&lt;i&gt;Salmonella&lt;/i&gt; Serovars Isolated from Retail Meats in Tokyo, Japan and Their Antimicrobial Susceptibility

The Journal of Veterinary Medical Science

Ikeda

Yonemitsu

et al. 2023

Understanding the antimicrobial resistance of Campylobacter jejuni and Salmonella spp. isolated from patients with enteritis will aid in therapeutic decisionmaking. This study aimed to characterize C. jejuni and Salmonella spp. isolates from patients with enteritis. For C. jejuni, the resistance rates against ampicillin, tetracycline, and ciprofloxacin were 17.2%, 23.8%, and 46.4%, respectively. All the C. jejuni isolates were susceptible to erythromycin, which is recommended as a first-choice antimicrobial if Campylobacter enteritis is strongly suspected. C. jejuni was classified into 64 sequence types (STs), and the five major STs were ST22, ST354, ST21, ST918, and ST50. The ciprofloxacin-resistance rate of ST22 was 85.7%. For Salmonella, the resistance rates against ampicillin, cefotaxime, streptomycin, kanamycin, tetracycline, and nalidixic acid were 14.7%, 2.0%, 57.8%, 10.8%, 16.7%, and 11.8%, respectively.All the Salmonella spp. isolates were susceptible to ciprofloxacin. Therefore, fluoroquinolones are the recommended antimicrobials against Salmonella enteritis. S.Thompson, S. Enteritidis, and S. Schwarzengrund were the three most prevalent serotypes. The two cefotaxime-resistant isolates were serotyped as S. Typhimurium and were found to harbor blaCMY-2. The results of this study would help select antimicrobials for treating patients with Campylobacter and Salmonella enteritis.

Section: Discussionmentioning

confidence: 99%

Antimicrobial resistance profiles of Campylobacter jejuni and Salmonella spp. isolated from enteritis patients in Japan

The Journal of Veterinary Medical Science

Ikeda

Yonemitsu

et al. 2023

“…Shimojima et al . [ 36 ] investigated the presence of Salmonella in 1,378 local meat products (576 chicken, 406 pork, and 396 beef products) and 993 imported meat products (281 chicken, 393 pork, and 319 beef products) between 2009 and 2017; however, neither S . Corvallis nor S .…”

Section: Discussionmentioning

confidence: 99%

Prevalence and antimicrobial resistance of Campylobacter and Salmonella in layer flocks in Honshu, Japan

The Journal of Veterinary Medical Science

Yonemitsu

Uema

et al. 2022

Campylobacter and non-typhoidal Salmonella are the major causes of bacterial gastrointestinal infections in humans. Although antimicrobial therapy is typically not recommended in many cases of these infections, it may be life-saving in patients with severe symptoms. Since chicken eggs and meat derived from layers are destined for human consumption, we investigated the prevalence and antimicrobial resistance of these two bacterial genera in 82 layer flocks at chicken processing plants in Honshu, Japan. Campylobacter was isolated from 77 flocks (93.9%). Resistance to ampicillin, tetracycline, and ciprofloxacin was documented in 42.3 (30/71), 16.9 (12/71), and 14.1% (10/71) of Campylobacter jejuni, respectively. Multilocus-sequence typing identified ST4389 and ST5262 as the most frequent C. jejuni sequence types. In C. coli, resistance to ampicillin, tetracycline, and ciprofloxacin was found in 20.0 (7/35), 20.0 (7/35), and 25.7% (9/35), respectively. The most frequent sequence type in C. coli was ST8292. Erythromycin resistance was not observed among Campylobacter species. Salmonella was isolated from 14 flocks (17.1%). The two most frequent serovars were Salmonella Corvallis and S. Braenderup. Neither S. Enteritidis nor S. Infantis were isolated.Streptomycin resistance was observed in six isolates (26.1%), and all isolates were susceptible to cefotaxime and ciprofloxacin. Thus, chicken eggs and meat derived from layers are possible sources of these bacterial infections in humans. The antimicrobial susceptibility of these isolates was maintained, reflecting restrictions on the use of antimicrobial agents on layers.

“…Infantis isolated from human stool samples in the present study may be attributed to strains originating from Japanese broilers and laying hen flocks. Shimojima et al [ 13 ] recently reported that Salmonella prevalence was greater in local chicken products (57.9%) than in imported products (8.5%). According to the Ministry of Agriculture, Forestry and Fisheries of Japan [ 14 ], local chicken and chicken egg products accounted for approximately 65% of total chicken meat distribution and 96% of total chicken egg distribution, respectively, in Japan in FY 2018.…”

Section: Discussionmentioning

confidence: 99%

“…Heidelberg are prevalent in broiler flocks and chicken products, some of which show TGC resistance [ 17 , 18 , 19 , 20 , 21 ]. Shimojima et al [ 13 ] reported the cefotaxime resistance rate in Salmonella isolated between 2009 and 2017 was lower in local chicken products (1.9%) than in imported chicken products (28.0%); additionally, seven S . Heidelberg and three S .…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Resistance in Salmonella Isolated from Food Workers and Chicken Products in Japan

Kakizawa²,

Baba³

et al. 2021

Antibiotics

Salmonella is an enteric bacterial pathogen that causes foodborne illness in humans. Third-generation cephalosporin (TGC) resistance in Salmonella remains a global concern. Food workers may represent a reservoir of Salmonella, thus potentially contaminating food products. Therefore, we aimed to investigate the prevalence of Salmonella in food workers and characterize the isolates by serotyping and antimicrobial susceptibility testing. Salmonella was isolated from 583 (0.079%) of 740,635 stool samples collected from food workers between January and December 2018, and then serotyped into 76 Salmonella enterica serovars and 22 untypeable Salmonella strains. High rates of antimicrobial resistance were observed for streptomycin (51.1%), tetracycline (33.1%), and kanamycin (18.4%). Although isolates were susceptible to ciprofloxacin, 12 (2.1%) strains (one S. Infantis, one S. Manhattan, two S. Bareilly, two S. Blockley, two S. Heidelberg, two S. Minnesota, one S. Goldcoast, and one untypeable Salmonella strain) were resistant to the TGC cefotaxime, all of which harbored β-lactamase genes (blaCMY-2, blaCTX-M-15, blaCTX-M-55, and blaTEM-52B). Moreover, 1.3% (4/309) of Salmonella strains (three S. Infantis and one S. Manhattan strains) isolated from chicken products were resistant to cefotaxime and harbored blaCMY-2 or blaTEM-52B. Thus, food workers may acquire TGC-resistant Salmonella after the ingestion of contaminated chicken products and further contaminate food products.