&lt;p&gt;Circular RNA CircPRMT5 Accelerates Proliferation and Invasion of Papillary Thyroid Cancer Through Regulation of miR-30c/E2F3 Axis&lt;/p&gt;

Xue, Cheng; Cheng, Yi; Wu, Jinyou; Ke, Kongliang; Miao, Chundi; Chen, Enfu; Zhang, Luqing

doi:10.2147/cmar.s249237

Cited by 24 publications

(20 citation statements)

References 27 publications

(33 reference statements)

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“…Besides, circPRMT5 could regulate E2F3 expression, and then improve invasion and proliferation of PRC. 27 Above evidences were consistent with our results, siE2F3 could rescue effects of miR-153-3p inhibitor in thyroid cancer development. Besides, Ki67 is one of the most widely studied indicators of cell proliferation, which is involved in the occurrence and development of tumors.…”

Section: Discussionsupporting

confidence: 90%

MiR-153-3p Suppresses Cell Proliferation, Invasion and Glycolysis of Thyroid Cancer Through Inhibiting E3F3 Expression

Deng

Guo

Fan

2021

OTT

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The aim was to research the role of miR-153-3p and E2F3 in the development of thyroid tumors. Methods: A total of 91 thyroid cancer patients were involved. The role of miR-153-3p in THCA cell lines and Nthy-ori3-1 cell line was researched. qPCR was used to detect miR-153-3p and E2F3 expression. MiR-153-3p mimic, inhibitor, siE2F3 or corresponding controls were transfected in cells. CCK8 was used to verify the proliferation. Cell cycle and apoptosis was detected by flow cytometry. Transwell assay was applied for migration and invasion, and glycolysis was monitored. The binding of miR-153-3p and E2F3 was predicted by targetscan database, and verified by luciferase reporter and RNA-pull down assay. Western blot was used to detect E2F3 expression. Rescue assay was undertaken to verify the effect of siE2F3 on miR-153-3p inhibitor. Moreover, the effect of miR-153-3p mimic on tumor volume and weight was measured. IHC assay was processed to E2F3 and Ki67 expression, and TUNEL assay was used for apoptosis. Results: MiR-153-3p expressed lower in thyroid tumors and cells. The level of miR-153-3p was negatively related with TNM stage. MiR-153-3p inhibited cell proliferation, invasion migration, and induced cycle arrest and apoptosis. Moreover, it negatively regulated E2F3. siE2F3 rescued effects of miR-153-3p inhibitor in all above biological processes in thyroid cancer cells. MiR-153-3p inhibited tumor growth. Moreover, it inhibited E2F3 and Ki67 expression, and also increased apoptosis in vivo. Conclusion: MiR-153-3p suppresses cell proliferation, invasion and glycolysis of thyroid cancer through inhibiting E3F3 expression, which may be a biomarker for thyroid cancer diagnose.

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Section: Discussionsupporting

confidence: 90%

MiR-153-3p Suppresses Cell Proliferation, Invasion and Glycolysis of Thyroid Cancer Through Inhibiting E3F3 Expression

Deng

Guo

Fan

2021

OTT

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“…TPC-1 and KTC-1 cells (6 × 10 4 cells/well) were incubated in 24-well plates for 24 h. A luciferase reporter vectors containing circ007293-miR-653-5p or PAX6 mRNA 3ʹUTR-miR-653-5p binding sequences (circ007293-WT or PAX6-WT) or mutant sequences (circ007293-MUT or PAX6-MUT) and miR-653-5p mimic or miR-NC were co-transfected using Lipofectamine 2000 (Thermo Fisher Scientific). Luciferase activity was assessed with the Dual Luciferase Reporter Assay System (Promega, Madison, WI, USA) for 48 h following instructions stipulated by the manufacturer [ 24 ].…”

Section: Transwell Assaymentioning

confidence: 99%

Exosomal circular RNA hsa_circ_007293 promotes proliferation, migration, invasion, and epithelial–mesenchymal transition of papillary thyroid carcinoma cells through regulation of the microRNA-653-5p/paired box 6 axis

Lin

Hou

et al. 2021

Bioengineered

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Circular RNAs (circRNAs) or exosomes have been reported to exert key regulatory and/or communication functions in human cancer. Nevertheless, current literature on the effects of exosomal circRNAs on tumor invasion and metastasis in thyroid cancer is incomplete. The role of tumor-derived exosomes in driving in vitro papillary thyroid carcinoma (PTC) progression and metastasis requires further investigation. In our study, Exosomes were harvested from PTC patient serum and PTC cell culture medium. Gene expression analysis in PTC cell lines and exosomes was performed with quantitative reverse-transcription polymerase chain reaction. Transwell, wound healing, Western blot assays, and the cell counting kit-8 were applied for functional analysis. Dual-luciferase reporter assay was used to examine the interaction between hsa_circ_007293 (circ007293), microRNA (miR)-653-5p, and paired box 6 (PAX6). Results showed that circ007293 was enriched in exosomes derived from PTC patient serum and cell culture media. Moreover, circ007293 could enter PTC cells through exosomes, and exosomal circ007293 promoted PTC cell epithelial–mesenchymal transition, invasion, migration, and proliferation. circ007293 knockdown reversed the malignant phenotype of PTC cells in vitro . Additionally, circ007293 could competitively bind with miR-653-5p to regulate PAX6 expression. Notably, miR-653-5p overexpression or PAX6 inhibition suppressed the malignant effects of exosomal circ007293. These results evidenced that exosomal circ007293 induced EMT and augmented the invasive and migratory abilities of PTC cells via the miR-653-5p/PAX6 axis, suggesting that it may serve as a promising biomarker for cancer progression.

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“…28 E2F3 has been reported as an oncogene in many cancers by many studies. Xue et al 29 showed that circ-PRMT5 could accelerate proliferation and invasion of papillary thyroid cancer through regulation of the miR-30c/E2F3 axis. Huang et al 30 reported that E2Fs could worked as promising diagnostic and prognostic markers in human hepatocellular carcinoma.…”

Section: Discussionmentioning

confidence: 99%

E2F3 promotes liver cancer progression under the regulation of circ-PRKAR1B

Liu

Ouyang

Gong

et al. 2021

Molecular Therapy - Nucleic Acids

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Liver cancer is one of the most lethal malignant tumors in the world. The high recurrence and mortality rate make it urgent for scientists and clinicians to find new targets for better treatment of liver cancer. Here, we found that circ-PRKAR1B expression was increased in the paired intrahepatic metastasis sample through high-throughput sequencing. Further experiments also confirmed its high expression both in carcinoma and metastasis when compared to the paired para-carcinoma and the paired carcinoma, respectively. Mechanism study showed that circ-PRKAR1B could promote liver cancer progression through the miR-432-5p/E2F3 pathway, and micro-RNA-432-5p could directly target the 3 0 untranslated region (UTR) of E2F3 mRNA to suppress its translation, thereby influencing liver cancer cell invasion and migration capacities. Clinical data obtained by using online databases based on The Cancer Genome Atlas (TCGA) samples and the clinicopathological data of liver cancer patients who underwent surgery in our hospital in the past 2 years also confirmed the significance of circ-PRKAR1B/miR-432-5p/E2F3 signaling in liver cancer progression. Animal experiments also indicated that targeting this newly identified signaling by overexpressing microRNA-432-5p could suppress the progression of liver cancer. Together, our study suggests that circ-PRKAR1B plays an important role in the regulation of liver cancer progression, and targeting this new circ-PRKAR1B/miR-432-5p/E2F3 signaling may help us find new treatment strategies to better suppress liver cancer progression.

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<p>Circular RNA CircPRMT5 Accelerates Proliferation and Invasion of Papillary Thyroid Cancer Through Regulation of miR-30c/E2F3 Axis</p>

Cited by 24 publications

References 27 publications

MiR-153-3p Suppresses Cell Proliferation, Invasion and Glycolysis of Thyroid Cancer Through Inhibiting E3F3 Expression

MiR-153-3p Suppresses Cell Proliferation, Invasion and Glycolysis of Thyroid Cancer Through Inhibiting E3F3 Expression

Exosomal circular RNA hsa_circ_007293 promotes proliferation, migration, invasion, and epithelial–mesenchymal transition of papillary thyroid carcinoma cells through regulation of the microRNA-653-5p/paired box 6 axis

E2F3 promotes liver cancer progression under the regulation of circ-PRKAR1B

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