22 genera. Carbapenemase genes were identified in 307 isolates (45.1%, 307/680), including 206 (30.3%, 206/680) blaNDM; 51 (7.5%, 51/680) blaVIM-2, 48 (7.1%, 48/680) blaIMP, and seven (1.0%, 7/680) blaKPC-2. The blaNDM genes were first detected in three isolates, Providencia vermicola, Achromobacter spp., and Cupriavidus gilardii. Co-existence of blaVIM and blaIMP genes was detected in five isolates; Achromobacter co-producing VIM and IMP has not been previously reported. The mcr-1 gene was identified in five strains of Acinetobacter and one strain of Klebsiella pneumoniae. In addition, we detected seven isolates harboring the blaAFM-1 gene, a novel metallo-β-lactamase gene. This was first genomic analysis of ST11 Klebsiella pneumoniae co-producing NDM-5 and mcr-1, which revealed that blaNDM-5 and mcr-1 are located on two different plasmids. The plasmid harboring blaNDM-5, which was composed of a typical IncX3-type backbone, and the mcr-1 gene, was located between an IS30-like element ISApl1 and a PAP2-like encoding gene in the IncHI2-type plasmid. Conclusions: the overall prevalence of fecal carriage of CNS-GNB in 10,000 stool samples was 7.45% (745/10000), and CNS-GNB producing carbapenemase were up to 45.1% (307/680). Most CNS-GNB cases were associated with infectious disease, multiple hospitalizations, or long-term care, and a high prevalence of underlying disease.

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<p>Emergence of two <em>Escherichia coli</em> strains co-harboring <em>mcr-1</em> and bla<sub>NDM</sub> in fresh vegetables from China</p>

Cited by 28 publications

References 41 publications

Complete Genetic Analysis of Plasmids Carrying mcr-1 and Other Resistance Genes in Avian Pathogenic Escherichia coli Isolates from Diseased Chickens in Anhui Province in China

Complete Genetic Analysis of Plasmids Carrying mcr-1 and Other Resistance Genes in Avian Pathogenic Escherichia coli Isolates from Diseased Chickens in Anhui Province in China

Co-Occurrence of NDM-9 and MCR-1 in a Human Gut Colonized Escherichia coli ST1011

Characterization of carbapenem non-susceptible Gram-negative Bacilli isolated from the feces of 10,000 inpatients in Southern China

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