&lt;i&gt;Juglans regia&lt;/i&gt; Hexane Extract Exerts Antitumor Effect, Apoptosis Induction and Cell Circle Arrest in Prostate Cancer Cells &lt;i&gt;In vitro&lt;/i&gt;

Li, W; Dy, Li; Hd, Wang; Zj, Zheng; Hu, Jiliang; Zz, Li

doi:10.4314/tjpr.v14i3.7

Cited by 16 publications

(8 citation statements)

References 18 publications

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“…2A and B) of the MTT assay indicated that isoimperatorin significantly inhibited the proliferation of SGC-7901 cells in a dose-and time-dependent manner. Flow cytometric analysis is a common method used to investigate whether drugs can induce the apoptosis of cancer cells (28,29). In the present study, the results of the flow cytometric analysis (Fig.…”

Section: A B C D Ementioning

confidence: 90%

Isoimperatorin induces apoptosis of the SGC-7901 human gastric cancer cell line via the mitochondria-mediated pathway

2016

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Abstract. The present study was designed to investigate the antiproliferative activity of isoimperatorin against SGC-7901 cells and to examine the possible mechanisms. The antiproliferative activity of isoimperatorin against SGC-7901 cells was evaluated using an MTT assay, and the mechanisms were investigated using flow cytometry and western blot assays, which were used to determine the apoptotic rate and expression levels of mitochondria-mediated apoptosis-associated proteins, including Survivin, myeloid leukemia cell-1 (Mcl-1), B cell lymphoma-extra large (Bcl-xl), B cell lymphoma 2 (Bcl-2), second mitochondria-derived activator of caspase (Smac), Bcl-2-associated X factor (Bax), cleaved (c)-caspase-3 and c-caspase-9 in SGC-7901 cells. Additionally, a xenograft assay was used to confirm whether isoimperatorin had an inhibitory effect on SGC-7901 cell-induced tumors in vivo. The results of the MTT assay suggested that isoimperatorin significantly inhibited the proliferation of SGC-7901 cells in a dose-and time-dependent manner, and the half maximal inhibitory concentration was 18.75 µg/ml. The results of the flow cytometric analysis indicated that, following treatment with isoimperatorin, the apoptotic rate of SGC-7901 cells was significantly increased, compared with that of cells in the control group. The results of the western blot analysis indicated that, following treatment with isoimperatorin, the expression levels of the pro-apoptotic proteins, Bax, c-caspase-3 and c-caspase-9, were significantly increased and the expression levels of the anti-apoptotic proteins, Survivin and Bcl-2, were significantly reduced, compared with the control group. No alterations in expression were found in the other apoptosis-associated proteins, including Mcl-1, Bcl-xl and Smac. The results of the xenograft assay indicated that isoimperatorin significantly inhibited the growth of SGC-7901 cell-induced tumor in vivo by increasing the expression levels of pro-apoptotic proteins (Bax, c-caspase-3 and c-caspase-9) and reducing the expression levels of anti-apoptotic proteins (Survivin and Bcl-2) without adverse effects on the increasing body weight of nude mice. In conclusion, the present study revealed that isoimperatorin may be able to induce the apoptosis of SGC-7901 cells in vitro and in vivo by regulating the expression levels of mitochondria-mediated apoptosis-associated proteins.

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Section: A B C D Ementioning

confidence: 90%

Isoimperatorin induces apoptosis of the SGC-7901 human gastric cancer cell line via the mitochondria-mediated pathway

2016

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“…The leaves extract of the J. regia showed a strong and dose-dependent anti-proliferative function against prostate cancer cells in human by inducing the apoptosis through some concomitant alterations in cell cycle phase distribution as it contains an anti-cancer agent such as ellagitannins at concentrations ranging from 100 to 200 mg/kg. 51 In addition, experiments employing human renal cancer cell lines of A-498 and 769-P and also the colon cancer cell line, Caco-2, depicted antiproliferative efficiency of walnut leaves extracts. 59 Apigenin, a flavonoid component that exists in green walnut husks, reported to be an anticancer agent without inducing apoptosis in the human peripheral blood mononuclear cell.…”

Section: Anticancer Activitymentioning

confidence: 99%

“…It also has a high potential of anti-atherogenic and a remarkable osteoblastic activity. 50,51 Walnut green husks have been employed in traditional Arabic Palestinian herbal medicine to treat several human diseases such as skin diseases caused by syphilis and fungi, wart, stomachache, anemia, and they were reported to have antihelmintic, astringent, and nerve tonic properties. 52 The bark, branches and husk of the green walnut have been used as a traditional medicine for the treatment of gastric, lung and liver cancer for a long time in China, Korea and the northeastern region of Mexico.…”

Section: Walnut In Folk Medicinementioning

confidence: 99%

Persian Walnut Composition and its Importance in Human Health

Ebrahimi¹,

Jamei²,

Nojoomi³

et al. 2017

Int J Enteric Pathog

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Persian walnut (Juglans regia L.) is a nutritionally rich and also popular nut which is largely consumed throughout the world. In addition to dry fruits (nuts), green walnuts, kernels, shells, green walnut husks (epicarp), barks and leaves have been applied in both cosmetic and pharmaceutical industries. Since ancient times, it has been used in folk medicine for treatment of several diseases and disorders such as diabetes, cancer, skin disease and infections, rheumatoid arthritis. In recent decades, many experimental researches have been done on the composition and characteristics of different parts of Persian walnut tree. These researches showed that walnut has many nutritious components such as phenolic component, unsaturated fatty acids and minerals essential for human health. Using walnut in folk medicine and identification of beneficial components of this fruit prompted many scientists to investigate properties that have traditionally been attributed to this fruit and the researches done in this area showed that walnut has effective antioxidant, antimicrobial, antitumor and anti-inflammatory properties that make it a promising drug for associated diseases.

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“…[ 80 ] Hexanic extract of walnut leaves is a strong inhibitor against prostate cancer cells, which could be considered as a supplementary potential against this disease. [ 81 ] Researchers showed that juglone found in different parts of the walnut plant inhibits the growth of human sickle cells, dose-dependently. [ 82 ] The chemical analysis of the composition of alcoholic extract of walnut leaves showed that its main ingredient called juglone, is associated with the insecticidal activity.…”

Section: Therapeutic Treatmentmentioning

confidence: 99%

A review study on phytochemistry and pharmacology applications of Juglans Regia plant

et al. 2017

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In recent years, the use of medicinal plants increased considerably; so that today, the use of traditional medicine, as well as medicinal plants is necessary for the aim of producing more effective drugs with fewer side effects and determining the effective doses. With the scientific name of Juglans regia, walnut plant is a medicinal plant with different properties that is considered less, despite having great therapeutic potential in the traditional medicine. The aim of this study was to review the dispersal of walnut plants, the chemical compounds, and therapeutic effects of walnuts on antioxidant activity, antidiabetic, hypolipidemic, antimicrobial, and antihypertensive activities, as well as liver protection. Data of this review study have been collected from the books and scientific articles published in databases such as Science Direct, Web of Science, Scopus, PubMed, and Scientific Information Database. While this plant having high antioxidant capabilities, walnuts are composed of many chemical compounds such as ascorbic acid, flavonoids, quercetin, and caffeic acid. Experimental studies have shown that walnuts reduced blood glucose and lipids and also decreased blood pressure. They have antioxidant, antidiabetic, antimicrobial, and liver-protective properties. The use of walnuts in traditional medicine and review of experimental studies demonstrated the presence of multiple, effective, and useful compounds which may provide the opportunity for the production of lipid-lowering, antidiabetes, and liver protective drugs. Due to the effects of walnuts on improving the complications of various diseases, the need for doing comprehensive clinical trials for the use of walnuts in the treatment of diseases is necessary.

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<i>Juglans regia</i> Hexane Extract Exerts Antitumor Effect, Apoptosis Induction and Cell Circle Arrest in Prostate Cancer Cells <i>In vitro</i>

Cited by 16 publications

References 18 publications

Isoimperatorin induces apoptosis of the SGC-7901 human gastric cancer cell line via the mitochondria-mediated pathway

Isoimperatorin induces apoptosis of the SGC-7901 human gastric cancer cell line via the mitochondria-mediated pathway

Persian Walnut Composition and its Importance in Human Health

A review study on phytochemistry and pharmacology applications of Juglans Regia plant

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