<p>808 nm Near-Infrared Light-Excited UCNPs@mSiO₂-Ce6-GPC3 Nanocomposites For Photodynamic Therapy In Liver Cancer</p>

Abstract

BackgroundIt is important to explore effective treatment for liver cancer. Photodynamic therapy (PDT) is a novel technique to treat liver cancer, but its clinical application is obstructed by limited depth of visible light penetration into tissue. The near-infrared (NIR) photosensitizer is a potential solution to the limitations of PDT for deep tumor tissue treatment.PurposeWe aimed to investigate 808 nm NIR light-excited UCNPs@mSiO2-Ce6-GPC3 nanocomposites for PDT in liver cancer.MethodsIn our study, 808 nm NIR light-excited upconversion nanoparticles (UCNPs) were simultaneously loaded with the photosensitizer chlorin e6 (Ce6) and the antibody glypican-3 (GPC3), which is overexpressed in hepatocellular carcinoma cells. The multitasking UCNPs@mSiO2-Ce6-GPC3 nanoparticles under 808 nm laser irradiation with enhanced depth of penetration would enable the effective targeting of PDT.ResultsWe found that the UCNPs@mSiO2-Ce6-GPC3 nanoparticles had good biocompatibility, low toxicity, excellent cell imaging in HepG2 cancer cells and high anti-tumor effect in vitro and in vivo.ConclusionWe believe that the utilization of 808 nm NIR excited UCNPs@mSiO2-Ce6-GPC3 nanoparticles for PDT is a safe and potential therapeutic option for liver cancer.

Facile Synthesis of NaYF4:Yb Up-Conversion Nanoparticles Modified with Photosensitizer and Targeting Antibody for In Vitro Photodynamic Therapy of Hepatocellular Carcinoma

“…There are a few reports on the conjugation of specific antibodies with UCNPs; however, most of them were coated with mesoporous silica. Surface modification of UCNPs with both specific antibodies and photosensitizer by using PEI as a bridge has been rarely reported [ 29 , 30 ].…”

Glypican-3: A Novel and Promising Target for the Treatment of Hepatocellular Carcinoma

“…The use of the near-infrared (NIR) photosensitizer may solve these limitations ( 100 ). For example, UCNPs@mSiO2-Ce6-GPC3 nanoparticles are biocompatible, have low toxicity, and produce good cell imaging and antitumor results ( 101 ). A novel multi-functional nanostructure, galactose (GAL)- golden nanorods (GNR)-siRNA of GPC3(siGPC3) was found to produce both silencing of the GPC3 gene and photothermal action, and may be useful as a synergistic treatment for cancer ( 102 ).…”

“…At present, assembling suicide genes, synthetic notch receptors, on-switch CAR, bispecific CAR-T cells can help prevent healthy cells from CAR-T attacking ( 118 ). At present, no obvious toxicity has been reported in GPC3 related gene therapy, immunotoxin and photodynamic therapy ( 78 , 93 , 101 ).…”

Photodynamic Nanophotosensitizers: Promising Materials for Tumor Theranostics

“…78 Also, various types of drugs are coupled with the UCNP; for example, NaGdF4:Yb with Er@NaGdF4:Yb and NaNdF4:Yb(UCNPs) loaded with NPS chlorin Ce6 drug and anti-GPC3.UCNPs@mSiO 2 -Ce6-GPC3 on irradiation at 808 nm over hepatoma lung tumor cell (Hep G2) overexpressed GPC3, causing apoptosis and restraining the survival rate through ROS production. 92 Further, UCSs are loaded with the drug tirapazamine (TPZ) to produce UCS/TPZ NPS to achieve the combinational effect of photodynamic theranostics and chemotherapy. Around 542 nm, UCS/TPZ activates the hypoxia site to apoptotic cycle in a distant tumor site of CT-26 tumor cells.…”