<i>In Vitro</i> Seed Germination and Embryo Culture in <i>Nothapodytes foetida</i> (Wight) Sleumer

Abstract

ABSTRACT. In vitro seed germination and embryo culture have been achieved in Nothapodytes foetida, this plant is known for its rich source of anticancer drug i. e., Camptothecin. In present study both normal and decoated seeds were subjected to different treatments viz., H2O, GA 3 , H2O2, H2SO4, chlorine water and mechanical scarification, further these were germinated on water agar medium (WA), filter paper bridge (FB), half strength MS (HMS) and full strength MS (FMS) medium. The highest percentage (69%) of germination was achieved from decoated seeds treated with 10mg/L GA 3 and germinated on Filter Paper Bridge. And for embryo culture mature embryos were inoculated on MS medium containing various combination and concentrations of cytokinins (BAP, Kn and TDZ) and auxin (IAA and NAA) for rapid conversion into a plantlet. Among the different combinations of growth regulators; highest frequency (100%) of plantlet conversion was obtained on MS medium containing Kn (1.0mg/L) and NAA (0.2mg/L).

Standardization of zygotic embryo culture from Nerium oleander L. and comparative analysis of biosynthesized cardiac glycosides within in vitro and acclimatized plants

“…In the present study, the germination percentage of N. oleander zygotic embryos was found much higher than the wild mature seeds in the culture medium, which might be due to the presence of various inhibitors in the seed coat (Kaveri & Rao, 2015). However, some other direct (Karrunakaran & Shri, 2010;Hatzilazarou et al, 2019) and indirect in vitro micropropagation techniques are reported from this plant (DH Paper, 1989;Santos et al, 1994;Vila et al, 2010) yet no ZEC and CGs profiling of acclimatized and in vitro raised plants found to be reported in our knowledge.…”