&lt;i&gt;Picornaviridae&lt;/i&gt;

Melnick, Joseph L.; Agol, V.I.; Bachrach, H.L.; Brown, F.; Cooper, Penny; Fiers, W.; Gard, S.; Gear, J.H.S.; Ghendon, Y.; Kasza, L.; LaPlaca, M.; Mandel, B.; McGregor, Sandy; Mohanty, S.B.; Plummer, G.; Rueckert, Roland R.; Schaffer, Frederick L.; Tagaya, Isamu; Tyrrell, D.A.J.; Voroshilova, M.; Wenner, H.A.

doi:10.1159/000149863

Cited by 80 publications

(60 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The stability of DHV to acid pH and the protection given by high salt concentrations to thermal inactivation at 50°C are consistent with the classification of DHV as an enterovirus (Melnick et al, 1974). Most of the enteroviruses studied by Wallis et al (1965) were inactivated at 50°C when magnesium sulphate was included in the diluent but DHV shares with the type species of mammalian enteroviruses, poliovirus type 1, the property of stability at 50° C in all four salt solutions invest'gated.…”

Section: Text-fig 4 Effect Of Freezing and Thawing Dhv Following Insupporting

confidence: 75%

Temperature and pH stability of duck hepatitis virus

Davis

1987

Avian Pathology

View full text Add to dashboard Cite

SUMMARYDuck hepatitis virus was comparatively stable at temperatures below 40 to 45°C but was rapidly inactivated at higher temperatures. The virus was protected against thermal inactivation at 50°C by molar solutions of sodium chloride, sodium sulphate, magnesium chloride and magnesium sulphate and was stable at pH3 for 9 but not 48 hours. These properties are consistent with its classification as an enterovirus.

show abstract

Section: Text-fig 4 Effect Of Freezing and Thawing Dhv Following Insupporting

confidence: 75%

Temperature and pH stability of duck hepatitis virus

Davis

1987

Avian Pathology

View full text Add to dashboard Cite

show abstract

“…FCV was initially classified as a member of the Picornaviridae family because of its positive-sense RNA genome and similar structural morphology (25). It was subsequently placed into the family Caliciviridae, which was created when it became evident that caliciviruses have important differences from picornaviruses (reviewed in reference 16).…”

Section: Discussionmentioning

confidence: 99%

Isolation of Enzymatically Active Replication Complexes from Feline Calicivirus-Infected Cells

Green

Mory

Fogg

et al. 2002

J Virol

View full text Add to dashboard Cite

A membranous fraction that could synthesize viral RNA in vitro in the presence of magnesium salt, ribonucleotides, and an ATP-regenerating system was isolated from feline calicivirus (FCV)-infected cells. The enzymatically active component of this fraction was designated FCV replication complexes (RCs), by analogy to other positive-strand RNA viruses. The newly synthesized RNA was characterized by Northern blot analysis, which demonstrated the production of both full-length (8.0-kb) and subgenomic-length (2.5-kb) RNA molecules similar to those synthesized in FCV-infected cells. The identity of the viral proteins associated with the fraction was investigated. The 60-kDa VP1 major capsid protein was the most abundant viral protein detected. VP2, a minor structural protein encoded by open reading frame 3 (ORF3), was also present. Nonstructural proteins associated with the fraction included the precursor polypeptides Pro-Pol (76 kDa) and p30-VPg (43 kDa), as well as the mature nonstructural proteins p32 (derived from the N-terminal region of the ORF1 polyprotein), p30 (the putative "3A-like" protein), and p39 (the putative nucleoside triphosphatase). The isolation of enzymatically active RCs containing both viral and cellular proteins should facilitate efforts to dissect the contributions of the virus and the host to FCV RNA replication.Feline calicivirus (FCV), a member of the genus Vesivirus in the family Caliciviridae, is a major agent of respiratory disease in cats. The FCV genome is an approximately 7.7-kb singlestrand positive-sense RNA molecule that is covalently linked to a protein designated VPg (for virus protein, genome) at the 5Ј end and polyadenylated at the 3Ј end (8, 18). The genome is organized into three open reading frames (ORFs). ORF1 encodes an approximately 200-kDa polyprotein that is processed by the virus-encoded 3C-like cysteine proteinase into the mature nonstructural proteins p5.6, p32, p39 (nucleoside triphosphatase [NTPase]), p30, VPg, and Pro-Pol (36a, 38). ORF2 encodes a 73-kDa capsid precursor (preVP1) that is cleaved in trans by the same virus-encoded proteinase to yield the 14-kDa capsid leader (LC) and the approximately 60-kDa mature major capsid protein VP1 (9, 31, 37). ORF3 encodes a 12-kDa basic protein of unknown function, designated VP2, that is associated with mature virions (17, 36).RNA purified from virus particles and capped RNA transcripts derived from a full-length cDNA clone are infectious when transfected into feline kidney cells (21, 35). Two major polyadenylated positive-sense RNA molecules have been detected in FCV-infected cells (7,18,30). The 7.7-to 8-kb genomic RNA serves as a message for translation of the viral nonstructural proteins, and the ϳ2.6-kb subgenomic RNA serves as a bicistronic template for translation of structural proteins VP1 and VP2 (18,31). Several additional species of positive-and negative-sense RNA have been detected in FCVinfected cells, but their significance is not known (7,30).All of the positive-strand RNA viruses examined thus far form r...

show abstract

“…It quickly became apparent, however, that this scheme was inadequate for the classification of human enteroviruses, because viruses pathogenic in mice were isolated that were serotypically identical to known echoviruses, and the echoviruses were soon shown to be associated with a wide range of human diseases (9,11). Thereafter, new human enterovirus serotypes were simply named "enterovirus" and numbered sequentially, starting with EV68 (46,80). A total of 64 serotypes are currently recognized (34), and additional serotypes have been proposed (53,54,57).…”

mentioning

confidence: 99%

Evidence for Frequent Recombination within SpeciesHuman Enterovirus BBased on Complete Genomic Sequences of All Thirty-Seven Serotypes

Oberste

Maher

Pallansch

2004

J Virol

225

190

View full text Add to dashboard Cite

The species Human enterovirus B (HEV-B) in the family Picornaviridae consists of coxsackievirus A9; coxsackieviruses B1 to B6; echoviruses 1 to 7, 9, 11 to 21, 24 to 27, and 29 to 33; and enteroviruses 69 and 73. We have determined complete genome sequences for the remaining 22 HEV-B serotypes whose sequences were not represented in public databases and analyzed these in conjunction with previously available complete sequences in GenBank. Members of HEV-B were monophyletic relative to all other human enterovirus species in all regions of the genome except in the 5-nontranslated region (NTR), where they are known to cluster with members of HEV-A. Within HEV-B, phylogenies constructed from the structural (P1) and nonstructural regions of the genome (P2 and P3) are incongruent, suggesting that recombination had occurred. Similarity plots and bootscanning analysis across the complete genome identified multiple sites at which the phylogeny of a given strain's sequence shifted, indicating potential recombination points. These points are distributed in the 5-NTR and throughout P2 and P3, but no sites with >80% bootstrap support were identified within the capsid. Individual sequence comparisons and phylogenetic analyses suggest that members of HEV-B have recombined with one another on multiple occasions, resulting in a complex mosaic of sequences derived from multiple parental viruses in the nonstructural regions of the genome. We conclude that RNA recombination is a common mechanism for enterovirus evolution and that recombination within the nonstructural regions of the genome (P2 and P3) has been observed only among members of the same species.

show abstract

<i>Picornaviridae</i>

Cited by 80 publications

References 0 publications

Temperature and pH stability of duck hepatitis virus

Temperature and pH stability of duck hepatitis virus

Isolation of Enzymatically Active Replication Complexes from Feline Calicivirus-Infected Cells

Evidence for Frequent Recombination within SpeciesHuman Enterovirus BBased on Complete Genomic Sequences of All Thirty-Seven Serotypes

Contact Info

Product

Resources

About