<p>Elevation in the Expression of circ_0079586 Predicts Poor Prognosis and Accelerates Progression in Glioma via Interactions with the miR-183-5p/<em>MDM4</em> Signaling Pathway</p>

Abstract

Purpose: Glioma (GM) usually presents with an aggressive behavior and has a poor survival outcome. The abnormal expression of circular RNAs (circRNAs) has already been detected in GM, and circ_0079586 was found to have an increased expression in GM tumors. Patients and Methods: We assessed the differences in the expression of circ_0079586 in GM tissues (N=60) and cell lines (N=5) using qRT-PCR. The clinical value of circ_0079586 was measured by Fisher's exact test and Kaplan-Meier and Cox regression analyses. Circ_0079586 siRNA and vector were transfected into LN229 and U251 cells, respectively, and the transfection was verified by qRT-PCR. Cell growth was evaluated by cell counting kit-8 (CCK-8). Cell apoptosis was measured using flow cytometric assay. Cell metastatic properties were measured by wound scratch and transwell experiments. Subcellular fractionation was used to identify the location of circ_0079586. Dual-luciferase reporter test was utilized to confirm the interaction between miR-183-5p and circ_0079586/ MDM4 3ʹ-UTR. Results: The expression of circ_0079586 was elevated in GM samples and cells and correlated with the clinical severity and unfavorable prognosis of the patients. The elevated expression of circ_0079586 led to an increase in cell growth, migration and invasion but inhibited apoptosis in U251 cells, whereas its down-regulation reversed these effects in the LN229 cells. Mechanistically, we found circ_0079586 to be primarily located in the cytoplasm of GM cells. Furthermore, circ_0079586 could act as a sponge for miR-183-5p and elevate MDM4 expression at the posttranscriptional level. Conclusion: In summary, circ_0079586 was found to be up-regulated in GM that increased the proliferation, migration and invasion in GM cells via interaction with the miR-183-5p/ MDM4 axis. We anticipate that our study would provide newer insights into the mechanism and treatment of GM.

Mediation of circ_RPPH1 on miR-146b-3p/E2F2 pathway to hinder the growth and metastasis of breast carcinoma cells

“…For example, [ 29 ] circTADA2As hinders the progression and metastasis of BC by targeting miR-203a-3p/SOCS3 axis. Other studies found that the enhanced circ_0079586 can predict the poor prognosis of glioma and accelerate its progression through the interaction with miR-183-5p/MDM4 [ 30 ]. In order to verify that circ_RPPH1 has the effect as ceRNA, we first localized it.…”

CircRNA_0079586 and circRNA_RanGAP1 are involved in the pathogenesis of intracranial aneurysms rupture by regulating the expression of MPO

“…Several circRNAs have been reported to be dysregulated in human endothelial cells 27 , 28 . Specially, circRNA_0079586 was found to accelerate the progression of glioma via interacting with miR-183-5p, while circRNA_RanGAP1 was found to facilitate gastric cancer invasion and metastasis via interacting with miR-877-3p 29 , 30 . Moreover, previous reports demonstrated that MPO not only contributed to the formation and rupture of cerebral aneurysm, but was also correlated with the degenerative remodeling predisposition to saccular intracranial aneurysm wall rupture 22 , 31 .…”