&lt;p&gt;A Chelate-Free Nano-Platform for Incorporation of Diagnostic and Therapeutic Isotopes&lt;/p&gt;

Gholami, Yaser Hadi; Josephson, Lee; Akam, Eman A.; Caravan, Peter; Wilks, Moses Q.; Pan, Xiang-Zuo; Maschmeyer, Richard; Kolnick, Aleksandra; Fakhri, Georges El; Normandin, Marc D.; Kuncic, Zdenka; Yuan, Haidong

doi:10.2147/ijn.s227931

Cited by 9 publications

(15 citation statements)

References 77 publications

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“…Heat-Induced Radiolabeling (HIR) HIR was carried out according to the published protocol. 7,10 Briefly, three identical FH samples were prepared, two for radiochemical analysis and one for phantom imaging. For each sample, the reaction mixture was prepared by adding 20 µL [ 89 Zr]Zr (m Zr ≈ 0.0125 nmol, N atoms ≈ 7.5×10 12 , with A 0 ≈18.5 MBq) and 50 µL CTW into a 0.3 mL glass vial.…”

Section: Experimental Methodsmentioning

confidence: 99%

“…This is also consistent with our previous study. 7 The inductively coupled plasma mass spectrometry analysis of a range of HIR labeled metal-FH products demonstrated that all of Sr 2+ , Ba 2+ , Mo 3+ , Pb 2+ , Cu 2+ , Bi 3+ , In 3+ , Y 3+ , Lu 3+ , Sm 3+ , Tb 3+ , and Eu 3+ cations were adsorb onto the FH surface via Radiocation Surface Adsorption. 7 The labeling yield (LY) is calculated using Equation (1), with the change in volume per FH particle,…”

Section: Transmission Electron Microscopy (Tem)mentioning

confidence: 99%

“…Therefore, cations with f-orbital electrons, more empty d-orbitals, larger radii, and higher positive charges (or higher oxidation state) result in a stronger electrostatic interaction and thus stronger bonding with the surface and a higher radiochemical yield (RCY). 7 In our recent publication, 7 we report on a method to optimize the HIR technique to further enhance the RCY by approximately 20% which could be applied in future work to further increase the RCY of radiolabeling FH SPIONs with 89 Zr.…”

Section: Radiochemical Analysismentioning

confidence: 99%

“…Thus, FH-enhanced MR imaging can be used to non-invasively detect monocyte/macrophage trafficking and localize inflammatory lesions. 5,6 In our ongoing studies, [7][8][9] Feraheme was used as a nanoparticle platform to label a number of radio-isotopes commonly used in nuclear medicine, particularly Positron Emission Tomography (PET). Importantly, radiolabeling was achieved using a recently developed technique, heatinduced radiolabeling (HIR).…”

Section: Introductionmentioning

confidence: 99%

“…Importantly, radiolabeling was achieved using a recently developed technique, heatinduced radiolabeling (HIR). 7,10 The technique is chelatefree and is thus more favorable for clinical applications than conventional labeling methods that rely on different chemical chelators unstable and/or alter nanoparticle biodistribution in vivo. 11,12 The motivation for radiolabeling FH with PET tracers is to take advantage of multi-modal PET-MR imaging technology, which is proving invaluable in a growing number of clinical imaging applications.…”

Section: Introductionmentioning

confidence: 99%

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<p>A Radio-Nano-Platform for T1/T2 Dual-Mode PET-MR Imaging</p>

Gholami

Yuan

Wilks

et al. 2020

IJN

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Video abstractPoint your SmartPhone at the code above. If you have a QR code reader the video abstract will appear. Or use: http://youtu.be/Me_QBfX7I3sPurpose: This study aimed to develop a chelate-free radiolabeled nanoparticle platform for simultaneous positron emission tomography (PET) and magnetic resonance (MR) imaging that provides contrast-enhanced diagnostic imaging and significant image quality gain by integrating the high spatial resolution of MR with the high sensitivity of PET. Methods: A commercially available super-paramagnetic iron oxide nanoparticle (SPION) (Feraheme ® , FH) was labeled with the [ 89 Zr]Zr using a novel chelate-free radiolabeling technique, heat-induced radiolabeling (HIR). Radiochemical yield (RCY) and purity (RCP) were measured using size exclusion chromatography (SEC) and radio-thin layer chromatography (radio-TLC). Characterization of the non-radioactive isotope 90 Zr-labeled FH was performed by transmission electron microscopy (TEM). Simultaneous PET-MR phantom imaging was performed with different 89 Zr-FH concentrations. The MR quantitative image analysis determined the contrast-enhancing properties of FH. The signal-to-noise ratio (SNR) and full-width half-maximum (FWHM) of the line spread function (LSF) were calculated before and after coregistering the PET and MR image data. Results: High RCY (92%) and RCP (98%) of the [ 89 Zr]Zr-FH product was achieved. TEM analysis confirmed the 90 Zr atoms adsorption onto the SPION surface (≈ 10% average radial increase). Simultaneous PET-MR scans confirmed the capability of the [ 89 Zr]Zr-FH nanoplatform for this multi-modal imaging technique. Relative contrast image analysis showed that [ 89 Zr]Zr-FH can act as a dual-mode T1/T2 contrast agent. For co-registered PET-MR images, higher spatial resolution (FWHM enhancement ≈ 3) and SNR (enhancement ≈ 8) was achieved at a clinical dose of radio-isotope and Fe. Conclusion: Our results demonstrate FH is a highly suitable SPION-based platform for chelate-free labeling of PET tracers for hybrid PET-MR. The high RCY and RCP confirmed the robustness of the chelate-free HIR technique. An overall image quality gain was achieved compared to PET-or MR-alone imaging with a relatively low dosage of [ 89 Zr]Zr-FH. Additionally, FH is suitable as a dual-mode T1/T2 MR image contrast agent.

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Section: Experimental Methodsmentioning

confidence: 99%

Section: Transmission Electron Microscopy (Tem)mentioning

confidence: 99%

Section: Radiochemical Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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<p>A Radio-Nano-Platform for T1/T2 Dual-Mode PET-MR Imaging</p>

Gholami

Yuan

Wilks

et al. 2020

IJN

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Square‐wave Anodic Stripping Voltammetric Method for Novelty Detection of Bismuth Extracted by Aqueous Two‐phase Systems

et al. 2021

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In this work, a voltammetric sensor was used to monitor the concentration of bismuth extracted from an eutectic alloy of BiSn by aqueous two‐phase system. This strategy is a sustainable and economically viable way of recovering bismuth from secondary sources. In the aqueous two‐phase system (ATPS), biodegradable and non‐toxic constituents dispersed in water (major constituent) are used. For monitoring the extraction, bismuth was determined in the upper phase of the aqueous two‐phase system, which is rich in L35 copolymer, that causes attenuation of the electrochemical signal (anodic peak current). The electrode and operational parameters of the square‐wave anodic stripping voltammetry (SWASV) were evaluated according to the deposition and stripping processes of the bismuth on the surface of the carbon paste electrode (CPE). It was observed a similarity between the electrochemical response of the bismuth extracted by ATPS and with the standard solution of bismuth. The proposed method shows a linear range of 1.29–8.94 μmol L−1, limit of detection (1.07 μmol L−1) and limit of quantification (3.57 μmol L−1) and good precision (RSD%=2.27 %). This method was validated by comparing the results with Flame Atomic Absorption Spectroscopy (FAAS), using statistical tests to verify precision and accuracy. In conclusion, using a voltammetric sensor to monitor the concentration of bismuth extracted by ATPS proved to be an efficient method, in agreement with the concentrations of the referenced method.

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Approaches to Nanoparticle Labeling: A Review of Fluorescent, Radiological, and Metallic Techniques

Chen

et al. 2023

Environ. Health

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Nanomaterials are widely used in commercial products, resulting in the release of nanoscale particles into the environment. This raises concerns about their potential exposure risks in complex biological matrices. Most attempts use engineered nanomaterials (ENMs) to mimic the biological behavior of nanoparticles in the environment, and labeling of ENMs by sensors is a commonly used approach for sensitive detection and tracking of ENMs in organisms. However, due to the distinct physicochemical properties of nanoparticles, different labeling approaches have been developed, each with varying applicability. In this Review, we summarize the three main types of labeling methods used for nanoparticles: fluorescent, radiological, and metallic labeling. We discuss their labeling mechanisms, efficiency, stability, target nanoparticles, and applicability in different organism models. Finally, we propose a labeling scheme for specific nanoparticles. Overall, this Review provides a comprehensive overview of the advances in nanoparticle labeling techniques and their potential applications in environmental and health studies.

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<p>A Chelate-Free Nano-Platform for Incorporation of Diagnostic and Therapeutic Isotopes</p>

Cited by 9 publications

References 77 publications

<p>A Radio-Nano-Platform for T1/T2 Dual-Mode PET-MR Imaging</p>

<p>A Radio-Nano-Platform for T1/T2 Dual-Mode PET-MR Imaging</p>

Square‐wave Anodic Stripping Voltammetric Method for Novelty Detection of Bismuth Extracted by Aqueous Two‐phase Systems

Approaches to Nanoparticle Labeling: A Review of Fluorescent, Radiological, and Metallic Techniques

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