<i>Lycium barbarum</i> polysaccharide enhances development of previously-cryopreserved murine two-cell embryos via restoration of mitochondrial function and down-regulated generation of reactive oxygen species

Abstract

Lycium barbarum polysaccharide (LBP) exhibits multiple pharmacological and biological effects, including displaying antioxidant and cytoprotective properties. The current study investigated the effects of LBP-supplemented culture medium on mitochondrial distribution, mitochondrial membrane potential (MMP), adenosine triphosphate (ATP) production, mitochondrial deoxyribonucleic acid (mtDNA) copy number, reactive oxygen species (ROS) accumulation, and development of previously-cryopreserved murine two-cell embryos. Results indicate that LBP enhances development of such embryos, and that potential mechanisms include: (1) mitochondrial function enhancement via altering mitochondrial distribution and increasing MMP, ATP production, mtDNA copy number, and expression of genes involved in mitochondrial biogenesis and energy metabolism (NAD-dependent deacetyltransferase sirtuin-1 (SIRT1) and phosphorylated adenosine monophosphate-activated protein kinase (pAMPK)); (2) down-regulation of ROS generation and enhanced expression of the antioxidant genes glutathione peroxidase 4 (GPX4) and superoxide dismutase 1 (SOD1), thereby increasing embryo oxidative stress tolerance; and (3) increased expression of B-cell lymphoma-2 (BCL2), a critical gene for cell survival and embryo development. These results demonstrate that LBP improves development of previously-cryopreserved murine two-cell embryos via restoration of mitochondrial function and down-regulated generation of ROS.

Lycium barbarum polysaccharides in ageing and its potential use for prevention and treatment of osteoarthritis: a systematic review

“…LBP can increase the expression levels of SOD and CAT in c. elegans. Yang L, et al [ 115 ] 2019 Cryopreserved mice embryo 50, 100, 200, 400, 800, and 1600 μg/ml Exposed to different concentrations of LBP The concentration of LBP at 1600 μg/ml is too high to delay the embryo growing. Choosing 200 μg/ml as the treatment dosage.…”

“…At this dosage LBP inhibits mitochondria clustering, reduces the level of ROS, and increases the mtDNA copy number and the expression of sirtuin-1 (SIRT1), AMPK, GPX4, SOD1 and Bcl-2 [ 115 ]. However, at a concentration of 1600 μg/ml LBP delayed the growth of murine two-cell embryos [ 115 ]. It is suggested that the dosage was critical for the anti-ageing effects of LBP.…”

Patchouli Alcohol Improves the Integrity of the Blood-Spinal Cord Barrier by Inhibiting Endoplasmic Reticulum Stress Through the Akt/CHOP/Caspase-3 Pathway Following Spinal Cord Injury

“…Mitochondrial JC-1 fluorescence was measured to determine the mitochondrial membrane potential (MMP) (Chazotte, 2011). At low mitochondrial polarization (MMP < 100 mV), JC-1 remains a monomer and emits green fluorescence in the fluorescein isothiocyanate (FITC) channel; at high mitochondrial polarization (MMP > 140 mV), JC-1 forms J-aggregates and emits red fluorescence (Yang et al, 2019a). HBMECs were cultured with JC-1 solution at 37 • C for 20 min.…”

Antioxidants Significantly Improve Qinling Giant Panda (Ailuropoda Melanoleuca Qinlingensis) Sperm Quality During Cryopreservation

“…Recently, the addition of antioxidants to the sperm freezing medium for humans, horses, beers, dogs, goats, cattle, and boars has demonstrated that sperm ability is signi cantly enhanced [5][6][7][8][9]. Multiple studies have a rmed that LBP, LJP and RSV contribute to the reduction of excessive ROS production from cryodamage [10][11][12].…”