Abstract:Background. Among the weeds of the Solanaceae family, a potential viroid infection reservoir, black nightshade (Solanum nigrum L.), is ubiquitous in Russia.Materials and methods. Molecular diagnostics of the viroid was performed using RT-PCR with the P3/P4 primers specific for PSTVd and 6Pospi F/R specific for the Pospiviroidae family of viroids. The amplification products were cloned and sequenced, and the nucleotide sequences were deposited in the GenBank database.Results. RT-PCR showed that 80–100% of plant… Show more
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