<p>Effect of Intracorporeal Human Adipose–Derived Stem Cells (hADSCs) on Corpora Cavernosa Transforming Growth Factor β1 (TGFβ1) and Collagen Type I Concentration in Wistar Rat Priapism Model</p>

Abstract

Introduction: The ischemic process in priapism can lead to displacement of normal tissue with fibrotic tissue, due to collagen deposition, and eventually leads to erectile dysfunction. Many studies have identified that the supernatant of adipose tissue-derived stem cells (ADSCs) significantly ameliorates fibrosis of different tissue, but limited attention has been paid to its efficacy on fibrosis of the corpora cavernosa. Methods: A total of 22 Wistar rats divided into five groups, with two groups each consisting of five male wistar rats with priapism without human ADSC (hADSC) therapy (group I) and two other groups consisting of five rats with priapism, were given 10 6 cells' intracorporeal hADSC injection after 12 hours of penile clamping (group II) were euthanized after 2 and 4 weeks of observation. The last group consisted of two rats without any treatment or model (group III). Following euthanasia, penises were harvested for TGFβ 1 and collagen type I measurement using ELISA. Statistical analysis using independent-sample t-tests was done with SPSS 21.0. Results: Penile TGFβ 1 concentration in the treatment group was significantly lower in the second and fourth weeks of observation (p 2 =0.004, p 4 =0.003), and collagen type I was significantly lower in the second and fourth weeks (p 2 =0.003, p 4 =0.011). Conclusion: Intracorporeal hADSC injection limited the fibrosis process in a priapism model. Although the mechanism was unclear, it may be related to the potential of hADSCs to produce various growth factors that could limit TGFβ 1 and collagen production.

Transplantation of adipose-derived stem cells ameliorates Echinococcus multilocularis-induced liver fibrosis in mice

“…In recent years, anti-fibrotic mechanisms of ADSCs have been progressively elucidated [18,19]. TGF-β is one of the most classical and important signaling pathway in fibrosis pathogenesis, and its up-regulation activates hepatic stellate cells (HSCs), α-smooth muscle actin (α-SMA) expression, and the synthesis of ECM proteins, such as collagens [20].…”

The role of red ginseng in men’s reproductive health: a literature review

“…With regard to improving erectile function in ED rats with metabolic syndrome, penile cavernous fibrosis progresses in vascular-derived ED. In addition, the expression of transforming growth factor-β 1 (TGF-β1), a molecular marker of fibrosis in fractionated vascular ED, induces damaged-tissue repair by aggregating fibroblasts in areas of ischemic-tissue damage and promoting the production of collagenous connective tissue [ 88 ]. In the study by Kim et al [ 52 ], the proportion of penile smooth muscle cells in rats suffering from metabolic syndrome was significantly reduced, whereas that with RG treatment was close to that of normal controls and inhibited TGF-β1 expression in the penile corpus cavernosum (Fig.…”

Refinement of Animal Model of Colorectal Carcinogenesis through the Definition of Novel Humane Endpoints

“…However, in our study, no animal exhibited such an advance neoplastic disease, and the decrease in BW might be associated with DMH proinflammatory effects. Priapism is a pathological condition characterized by a prolonged erection (about four hours or more), which is usually painful and is not due to any sexual stimulation [ 30 , 31 ]. Neurological and metabolic diseases, as well as the excessive use of drugs, have been associated with this condition [ 30 ].…”