&lt;p&gt;Anesthetics and human epidermal growth factor incorporated into anti-adhesive nanofibers provide sustained pain relief and promote healing of surgical wounds&lt;/p&gt;

Kao, Ching-Wei; Tseng, Yuan Yun; Liu, Kuo‐Sheng; Liu, Yen-Wei; Chen, Jin-Chung; He, Hong-Lin; Kau, Yi-Chuan; Liu, Shih‐Jung

doi:10.2147/ijn.s202402

Cited by 23 publications

(14 citation statements)

References 21 publications

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“…In addition, vitamin C maintained its ability to facilitate secretion of type I collagen by fibroblasts, EGF stimulated proliferation of skin fibroblasts, and insulin potentiated adipogenic differentiation of fibroblasts [11]. In PLGA nanofibers, EGF was also combined with the local anesthetic lidocaine in order to accelerate wound healing in a rat model [89]. Coating PLGA nanofibers with a self-assembled complex of poly(ethylene argininyl aspartate diglyceride) polycation, heparin, and cargo growth factors, i.e., vascular endothelial growth factor (VEGF) and/or transforming growth factor-beta3 (TGF-β3), enhanced proliferation of human dermal fibroblasts and formation of tubular structures from human umbilical vein endothelial cells in vitro.…”

Section: Nanofibers From Synthetic Degradable Polymersmentioning

confidence: 99%

Nanofibrous Scaffolds for Skin Tissue Engineering and Wound Healing Based on Synthetic Polymers

Bačáková¹,

Zikmundová²,

Pajorová³

et al. 2020

Applications of Nanobiotechnology

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Nanofibrous scaffolds are popular materials in all areas of tissue engineering, because they mimic the fibrous component of the natural extracellular matrix. In this chapter, we focused on the application of nanofibers in skin tissue engineering and wound healing, because the skin is an organ with several vitally important functions, particularly barrier, thermoregulatory, and sensory functions. Nanofibrous meshes not only serve as carriers for skin cells but also can prevent the penetration of microbes into wounds and can keep appropriate moisture in the damaged skin. The nanofibrous meshes have been prepared from a wide range of synthetic and nature-derived polymers. This review is concentrated on synthetic non-degradable and degradable polymers, which have been explored for skin tissue engineering and wound healing. These synthetic polymers were often combined with natural polymers of the protein or polysaccharide nature, which improved their attractiveness for cell colonization. The nanofibrous scaffolds can also be loaded with various bioactive molecules, such as growth factors, hormones, vitamins, antioxidants, antimicrobial, and antitumor agents. In advanced tissue engineering approaches, the cells on the nanofibrous scaffolds are cultured in dynamic bioreactors enabling appropriate mechanical stimulation of cells and at air-liquid interface. This chapter summarizes recent results achieved in the field of nanofiber-based skin tissue engineering, including results of our research group.

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Section: Nanofibers From Synthetic Degradable Polymersmentioning

confidence: 99%

Nanofibrous Scaffolds for Skin Tissue Engineering and Wound Healing Based on Synthetic Polymers

Bačáková¹,

Zikmundová²,

Pajorová³

et al. 2020

Applications of Nanobiotechnology

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“…To manufacture the nanofibrous membranes, PLGA and doxycycline were primarily dissolved in HFIP at concentrations of 30% (w/v) and 7.5% (w/v) respectively. 26,27 Electrospinning of the PLGA/doxycycline solution was performed on a lab-made setup consisting of a syringe and needle (the internal diameter is 0.42 mm), a ground collection plate, and a high voltage supply. The flow rate of the solution was 0.1 mL/h, while the applied voltage was 15 kV.…”

Section: Preparation Of Doxycycline-incorporated Nanofibrous Membranesmentioning

confidence: 99%

<p>Doxycycline-Embedded Nanofibrous Membranes Help Promote Healing of Tendon Rupture</p>

Weng¹,

Lee²,

Ho³

et al. 2020

IJN

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Background: Despite recent advancements in surgical techniques, the repair of tendon rupture remains a challenge for surgeons. The purpose of this study was to develop novel doxycycline-loaded biodegradable nanofibrous membranes and evaluate their efficacy for the repair of Achilles tendon rupture in a rat model. Materials and Methods: The drug-loaded nanofibers were prepared using the electrospinning process and drug release from the prepared membranes was investigated both in vitro and in vivo. Furthermore, the safety and efficacy of the drug-loaded nanofibrous membranes were evaluated in rats that underwent tendon surgeries. An animal behavior cage was employed to monitor the post-surgery activity of the animals. Results: The experimental results demonstrated that poly(D,L-lactide-co-glycolide) (PLGA) nanofibers released effective concentrations of doxycycline for more than 40 days post-surgery, and the systemic plasma drug concentration was low. Rats receiving implantation of doxycycline-loaded nanofibers also showed greater activities and stronger tendons post-operation. Conclusion: Nanofibers loaded with doxycycline may have great potential in the repair of Achilles tendon rupture.

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“… 16 Kao et al exploited analgesics-incorporated anti-adhesive membranes to offer sustainable elution of lidocaine and ketorolac to relieve postoperative pain. 17 , 18 Khan et al 19 proposed that the laser-mediated surface activation of graphene oxide offers high efficiency for antifungal and antibacterial. Hussein et al 20 developed ultrasonicated graphene oxide that provides a good environment for cells involved in bone and skin healing.…”

Section: Introductionmentioning

confidence: 99%

Assessment of Antimicrobial Agents, Analgesics, and Epidermal Growth Factors-Embedded Anti-Adhesive Poly(Lactic-Co-Glycolic Acid) Nanofibrous Membranes: In vitro and in vivo Studies

Liu¹,

Kao²,

Tseng³

et al. 2021

IJN

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Background Postoperative tissue adhesion is a major concern for most surgeons and is a nearly unpreventable complication after abdominal or pelvic surgeries. This study explored the use of sandwich-structured antimicrobial agents, analgesics, and human epidermal growth factor (hEGF)-incorporated anti-adhesive poly(lactic-co-glycolic acid) nanofibrous membranes for surgical wounds. Materials and Methods Electrospinning and co-axial electrospinning techniques were utilized in fabricating the membranes. After spinning, the properties of the prepared membranes were assessed. Additionally, high-performance liquid chromatography and enzyme-linked immunosorbent assays were utilized in assessing the in vitro and in vivo liberation profiles of the pharmaceuticals and the hEGF from the membranes. Results The measured data suggest that the degradable anti-adhesive membranes discharged high levels of vancomycin/ceftazidime, ketorolac, and hEGF in vitro for more than 30, 24, and 27 days, respectively. The in vivo assessment in a rat laparotomy model indicated no adhesion in the peritoneal cavity at 14 days post-operation, demonstrating the anti-adhesive capability of the sandwich-structured nanofibrous membranes. The nanofibers also released effective levels of vancomycin, ceftazidime, and ketorolac for more than 28 days in vivo. Histological examination revealed no adverse effects. Conclusion The outcomes of this study implied that the anti-adhesive nanofibers with sustained release of antimicrobial agents, analgesics, and growth factors might offer postoperative pain relief and infection control, as well as promote postoperative healing of surgical wounds.

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<p>Anesthetics and human epidermal growth factor incorporated into anti-adhesive nanofibers provide sustained pain relief and promote healing of surgical wounds</p>

Cited by 23 publications

References 21 publications

Nanofibrous Scaffolds for Skin Tissue Engineering and Wound Healing Based on Synthetic Polymers

Nanofibrous Scaffolds for Skin Tissue Engineering and Wound Healing Based on Synthetic Polymers

<p>Doxycycline-Embedded Nanofibrous Membranes Help Promote Healing of Tendon Rupture</p>

Assessment of Antimicrobial Agents, Analgesics, and Epidermal Growth Factors-Embedded Anti-Adhesive Poly(Lactic-Co-Glycolic Acid) Nanofibrous Membranes: In vitro and in vivo Studies

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