&lt;I&gt;In Vivo&lt;/I&gt; Imaging of Brain Cancer Using Epidermal Growth Factor Single Domain Antibody Bioconjugated to Near-Infrared Quantum Dots

Fatehi, Daryoush; Baral, Toya Nath; Abulrob, Abedelnasser

doi:10.1166/jnn.2014.9076

Cited by 39 publications

(28 citation statements)

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“…In vivo analysis of xenotransplanted mice showed that EG2-Cys-Qd800 accumulated in grafted GBM mass, as shown by fluorescence microscopy of murine brain sections, proving to be aminimally invasive diagnostic tool to assess level of tumor aggressiveness and resistance [98].…”

Section: Np Formulations Effects Referencesmentioning

confidence: 98%

“…Efficient targeting of U87-EGFRvIII cells in vitro; in vivo accumulation of EG2-Cys-Qd800 in xenotransplanted GBM mass [98] Fluorescent photostable quantum dots (QDs) conjugated to EGFR specific monoclonal antibodies (EGFR-Ab-QDs)…”

Section: Np Formulations Effects Referencesmentioning

confidence: 99%

“…Intra-tumor accumulation of Lf-MDCs in vivo; suppression of cancer growth that is more efficient than either doxorubicin or Cur delivered alone [105] Poly(ethylene glycol)-co-poly(trimethylene carbonate) NPs, modified with 2-deoxy-d-glucose and loaded with paclitaxel (dGlu-NP/PTX) Enhanced BBB penetration via GLUT-mediated transcytosis, improved drug accumulation in glioma tumors via GLUT-mediated endocytosis; higher anti-GBM efficacy than free taxol and NP/PTX [106] Iron oxide and dextran NPs, conjugated with non-radioactive dGlu Intra-tumor accumulation in rat and murine glioma models upon tail vein injection [107] Magnetic NPs loaded with both Cur and TMZ Induction of cell death in T-98G cells cultured as either 2D monolayers or 3D tumor spheroids (higher than with compounds administered alone) [108] CED convection-enhanced delivery, EGFR epidermal growth factor receptor, TMZ temozolomide, NPs nanoparticles, GBM glioblastoma multiforme, IL, BBB blood brain barrier, GLUT glucose transporter, CSCs cancer stem cells did unconjugated Qd800 or Qd800 conjugated to other antibody domains [98]. In vivo analysis of xenotransplanted mice showed that EG2-Cys-Qd800 accumulated in grafted GBM mass, as shown by fluorescence microscopy of murine brain sections, proving to be aminimally invasive diagnostic tool to assess level of tumor aggressiveness and resistance [98].…”

Section: Np Formulations Effects Referencesmentioning

confidence: 99%

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Targeting Glioblastoma with the Use of Phytocompounds and Nanoparticles

et al. 2015

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Glioblastoma multiforme (GBM) are extremely lethal and still poorly treated primary brain tumors, characterized by the presence of highly tumorigenic cancer stem cell (CSC) subpopulations, considered responsible for tumor relapse. In order to successfully eradicate GBM growth and recurrence, new anti-cancer strategies selectively targeting CSCs should be designed. CSCs might be eradicated by targeting some of their cell surface markers and transporters, inducing their differentiation, impacting their hyper-glycolytic metabolism, inhibiting CSC-related signaling pathways and/or by targeting their microenvironmental niche. In this regard, phytocompounds such as curcumin, isothiocyanates, resveratrol and epigallocatechin-3-gallate have been shown to prevent or reverse cancer-related epigenetic dysfunctions, reducing tumorigenesis, preventing metastasis and/or increasing chemotherapy and radiotherapy efficacy. However, the actual bioavailability and metabolic processing of phytocompounds is generally unknown, and the presence of the blood brain barrier often represents a limitation to glioma treatments. Nowadays, nanoparticles (NPs) can be loaded with therapeutic compounds such as phytochemicals, improving their bioavailability and their targeted delivery within the GBM tumor bulk. Moreover, NPs can be designed to increase their tropism and specificity toward CSCs by conjugating their surface with antibodies specific for CSC antigens, with ligands or with glucose analogues. Here we discuss the use of phytochemicals as anti-glioma agents and the applicability of phytochemical-loaded NPs as drug delivery systems to target GBM. Additionally, we provide some examples on how NPs can be specifically formulated to improve CSC targeting.

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Section: Np Formulations Effects Referencesmentioning

confidence: 98%

Section: Np Formulations Effects Referencesmentioning

confidence: 99%

Section: Np Formulations Effects Referencesmentioning

confidence: 99%

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Targeting Glioblastoma with the Use of Phytocompounds and Nanoparticles

et al. 2015

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“…Aptamers have been coupled to probes emitting fluorescence in the near infrared region, such as to the near-infrared IRD800CW reporter for in vivo imaging of CD-30-positive lymphoma cells (53). As near-infrared quantum-dots could be used for in vivo imaging of glioblastoma tissue (54), such approach should be also feasible for aptamerbased fluorescence imaging in vivo. Aptamers shall also gain importance in pathology analysis of tumor samples, i.e., analysis in intact brain tissue (55).…”

Section: Flow and Image Cytometry And Aptamersmentioning

confidence: 99%

Aptamer for imaging and therapeutic targeting of brain tumor glioblastoma

Delač

Motaln

Ulrich³

et al. 2015

Cytometry Pt A

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Aptamers are short single-stranded nucleic acids (RNA or ssDNA), identified by an in vitro selection process, denominated SELEX, from a partially random oligonucleotide library. They bind to a molecular target, a protein or other complex macromolecular structures of interest with high affinity and specificity, comparable to those of antibodies. Recently, aptamer selection protocols were developed for targeting living cells, including tumors. Chemical modifications of the aptamers and modalities of their detection and delivery systems are already available with high selectivity and targeting ability for the desired cancer cell type, making them promising for diagnosis and therapy. Glioblastoma multiformae represents the most malignant and fatal stage of glioma, and is also the most frequent brain tumor. Glioblastoma-specific aptamers were developed by either targeting the whole cell surface or known glioma biomarkers. These aptamers may gain importance for imaging, tumor cell isolation from biopsies and drug delivery. In biomedical imaging techniques, aptamers coupled with radionuclide or fluorescent labels, bioconjugates and nanoparticles offer an advanced, noninvasive manner for defining the glioblastoma tissue border. Though single modality aptamer imaging probes have some limitations, these are overcome by the use of multimodal probes. Due to selectivity and chemical characteristics, aptamers can be coupled to functionalized nanoparticles and loaded with a drug, appeared promising for in vivo targeting of glioblastoma. Finally, aptamers are effective mediators for gene silencing when coupled to small interfering RNA and a viral vector, thus providing a novel tool with enhanced targeting capability in drug delivery, designed for tailored treatment of glioblastoma patients. V C 2015 International Society for Advancement of Cytometry Key terms aptamer; SELEX; cancer biomarkers; cell and target protein; imaging; drug delivery; glioblastoma; nanoparticles APTAMERS AND THE SELEX TECHNOLOGY IN CANCER APTAMERS, first introduced by Tuerk and Gold (1) and Ellington and Szostak (2), are short chains of DNA or RNA oligonucleotides that bind to small molecules, peptides, and macromolecules, such as proteins of various sizes and conformations. These are identified by an in vitro selection method in a stepwise evolutionary process from a combinatorial library of partial randomized oligonucleotides, consisting of up to 10 15 different sequences and secondary and tertiary structures by a method called SELEX (Systematic Evolution of Ligands by EXponential enrichment). It begins with a pool of variable oligonucleotide sequences with multiple rounds of target exposure of the combinatorial RNA or DNA library, followed by elution of target binders and enrichment of those by RT-PCR or PCR procedures are performed. These results in exponential increase of oligonucleotides with improved ligand to target binding in the previously combinatorial library, which finally is narrowed down to a homogeneous population of high-affinity...

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“…13 Nanomaterials, such as quantum dots, which have quantum confined effects, are essential to develop highly bright and long-life fluorescence materials, which will be indispensable for molecular imaging 14,15 and in vivo imaging. [16][17][18] The research efforts in my laboratory have been focused on the development of novel nanobiodevices for biomedical applications. [1][2][3][4][5][6][7] In this review article, we mainly overview our research activities, including nanobiodevices for single-cell analysis, [20][21][22][23][24][25] single biomolecule analysis, 19,[26][27][28][29]41,46,50,[54][55][56][57][58] biomarker detections, 23,[30][31][32][33]39,[47][48][49] cancer theranostics, and iPS cell in vivo imaging.…”

Section: Introductionmentioning

confidence: 99%