&lt;I&gt;Wolbachia&lt;/I&gt; Bacterial Infections Linked to Mitochondrial DNA Reproductive Isolation Among Populations of Northern Corn Rootworm (Coleoptera: Chrysomelidae)

Roehrdanz, Richard L.; Levine, Eli

doi:10.1603/0013-8746(2007)100[522:wbiltm]2.0.co;2

Cited by 24 publications

(31 citation statements)

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“…The origins of specific samples used in this work are summarized in Table 1. Details of the com rootworm collection sites, their Wolbachia infection status, and a map of the collection sites are as published previously (Roehrdanz and Levine 2007). In the restriction fragment-length polymorphism analysis of Wolbachia fisZ fragments, 223 out of 226 (98.7%) beetles collected west ofthe mtDNA boundary were labeled as northern com rootworm Wolbachia NCR type 2.…”

Section: Methodsmentioning

confidence: 99%

“…Insect and DNA samples are preserved at the USDA Biosciences Research Laboratory in Fargo, ND. (1) " Haplotype details eontained in Roehrdanz and Levine (2007). '' Four digit numbers indicate the pattern of the four hypervariable regions; numbers in parentheses are the number of clones with that pattern combination.…”

Section: Methodsmentioning

confidence: 99%

“…Total DNA from adult rootworms was prepared as described earlier (Roehrdanz et al 2003). The mitochondrial DNA haplotype assignments and the presence or absence of Wolbachia infections have also been described previously (Roehrdanz and Levine 2007). A portion ofthe Wolbachia surface protein gene, wsp, ^600 bp long, was amplified with primers wsp-81 F (5'-TGG TCC AAT AAG TGA TGA AGA AAG TAG CTA) and WSP-691R (5'-AAA AAT TAA AGG GTA GTG GAG GTT GTG GAG) (Zhou et al 1998).…”

Section: Methodsmentioning

confidence: 99%

“…A hnk between Wolbachia infections and mitochondrial genotypes has been described in northern com rootworm. As a result, northern com rootworm in eastern and central North America exhibits at least three distinct populations with respect to Wolbachia infection: uninfected in the westem part of its range; infected with one strain of Wolbachia in central and eastern Illinois; infected with a different strain from far eastern Illinois to the east (Roehrdanz and Levine 2007). In that study the presence of Wolbachia was determined by polymerase chain reaction (PCR) amplification of fragments of the Wolbachia fisZ gene.…”

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confidence: 99%

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Wolbachia wsp Gene Clones Detect the Distribution of Wolbachia Variants and wsp Hypervariable Regions Among Individuals of a Multistrain Infected Population of Diabrotica barberi (Coleoptera: Chrysomelidae)

Roehrdanz

Wichmann

2013

Annals of the Entomological Society of America

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The northern corn rootworm {Diabrotica barberi Smith & Lawrence) in eastem and central North America exhibits at least three distinct populations with respect to Wolbachia infection: uninfected, singly infected, and multiply infected. The infected states are associated with different mtDNA haplotypes and reduced mtDNA variability. The previous results demonstrated that the major mtDNA clades of D. barberi were infected with different variants of Wolbachia; however, the total amount of Wolbachia diversity was substantially underestimated. Here we report sequences of the Wolbachia wsp surface protein gene indicating that multiple infections are present. Analysis ofthe wsp sequences establishes the existence at least five distinct wsp variants. The recovery frequency of specific wsp hypervariable regions (HVR) obtained from several individuals was unequal. The most common was obtained 33 times, the least common only once. One of the Wolbachia variants was present in both singly infected and multiply infected individuals. A possible explanation for the frequency differences are that the frequency of each variant is not the same within each insect. An alternative possibility is that not all individuals are infected with all five variants and that different animals contain different combinations ofthe variants. The second scenario suggests that some variants are rare in the population.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Wolbachia wsp Gene Clones Detect the Distribution of Wolbachia Variants and wsp Hypervariable Regions Among Individuals of a Multistrain Infected Population of Diabrotica barberi (Coleoptera: Chrysomelidae)

Roehrdanz

Wichmann

2013

Annals of the Entomological Society of America

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“…Ver- tically inherited intracellular bacteria, including Wolbachia, can cause cytoplasmic incompatibility, which can lead to reproductive isolation (Werren et al 2008). Wolbachia has been found in other species of the virgifera group of Diabrotica (Giordano and Jackson 1999, Clark et al 2001b, Segura-Leó n 2004, and multiple strains of Wolbachia have been documented in the eastern part of the range of D. barberi (Roehrdanz et al 2003, Roehrdanz andLevine 2007). However, to date, Wolbachia has not been found in either D. longicornis or western populations of D. barberi (Clark et al 2001b, Roehrdanz et al 2003, but this may be due to limited sampling.…”

Section: Discussionmentioning

confidence: 99%

Fitness of Diabrotica barberi, Diabrotica longicornis, and Their Hybrids (Coleoptera: Chrysomelidae)

Campbell

Meinke

2010

Annals of the Entomological Society of America

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Diabrotica barberi Smith & Lawrence, the northern corn rootworm, and Diabrotica longicornis (Say) (Coleoptera: Chrysomelidae), are currently recognized as closely related chrysomelid species. Hybridization has been proposed to occur between them, although the viability of hybrids has never been tested. The objective of this study was to assess life-history parameters of D. barberi, D. longicornis, and their hybrids under laboratory conditions to examine the potential for field hybridization. D. barberi and D. longicornis were collected in allopatry and were used to create lab colonies. Parental species were crossed to obtain F1 hybrids, F2 hybrids, and backcrosses to either parental species. Various life-history traits, which may contribute to overall fitness, were measured, and population growth rates were calculated for all crosses. D. barberi had greater reproductive potential than D. longicornis, but D. longicornis individuals lived longer than D. barberi individuals. In other traits, the two parental species were similar. The fitness of hybrids of a D. longicornis female and D. barberi male, as estimated by reproduction, survival, developmental time, longevity, and head capsule width, was similar to that of the parental species. Hybrids of a D. barberi female and D. longicornis male demonstrated consistently poor egg viability, low survival, and shortened adult life span. The cause of the hybrid unidirectionality is unknown, but these data collectively suggest that hybrids of a D. longicornis female and D. barberi male and F2 backcrosses with parentals could potentially be as viable as either parental species and contribute to population growth under field conditions.

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Assessment of the role of Wolbachia in mtDNA paraphyly and the evolution of unisexuality in Calligrapha (Coleoptera: Chrysomelidae)

Gómez‐Zurita

2019

Ecology and Evolution

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Calligrapha is a New World leaf beetle genus that includes several unisexual species in northeastern North America. Each unisexual species had an independent hybrid origin involving different combinations of bisexual species. However, surprisingly, they all cluster in a single mtDNA clade and with some individuals of their parental species, which are in turn deeply polyphyletic for mtDNA. This pattern is suggestive of a selective sweep which, together with mtDNA taxonomic incongruence and occurrence of unisexuality in Calligrapha, led to hypothesize that Wolbachia might be responsible. I tested this hypothesis studying the correlation between diversity of Wolbachia and well‐established mtDNA lineages in >500 specimens of two bisexual species of Calligrapha and their derived unisexual species. Wolbachia appears highly prevalent (83.4%), and fifteen new supergroup‐A strains of the bacteria are characterized, belonging to three main classes: wCallA, occupying the whole species ranges, and wCallB and wCallC, narrowly parapatric, infecting beetles with highly divergent mtDNAs where they coexist. Most beetles (71.6%) carried double infections of wCallA with another sequence class. Bayesian inference of ancestral character states and association tests between bacterial diversity and the mtDNA genealogy show that each mtDNA lineage of Calligrapha has specific types of infection. Moreover, shifts can be explained by horizontal or vertical transfer from local populations to an expanding lineage and cytoplasmic incompatibility between wCallB and wCallC types, suggesting that the symbionts hitchhike with the host and are not responsible for selective mtDNA sweeps. Lack of evidence for sweeps and the fact that individuals in the unisexual clade are uninfected or infected by the widespread wCallA type indicate that Wolbachia does not induce unisexuality in Calligrapha, although they may manipulate host reproduction through cytoplasmic incompatibility.

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<I>Wolbachia</I> Bacterial Infections Linked to Mitochondrial DNA Reproductive Isolation Among Populations of Northern Corn Rootworm (Coleoptera: Chrysomelidae)

Cited by 24 publications

References 1 publication

Wolbachia wsp Gene Clones Detect the Distribution of Wolbachia Variants and wsp Hypervariable Regions Among Individuals of a Multistrain Infected Population of Diabrotica barberi (Coleoptera: Chrysomelidae)

Wolbachia wsp Gene Clones Detect the Distribution of Wolbachia Variants and wsp Hypervariable Regions Among Individuals of a Multistrain Infected Population of Diabrotica barberi (Coleoptera: Chrysomelidae)

Fitness of Diabrotica barberi, Diabrotica longicornis, and Their Hybrids (Coleoptera: Chrysomelidae)

Assessment of the role of Wolbachia in mtDNA paraphyly and the evolution of unisexuality in Calligrapha (Coleoptera: Chrysomelidae)

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