<p>Cell penetrating peptide-modified nanoparticles for tumor targeted imaging and synergistic effect of sonodynamic/HIFU therapy</p>

Abstract

Background Theranostics based on multifunctional nanoparticles (NPs) is a promising field that combines therapeutic and diagnostic functionalities into a single nanoparticle system. However, the major challenges that lie ahead are how to achieve accurate early diagnosis and how to develop efficient and noninvasive treatment. Sonodynamic therapy (SDT) utilizing ultrasound combined with a sonosensitizer represents a novel noninvasive modality for cancer therapy. Different ultrasound frequencies have been used for SDT, nevertheless, whether the effect of SDT can enhance synergistic HIFU ablation remains to be investigated. Materials and methods We prepared a nanosystem for codelivery of a sonosensitizer (methylene blue, MB) and a magnetic resonance contrast agent (gadodiamide, Gd-DTPA-BMA) based on hydrophilic biodegradable polymeric NPs composed of poly (lactic-co-glycolic acid) (PLGA). To enhance accumulation and penetration of the NPs at the tumor site, the surface of PLGA NPs was decorated with a tumor-homing and penetrating peptide-F3 and polyethylene glycol (PEG). The physicochemical, imaging and therapeutic properties of F3-PLGA@MB/Gd and drug safety were thoroughly evaluated both in vitro and in vivo. F3-PLGA@MB/Gd was evaluated by both photoacoustic and resonance imaging. Results F3-PLGA@MB/Gd NPs exhibited higher cellular association than non-targeted NPs and showed a more preferential enrichment at the tumor site. Furthermore, with good drug safety, the apoptosis triggered by ultrasound in the F3-PLGA@MB/Gd group was greater than that in the contrast group. Conclusion F3-PLGA@MB/Gd can work as a highly efficient theranostic agent, and the incorporation of targeted multimodal and combined therapy could be an encouraging strategy for cancer treatment.

Cell Penetrating Peptides: Applications in Tumor Diagnosis and Therapeutics

“…post-injection. Again, this study highlights a promising CPP (F3) and CPP-NP combination for tumor imaging, demonstrating that there are multiple variations of CPPs and combinations with CPPs that can be used to advance tumor imaging 41 .…”

Recent progress in sono-photodynamic cancer therapy: From developed new sensitizers to nanotechnology-based efficacy-enhancing strategies

“…In addition to iRGD peptide, F3 peptide that specifically targets nucleolin can also be used for mediating dual-targeting delivery. F3 peptide-modified nanoparticles were developed for targeted delivery to both tumor cells and tumor angiogenic endothelial cells to exert SDT effects in vitro under ultrasound 164 . Ce6-conjugated β -cyclodextrin and a designed peptide Ad-CGKRK-GFLG-EE-HAIYPRH (T7) could self-assemble into supramolecular micelles with dual targeting ability for enhanced PDT.…”

Microfluidic Preparation of Janus Microparticles With Temperature and pH Triggered Degradation Properties

“…It is well recognized that properties and applications of Janus particles are highly dependent on their morphology and chemical composition. Janus particles with adjustable size, biocompatibility, and low toxicity are widely used in biomedical fields, especially in the fields related to living cell research and tumor inhibition, such as cell encapsulation ( Zhao et al, 2009 ; Maeda et al, 2012 ), cell imaging ( Yi et al, 2016 ; Li et al, 2019 ), drug delivery ( Xuan et al, 2014 ; M. El-Sherbiny and Abbas, 2016 ; Feng et al, 2019 ; Severino et al, 2019 ) and so on. At present, Janus particles have covered the whole range from nano scale to micron scale.…”