<p>A chemoenzymatically synthesized cholesterol-g-poly(amine-co-ester)-mediated <em>p53</em> gene delivery for achieving antitumor efficacy in prostate cancer</p>

Abstract

Background: An amphiphilic cationic copolymer cholesterol-g-poly(amine-co-ester), namely Chol-g-PMSC-PPDL synthesized in a chemoenzymatic route has been utilized as a carrier for p53 gene delivery to check its antitumor efficacy, using human prostate cancer cell line PC-3 (p53 null) as a model. Materials and methods: The transfection efficiency was measured by quantitative PCR and Western blotting assay. The anti-proliferative effect was detected using MTT method, colony formation assay and Live/Dead staining. The anti-migration effect was evaluated through wound healing and Transwell migration assays. Results: The transfection efficiency assay indicated that the carrier-mediated p53 gene transfection could dramatically enhance the intracellular p53 expression level. Through p53 gene delivery, obvious anti-proliferative effect could be detected which was elucidated to be associated with the simultaneous activation of mitochondrial-dependent apoptosis pathway and cell cycle arrest at G1 phase. Meanwhile, the anti-migration effect could be obtained after p53 gene transfection. Conclusion: Chol-g-PMSC-PPDL-mediated p53 gene transfection could potentially be employed as a promising strategy for achieving effective anti-tumor response.

<p>Inhibition of proliferation and migration of tumor cells through phenylboronic acid-functionalized polyamidoamine-mediated delivery of a therapeutic DNAzyme Dz13</p>

“…Additionally, the expression levels of procaspase-8 and −9 decreased after the carriers-mediated Dz13 transfection, suggesting the activation of caspase-8 and −9 through the cleavage of their precursors. Basically, the activation of caspase-8 has been identified to play a critical role in the cell apoptosis through the death receptor-dependent pathway, while caspase-9 could initiate the cell apoptosis based on the mitochondria-dependent apoptotic pathway 39,40. Thus, we inferred that the carrier-mediated Dz13 transfection could trigger the cell apoptosis through both death receptor-dependent pathway and mitochondrial apoptotic signaling pathway.…”

Baicalin-modified polyethylenimine for miR-34a efficient and safe delivery

“…In addition, the alteration of pro-caspase-8 and pro-caspase-9 were decreased after BA-PEI/miR-34a transfection, indicating that caspase-8 and caspase-9 were activated by the cleavage of their precursors. Fundamentally, it has been established that caspase-8 is activated based on death receptor-dependent pathways and Frontiers in Bioengineering and Biotechnology frontiersin.org caspase-9 through mitochondrial pathways (Wang et al, 2018;Dong et al, 2019). Therefore, we conclude that BA-PEI/miR-34a may cure cancer through death receptor-dependent pathways and mitochondrial pathways and PTEN signaling pathways, which are well-studied tumor inhibition pathways (Jiang and Liu, 2009).…”

<p>Nano-Scaled Zeolitic Imidazole Framework-8 as an Efficient Carrier for the Intracellular Delivery of RNase A in Cancer Treatment</p>

“…Further, the cell survival was evaluated through Live/Dead staining assay, in which the dead cells emitted red fluorescence after the staining with ethidium homodimer while the viable cells generated green fluorescence due to the calcein AM staining 23,24. As shown in Figure S5 , dead cells could barely be observed in free RNase A-treating group while a large number of dead cells could be clearly detected after the treatment with RNase A@ZIF-8 nanoparticles.…”

“…The cells were treated with free RNase A, ZIF-8, and RNase A@ZIF-8 nanoparticles harboring 10 μg/mL RNase A for 48 hrs. According to the manufacturer’s instructions, the cells were treated with live/dead staining reagent for 30 mins, in which dead and viable cells were stained with ethidium homodimer and calcein AM, respectively 23,24. After washing with PBS three times, the cells were observed through an IX71 fluorescence microscopy (Olympus, Tokyo, Japan).…”