&lt;i&gt;Pseudo-nitzschia&lt;/i&gt; species on the Catalan coast: characterization and contribution to the current knowledge of the distribution of this genus in the Mediterranean Sea

Quijano-Scheggia, Sonia; Garcés, Esther; Andrée, Karl B.; Diogène, Jorge; Fortuño, José-Manuel

doi:10.3989/scimar.2010.74n2395

Cited by 42 publications

(31 citation statements)

References 45 publications

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“…It has recently been reported in the NW Mediterranean, in Catalan waters (Quijano-Scheggia et al, 2005) and has been regarded as a possible case of introduction or range expansion (Quijano-Scheggia et al, 2010). Concerning Greek waters, this species was found only in the Thermaikos Gulf and was detected for the first time in November 2009.…”

Section: Pseudo-nitzschia Brasilianamentioning

confidence: 78%

Inter- and intra-specific diversity of Pseudo-nitzschia (Bacillariophyceae) in the northeastern Mediterranean

Moschandreou

Baxevanis

Katikou

et al. 2012

European Journal of Phycology

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A total of 92 cultured Pseudo-nitzschia strains, established between March 2007 and April 2010 from Greek coastal waters, were identified by using morphological and molecular (ITS2 region) characters. Twelve species were identified, among which P. brasiliana, P. subpacifica and P. hasleana were detected for the first time near Greek and East Mediterranean coasts. One P. delicatissima-like morphotype and another strain that closely resembled P. dolorosa were also found. Morphology and ITS2 phylogeny indicated that the level of diversity within the genus is higher than previously estimated (e.g. among P. delicatissima-like diatoms). Additionally, the ITS2 phylogeny revealed genetic variation within species, indicative of geographical differentiation (in P. brasiliana, P. fraudulenta, P. arenysensis, P. delicatissima and P. galaxiae). The majority of the cultured strains were also tested for domoic acid (DA) production. Strains of only three species, P. pseudodelicatissima, P. galaxiae and P. pungens var. pungens, were found capable of producing DA.

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Section: Pseudo-nitzschia Brasilianamentioning

confidence: 78%

Inter- and intra-specific diversity of Pseudo-nitzschia (Bacillariophyceae) in the northeastern Mediterranean

Moschandreou

Baxevanis

Katikou

et al. 2012

European Journal of Phycology

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“…3) are considered producers of DA (according to LUNDHOLM et al 2011). Domoic acid recorded in shellfish could be related to P. cuspidata and P. fraudulenta, as they are known to be DA producers (RHODES et al 1996, RHODES 1998, TRAINER et al 2009, QUIJANO-SCHEGGIA et al 2010. For the other two species, P. subpacifica is not known to be a DA producer while the case of P. Arenysensis requires some clarification.…”

Section: Discussionmentioning

confidence: 99%

“…Among these species, P. dolorosa has never been recognized as a DA producer while P. pseudodelicatissima (MARTIN et al 1990, PAN et al 2001, AMZIL et al 2001, P. multistriata (RHODES et al 2000, SARNO and DAHLMANN 2000, AMATO et al 2010, QUIJANO-SCHEGGIA et al 2010 and P. calliantha (BESIKTEPE et al 2008, ALVAREZ et al 2009, QUIJANO-SCHEGGIA et al 2010 are already known to be DA producers.…”

Section: Discussionmentioning

confidence: 99%

Pseudo-nitzschia Peragallo (Bacillariophyceae) diversity and domoic acid accumulation in tuberculate cockles and sweet clams in M’diq Bay, Morocco

Leblad¹,

Lundholm²,

Goux³

et al. 2013

Acta Botanica Croatica

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-The diversity of Pseudo-nitzschia (Bacillariophyceae) and accumulation of the neurotoxin domoic acid (DA) in two types of shellfish; tuberculate cockles (Acanthocardia tuberculata) and sweet clams (Challista chione) was explored in M'diq Bay, Morocco during 2007. The highest abundances of Pseudo-nitzschia were found during the period from March to October, with peaks occurring in May and September. Toxin analysis showed an accumulation of domoic acid in shellfish sampled during spring and autumn. The maximum toxin concentration was 4.9 mg DA g -1 of the whole tissue recorded in sweet clam during spring. Using transmission electron microscopy, thirteen Pseudo-nitzschia species were identified, eight of which are known as producers of domoic acid: P. multistriata, P. cuspidata, P. galaxiae, P. multiseries, P. pseudodelicatissima, P. pungens var. aveirensis, P. calliantha and P. fraudulenta. The five non-toxic species observed were P. subpacifica, P. arenysensis, P. dolorosa, P. subfraudulenta, and P. cf. caciantha.

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“…In a recent study, nine species of the genus were identified through the implementation of morphological keys, genetic data, and mating experiments (45,47). Some of these now documented locally are known to be toxigenic (7,8,44,48,51).…”

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confidence: 99%

Quantitative PCR Coupled with Melt Curve Analysis for Detection of Selected Pseudo-nitzschia spp. (Bacillariophyceae) from the Northwestern Mediterranean Sea

Andrée

Fernández-Tejedor

Elandaloussi

et al. 2011

Appl Environ Microbiol

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The frequency and intensity of Pseudo-nitzschia spp. blooms along the coast of Catalonia have been increasing over the past 20 years. As species from this genus that are documented as toxigenic have been found in local waters, with both toxic and nontoxic species cooccurring in the same bloom, there is a need to develop management tools for discriminating the difference. Currently, differentiation of toxic and nontoxic species requires time-consuming electron microscopy to distinguish taxonomic features that would allow identification as to species, and cryptic species can still remain misidentified. In this study, cells of Pseudo-nitzschia from clonal cultures isolated from seawater were characterized to their species identity using scanning electron microscopy, and subsamples of each culture were used to create an internal transcribed spacer 1 (ITS-1), 5.8S, and ITS-2 ribosomal DNA database for development of species-specific quantitative PCR (qPCR) assays. Once developed, these qPCR assays were applied to field samples collected over a 2-year period in Alfaques Bay in the northwestern Mediterranean Sea to evaluate the possibility of a comprehensive surveillance for all Pseudo-nitzschia spp. using molecular methods to supplement optical microscopy, which can discern taxonomy only to the genus level within this taxon. Total Pseudo-nitzschia cell density was determined by optical microscopy from water samples collected weekly and compared to results obtained from the sum of eight Pseudo-nitzschia species-specific qPCR assays using duplicate samples. Species-specific qPCR followed by melt curve analysis allowed differentiation of amplicons and identification of false positives, and results correlated well with the total Pseudo-nitzschia cell counts from optical microscopy.

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<i>Pseudo-nitzschia</i> species on the Catalan coast: characterization and contribution to the current knowledge of the distribution of this genus in the Mediterranean Sea

Cited by 42 publications

References 45 publications

Inter- and intra-specific diversity of Pseudo-nitzschia (Bacillariophyceae) in the northeastern Mediterranean

Inter- and intra-specific diversity of Pseudo-nitzschia (Bacillariophyceae) in the northeastern Mediterranean

Pseudo-nitzschia Peragallo (Bacillariophyceae) diversity and domoic acid accumulation in tuberculate cockles and sweet clams in M’diq Bay, Morocco

Quantitative PCR Coupled with Melt Curve Analysis for Detection of Selected Pseudo-nitzschia spp. (Bacillariophyceae) from the Northwestern Mediterranean Sea

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