&lt;I&gt;In Vitro&lt;/I&gt; Ruminal Fluid Fermentation as Influenced by Corn-Derived Dried Distillers' Grains with Solubles

Miśta, Dorota; Pecka, E.; Zachwieja, Andrzej; Zawadzki, Wojciech; Bodarski, R.; Paczyńska, Katarzyna; Tumanowicz, Joanna; Kupczyński, Robert; Adamski, Maciej

doi:10.3409/fb62_4.345

Cited by 11 publications

(18 citation statements)

References 19 publications

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“…In our study, a slight decrease in methanogenesis was found in group D3 in the 24-hour fermentation period, where the lowest pH was observed. The effect of the fermentation time on pH value in the analysed samples was noticed, and it corresponds to earlier research (Miśta et al 2014). …”

Section: Discussionmentioning

confidence: 68%

“…However, the effect of DDGS on the increase in total gas production was observed in this study. Conversely, earlier in vitro research in cows showed a decrease in both methanogenesis and total gas production (Miśta et al 2014). The greatest drop in gas production was observed in the samples containing 100% DDGS in In vitro study of the effect of corn dried distillers grains...…”

Section: Discussionmentioning

confidence: 99%

“…The previous study in cows showed a decrease in total SCFA concentration in ruminal fluid under the influence of DDGS content in the fermentation substrate (Miśta et al 2014). In lambs, the kcal energy available from the SCFAs produced per milligram of digested DM decreased as corn was replaced with DDGS (Behlke et al 2008).…”

Section: Discussionmentioning

confidence: 99%

“…Growing DDGS content caused a linear decrease in rumen methanogenesis in both in vivo (Benchaar et al 2013) and in vitro studies (Miśta et al 2014). The research conducted by Behlke (2007) showed that corn DDGS included in diet fed to heifers as a replacement for the forage also lowered the methane emission.…”

Section: Discussionmentioning

confidence: 99%

“…One of these feed additives is dried distillers grains with solubles (DDGS) considered as the modulator of rumen fermentation profile and inhibitor of methane production. However, most of the research concerning the effect of DDGS on rumen fermentation was performed on cattle (Kleinschmit et al 2006, Behlke et al 2008, McGinn et al 2009, Zhang et al 2010, Morvay et al 2011, Morrow 2012, Hünerberg et al 2013, Segers et al 2013, Miśta et al 2014. Research concerning sheep rumen fermentation influenced by DDGS addition is limited , Radev 2012).…”

Section: Introductionmentioning

confidence: 99%

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In vitro study of the effect of corn dried distillers grains with solubles on rumen fermentation in sheep

Pecka-Kiełb¹,

Zawadzki²,

Zachwieja³

et al. 2015

Polish Journal of Veterinary Sciences

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The aim of the in vitro study was to determine the effect of corn dried distillers grains with solubles (corn DDGS), used as a replacement for the concentrate ingredients of sheep diet, on rumen fermentation. The material for the study was the ruminal fluid of Polish Merino sheep which was incubated during 4-, 8-or 24-hour periods. Five groups of samples were prepared for in vitro fermentation: C -control, incubated with the substrate consisting of the concentrate ingredients; D1, D2 and D3, where DDGS was used as a substrate added in proportions of 10, 20 and 30% of dry matter of the concentrate; and D4, where 100% DDGS was used as a substrate. After fermentation, the gas and short chain fatty acids (SCFAs) analyses were performed using gas chromatography. The ammonia concentration and pH were also determined, and the SCFA utilization index (NGR), the fermentation efficiency (FE) and the index of cell yield of ruminal microorganisms (CY) were calculated. This research showed no effect of DDGS on the methane emission. The positive correlations between the amount of methane and ammonia concentrations in the 8-and 24-hour fermentation periods were found. DDGS addition increased propionate proportion, but decreased production of acetate (p<0.01). Additionally, D1, D2, D3 and D4 substrates lowered isobutyrate (p<0.05) and isovalerate (p<0.01) production. Based on the results obtained, it can be stated that partial substitution of the concentrate ingredients with DDGS did not have deleterious effect on sheep rumen fermentation processes.

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Section: Discussionmentioning

confidence: 68%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

In vitro study of the effect of corn dried distillers grains with solubles on rumen fermentation in sheep

Pecka-Kiełb¹,

Zawadzki²,

Zachwieja³

et al. 2015

Polish Journal of Veterinary Sciences

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Associative effects of wet distiller’s grains plus solubles and tannin-rich peanut skin supplementation on in vitro rumen fermentation, greenhouse gas emissions, and microbial changes1

Min

Castleberry

Allen

et al. 2019

Journal of Animal Science

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Two sets of in vitro rumen fermentation experiments were conducted to determine effects of diets that included wet distiller’s grains plus solubles (WDGS) and tannin-rich peanut skin (PS) on the in vitro digestibility, greenhouse gas (GHG) and other gas emissions, fermentation rate, and microbial changes. The objectives were to assess associative effects of various levels of PS or WDGS on the in vitro digestibility, GHG and other gas emissions, fermentation rate, and microbial changes in the rumen. All gases were collected using an ANKOM Gas Production system for methane (CH4), carbon dioxide (CO2), nitrous oxide (N2O), and hydrogen sulfide (H2S) analyses. Cumulative ruminal gas production was determined using 250 mL ANKOM sampling bottles containing 50 mL of ruminal fluid (pH 5.8), 40 mL of artificial saliva (pH 6.8), and 6 g of mixed diets after a maximum of 24 h of incubation. Fermenters were flushed with CO2 gas and held at 39 °C in a shaking incubator for 24 h. Triplicate quantitative real-time polymerase chain reaction (qPCR) analyses were conducted to determine microbial diversity. When WDGS was supplied in the diet, in the absence of PS, cumulative CH4 production increased (P < 0.05) with 40% WDGS. In the presence of PS, production of CH4 was reduced but the reduction was less at 40% WDGS. In the presence of PS, ruminal lactate, succinate, and acetate/propionate (A/P) ratio tended to be less with a WDGS interaction (P < 0.01). In the presence of PS and with 40% WDGS, average populations of Bacteroidetes, total methanogens, Methanobrevibacter sp. AbM4, and total protozoa were less. The population of total methanogens (R2 = 0.57; P < 0.01), Firmicutes (R2 = 0.46: P < 0.05), and Firmicutes/Bacteroidetes (F/B) ratio (R2 = 0.46; P < 0.03) were strongly correlated with ruminal CH4 production. Therefore, there was an associative effect of tannin-rich PS and WDGS, which suppressed methanogenesis both directly and indirectly by modifying populations of ruminal methanogens.

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In vitro ruminal fermentation and in situ ruminal degradation of tamarind kernel powder extract residue in wethers

Wang

Oishi

Sato

et al. 2016

Animal Science Journal

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We examined the in vitro fermentation characteristics and in situ dry matter (DM) and crude protein (CP) degradability of tamarind kernel powder extract residue (TKPER), a by-product of polysaccharides thickener processing. Two types of TKPER (I and II), of which the CP and neutral detergent fiber organic matter basis contents (%) were 41.4 and 42.0 and 1.4 and 0.5, respectively, were compared with dry heat soybean (SB), soybean meal (SBM) and dry soybean curd residue (SBCR). The TKPERs had significantly lower in vitro gas production compared to the other products at each observation time (P < 0.05). The in vitro DM and CP digestibility (%) of TKPER I and II were 67.7 and 64.9, and 64.5 and 58.0, respectively, significantly lower than those of SB and SBM (P < 0.05). We used four wethers (55.6 ± 4.6 kg) with ruminal cannulas to investigate the in situ degradability of TKPER I, SB, SBM and SBCR. At the ruminal outflow rate of 0.05/h, the effective degradability (%) values of the DM and CP of TKPER I were 64.1 and 49.7, respectively, which were similar to those of SBM. In conclusion, TKPER had high CP and exhibited in situ degradability similar to that of SBM, suggesting that TKPER could be used as a protein source feed.

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<I>In Vitro</I> Ruminal Fluid Fermentation as Influenced by Corn-Derived Dried Distillers' Grains with Solubles

Cited by 11 publications

References 19 publications

In vitro study of the effect of corn dried distillers grains with solubles on rumen fermentation in sheep

In vitro study of the effect of corn dried distillers grains with solubles on rumen fermentation in sheep

Associative effects of wet distiller’s grains plus solubles and tannin-rich peanut skin supplementation on in vitro rumen fermentation, greenhouse gas emissions, and microbial changes1

In vitro ruminal fermentation and in situ ruminal degradation of tamarind kernel powder extract residue in wethers

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