&lt;i&gt;Trans&lt;/i&gt; Fatty Acids in Maternal Diet May Impair Lipid Biosynthesis in Mammary Gland of Lactating Rats

Assumpção, Roberto Pereira; Santos, Flávia Duarte dos; Setta, Cecília L; Barreto, Giselle Freire; Matta, I.E.A.; Estadella, Débora; Azeredo, Vilma Blondet de; Carmo, Maria G. Tavares do

doi:10.1159/000065403

Cited by 11 publications

(8 citation statements)

References 25 publications

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“…However, the high occurrence of trans-fatty acids in goat milk (Table 5 and Chilliard et al, 2003b, Ferlay et al, 2003 could have been expected to inhibit ACC and FAS. Indeed, in bovine mammary cell cultures, Jayan and Herbein (2000) showed that trans-11 C18:1 reduced activities of ACC and FAS compared with oleic acid, and recently in the mammary gland of rats fed a diet containing a mixture of trans-isomers, an impairment of lipid biosynthesis was observed (Assumpcao et al, 2002). In cows, Piperova et al (2000), using a milk fat-depressing diet (25:70% forage/concentrate, supplemented with 5% soybean oil), observed a reduction in ACC mRNA abundance and activity, and in FAS activity in mammary tissue, associated with a dramatic decrease of 59% in C10-C16 FA secretion (g/ d).…”

Section: Mammary Metabolismmentioning

confidence: 99%

Mammary Lipid Metabolism and Milk Fatty Acid Secretion in Alpine Goats Fed Vegetable Lipids

Bernard

Rouel

Leroux

et al. 2005

Journal of Dairy Science

166

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Fourteen Alpine goats at midlactation were fed a diet of hay and concentrate (55:45), without (control) or with formaldehyde-treated linseed (FLS) or oleic sunflower oil (OSO) at 11.2 or 3.5% of dry matter intake, respectively, in a 3 x 3 Latin Square design with three 3-wk periods. Milk yield was lower in goats fed FLS than control or OSO (2.13 vs. 2.32 kg/d). Milk fat content was higher with FLS or OSO than control (40.8 vs. 33.8 g/kg). Formaldehyde-treated linseed and OSO caused a significant decrease (23 and 18%, respectively) of C10 to C17 fatty acids secretion compared with control. The secretion of cis-9 C18:1 and cis-9, trans-11 C18:2 were increased 1.44- and 1.54-fold for FLS and 1.78- and 1.36-fold for OSO, compared with control. The C18:3 (n-3) secretion was increased 2.61-fold with FLS compared with control. Milk cis-9 C14:1/C14:0, cis-9 C16:1/C16:0, and cis-9 C18:1/C18:0 ratios decreased with the supplemented diets compared with control. Mammary stearoyl-CoA desaturase mRNA and activity were decreased by the lipid supplements, whereas no significant change was observed for acetyl-CoA carboxylase and fatty acid synthase. The activities of glucose-6-phosphate dehydrogenase, malic enzyme, and glycerol-3-phosphate dehydrogenase were not affected by the lipid supplements. Mammary lipoprotein lipase mRNA increased with OSO, whereas lipoprotein lipase activity tended to decrease with FLS compared with control. Milk lipoprotein lipase activity sharply decreased with lipid supplement (by 59 and 71%, for FLS and OSO, respectively). The changes in milk fatty acid profile due to FLS and OSO supplements were partly related to changes in the levels of mammary enzyme activities or mRNA.

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Section: Mammary Metabolismmentioning

confidence: 99%

Mammary Lipid Metabolism and Milk Fatty Acid Secretion in Alpine Goats Fed Vegetable Lipids

Bernard

Rouel

Leroux

et al. 2005

Journal of Dairy Science

166

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“…Recent experimental reports from our laboratory indicate that maternal intake of TFA during exclusive lactation resulted in the TFA incorporation into the mammary gland and also into the milk, in a dose‐dependent way, in part mediated by a higher activity of the LPL enzyme in mammary tissues 11 . Moreover, the intake of TFA during lactation results in important metabolic modifications in the lactating mother, decreasing of de novo synthesis of fatty acids in the mammary gland 12 …”

mentioning

confidence: 95%

Trans fatty acids from milk of Brazilian mothers of premature infants

Tinoco¹,

Sichieri

Setta

et al. 2007

J Paediatrics Child Health

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“…Nevertheless, only recently was a central role proposed for specific trans-FA as potent inhibitors of mammary lipid synthesis, from studies with specific dietary conditions inducing a dramatic MFD (Bauman & Griinari, 2003). In the same way, an impairment of mammary lipid synthesis has been observed in rats fed a diet containing a mixture of trans-isomers (Assumpcao et al, 2002).…”

Section: In Vivo Results In Nutritional Studiesmentioning

confidence: 99%

Expression and Nutritional Regulation of Lipogenic Genes in the Ruminant Lactating Mammary Gland

Bernard

Leroux

Chilliard

Advances in Experimental Medicine and Biology

201

224

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The effect of nutrition on milk fat yield and composition has largely been investigated in cows and goats, with some differences for fatty acid (FA) composition responses and marked species differences in milk fat yield response. Recently, the characterization of lipogenic genes in ruminant species allowed in vivo studies focused on the effect of nutrition on mammary expression of these genes, in cows (mainly fed milk fat-depressing diets) and goats (fed lipid-supplemented diets). These few studies demonstrated some similarities in the regulation of gene expression between the two species, although the responses were not always in agreement with milk FA secretion responses. A central role for trans-10 C18:1 and trans-10, cis-12 CLA as regulators of milk fat synthesis has been proposed. However, trans-10 C18:1 does not directly control milk fat synthesis in cows, despite the fact that it largely responds to dietary factors, with its concentration being negatively correlated with milk fat yield response in cows and, to a lesser extent, in goats. Milk trans-10, cis-12 CLA is often correlated with milk fat depression in cows but not in goats and, when postruminally infused, acts as an inhibitor of the expression of key lipogenic genes in cows. Recent evidence has also proven the inhibitory effect of the trans-9, cis-11 CLA isomer. The molecular mechanisms by which nutrients regulate lipogenic gene expression have yet to be well identified, but a central role for SREBP-1 has been outlined as mediator of FA effects, whereas the roles of PPARs and STAT5 need to be determined. It is expected that the development of in vitro functional systems for lipid synthesis and secretion will allow future progress toward (1) the identification of the inhibitors and activators of fat synthesis, (2) the knowledge of cellular mechanisms, and (3) the understanding of differences between ruminant species.

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<i>Trans</i> Fatty Acids in Maternal Diet May Impair Lipid Biosynthesis in Mammary Gland of Lactating Rats

Cited by 11 publications

References 25 publications

Mammary Lipid Metabolism and Milk Fatty Acid Secretion in Alpine Goats Fed Vegetable Lipids

Mammary Lipid Metabolism and Milk Fatty Acid Secretion in Alpine Goats Fed Vegetable Lipids

Trans fatty acids from milk of Brazilian mothers of premature infants

Expression and Nutritional Regulation of Lipogenic Genes in the Ruminant Lactating Mammary Gland

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