&lt;p&gt;Anti-Inflammatory Activity of Sanjie Zhentong Capsule Assessed By Network Pharmacology Analysis of Adenomyosis Treatment &lt;/p&gt;

Du, Li; Du, Dehui; Chen, Biao; Ding, Yue; Zhang, Tong; Xiao, Wei

doi:10.2147/dddt.s228721

Cited by 11 publications

(8 citation statements)

References 63 publications

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“…According to the theory of TCM, "blood stasis" is the core pathogenesis of AM (Bi, 2020), resulting in the progressive exacerbation of dysmenorrhea in AM (Imanaka et al, 2021;Alcalde et al, 2022). TCM for promoting blood circulation has been shown to alleviate AM dysmenorrhea symptoms, reduce menstrual volume, and lower CA125 levels (Du and Du, 2020) (Zi et al, 2020) (Wang H Y et al, 2018). Dahuang -Taoren (DT), a classic botanical drug combination to combat "blood stasis," is clinically used for AM treatment in China.…”

Section: Discussionmentioning

confidence: 99%

Dahuang—Taoren, a botanical drug combination, ameliorates adenomyosis via inhibiting Rho GTPases

Lei

Fu²,

Chen³

et al. 2023

Front. Pharmacol.

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Introduction: Dahuang-Taoren (DT) is a classic combination of botanical drugs applied to treat pain-related diseases in ancient China. Today, DT is frequently applied for dysmenorrhea of adenomyosis (AM) in the clinic. Growing evidence indicates Rho GTPases may play an essential role in AM progression. However, the potential mechanism of DT on Rho GTPases in AM remains unclear.Methods: The expressions of Rho GTPases in the patients with AM were evaluated. Further, pituitary transplantation-induced AM mice and the primary AM endometrial stromal cells (AMESCs) were subjected to DT intervention.Results: The results revealed that the expressions of Rho GTPases were significantly upregulated in both AM patients and AM mice. The DT could reduce pathological infiltration, relieve hyperalgesia, and alleviate cytoskeleton remodeling in AM mice. Besides, the migration and invasion of AMESCs were markedly inhibited after exposure to DT.Discussion: These effects may be linked to the decreased Rho GTPases expression. The results may offer a novel explanation of DT against AM.

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Section: Discussionmentioning

confidence: 99%

Dahuang—Taoren, a botanical drug combination, ameliorates adenomyosis via inhibiting Rho GTPases

Lei

Fu²,

Chen³

et al. 2023

Front. Pharmacol.

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“…The efficacy of TCM in treating AM has been confirmed through several hundred years' practices and experimental research [ 11 ]. Previous studies have found that TCM treatment can alleviate dysmenorrhea, shorten the menstrual time, decrease menstrual volume, reduce uterine volume, and lower CA125 levels in AM patients [ 12 – 14 ]. GZFLW is one of the classic traditional Chinese herbal formulas widely used in the clinical treatment of AM [ 8 ], and studies have confirmed that the side effect of GZFLW on AM is only 1.99% [ 15 ].…”

Section: Discussionmentioning

confidence: 99%

Integrating Network Pharmacology and Experimental Validation Deciphers the Mechanism of Guizhi Fuling Wan against Adenomyosis

Wang

Zhang

Zhu

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Aim. This study aimed to predict the key targets and endocrine mechanisms of Guizhi Fuling Wan (GZFLW) in treating adenomyosis (AM) through network pharmacology, molecular docking, and animal experiment verification. Methods. The related ingredients and targets of GZFLW in treating AM were screened out using TCMSP, BATMAN-TCM, SwissTargetPrediction, and PubChem Database. Then, the protein-protein interaction (PPI) analysis and the network of compound-hub targets were constructed. At the same time, the key targets were uploaded to the Metascape Database for KEGG pathway enrichment analysis. After that, the molecular docking technology of the main active components and hub targets was performed. Furthermore, animal experiments were used to verify the results of network pharmacology analysis. Results. A total of 55 active ingredients of GZFLW and 44 overlapping targets of GZFLW in treating AM were obtained. After screening, 25 hub targets were collected, including ESR1, EGF, and EGFR. Then, the KEGG pathway enrichment analysis results indicated that the endocrine therapeutic mechanism of GZFLW against AM is mainly associated with the estrogen signaling pathway, endocrine resistance, and an EGFR tyrosine kinase signaling pathway. Then, molecular docking showed that the significant compounds of GZFLW had a strong binding ability with ERα and EGFR. More importantly, the animal experiments confirmed that the GZFLW could downregulate the abnormal infiltration of the endometrial epithelium into the myometrium and had no interference with the normal sexual cycle. This effect may be directly related to intervening the local estrogen signaling pathway of the endometrial myometrial interface (EMI). It may also be associated with the myometrium cells’ estrogen resistance via GPER/EGFR signaling pathway. Conclusion. The endocrine mechanism of GZFLW in treating AM was explored based on network pharmacology, molecular docking, and animal experiments, which provided a theoretical basis for the clinical application of GZFLW.

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“…[ 18 ] Cytoscape3.8.0 (http://www.cytoscape.org/) was used for analysis to construct the component‐target‐disease network. [ 19–21 ]…”

Section: Methodsmentioning

confidence: 99%

“…cytoscape.org/) was used for analysis to construct the componenttarget-disease network. [19][20][21] Gene pathway analysis: The DAVID (the Database for Annotation, Visualization, and Integrated Discovery) database (http://david. abcc.ncifcrf.gov/) is an online, freely available bioinformatics database for differential gene functional and pathway enrichment analysis.…”

Section: Network Pharmacology Studiesmentioning

confidence: 99%

Network pharmacology and molecular docking integrated strategy to investigate the pharmacological mechanism of palmatine in Alzheimer's disease

Pei

Zeng

Chen

et al. 2022

J Biochem & Molecular Tox

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Objective The key molecular mechanism of palmatine in the treatment of Alzheimer's disease (AD) was investigated in this article. Methods Network pharmacology techniques constructed drug‐target‐disease relationship networks and predictive pathways of action. At the cellular level, lipopolysaccharide (LPS) was used to induce Raw 264.7 cells to establish an inflammation model, and interleukin (IL)‐1β, IL‐6, and tumor necrosis factor (TNF)‐α indicators were examined. Apoptosis was detected using Hoechst 33258. At the animal level, LPS was used to induce AD animal model, and behavioral performance were examined by water maze, and serum biochemical indexes were measured by ELISA. And the expression of PI3K and P‐AKT was observed by immunohistochemistry. Finally, molecular level validation was performed using the molecular docking technique. Results The result of Network pharmacological was predicted that palmatine may treat AD mainly through the PI3K pathway. Palmatine has no significant effect on Raw264.7 cells viability within 0.05 mg/ml, Palmatine can significantly induce Raw264.7 cells to secret IL‐6 and IL1‐β in a concentration‐dependent manner, but it has not obvious impact on NO and TNF‐α. Palmatine has a significant restorative effect on the cell viability of Raw264.7 in a concentration of 0.1 mg/ml. Palmatine can be concentration‐dependent to downregulate the secretion of LPS‐induced IL‐6. At the same time, Palmatine also has a significant effect on the level of TNF‐α induced by LPS, it also can slightly downregulate the secretion of IL‐1β. The results of Hoechst33258 showed that cells in the 0.025 mg/ml and 0.5 mg/ml delivery groups increased with different degrees of bright blue fluorescence, and apoptosis rate decreased. Animal experiments showed that palmatine effectively improved the learning and memory ability of AD mice. The immunohistochemical results exhibited that the expression of PI3K and P‐AKT in the model group decreased, but they were obvious reversed by palmatine The molecular docking results showed that palmatine and key targets had good docking, among which the binding to ERBB2, CDC42, MDM2, and mTOR was the most likely. Conclusion Palmatine has neuroprotective effects. Palmatine could effectively ameliorate memory impairment in AD mice by promoting the PI3K‐AKT pathway. Molecular docking results showed that palmatine has a better binding ability with mTOR.

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<p>Anti-Inflammatory Activity of Sanjie Zhentong Capsule Assessed By Network Pharmacology Analysis of Adenomyosis Treatment </p>

Cited by 11 publications

References 63 publications

Dahuang—Taoren, a botanical drug combination, ameliorates adenomyosis via inhibiting Rho GTPases

Dahuang—Taoren, a botanical drug combination, ameliorates adenomyosis via inhibiting Rho GTPases

Integrating Network Pharmacology and Experimental Validation Deciphers the Mechanism of Guizhi Fuling Wan against Adenomyosis

Network pharmacology and molecular docking integrated strategy to investigate the pharmacological mechanism of palmatine in Alzheimer's disease

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